Restriction map for pF1KE0145
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AluI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstF5I      BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
EaeI        EagI        EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HaeII       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspA1I      
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tru9I       Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         133  CGCG                 CG^CG
AcyI           52  GGCGTC               G[GA]^CG[CT]C
AflIII        266  ACGTGT               A^C[GA][CT]GT
AhdI          172  GACAAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI           72  GATCC                GGATC(4/5)
AspEI         172  GACAAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI           49  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII         101  GGGCTC               G[GA]GC[CT]^C
BbrPI         265  CACGTG               CAC^GTG
BbsI           55  GTCTTC               GAAGAC(2/6)
BcgI          119  GCAAGGAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI           98  CCGGG                CC^[GC]GG
BmyI          101  GGGCTC               G[AGT]GC[ACT]^C
BpuAI          55  GTCTTC               GAAGAC(2/6)
BsaAI         265  CACGTG               [CT]AC^GT[GA]
BsaBI          72  GATCCGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          52  GGCGTC               G[GA]^CG[CT]C
Bsp1286I      101  GGGCTC               G[AGT]GC[ACT]^C
BsrBI          93  CCGCTC               CCGCTC(-3/-3)
BstEII        351  GGTCACC              G^GT[ACGT]ACC
BstNI         356  CCTGG                CC^[AT]GG
BstPI         351  GGTCACC              G^GT[ACGT]ACC
BstUI         133  CGCG                 CG^CG
DrdI          343  GACTACGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          281  CCATGG               C^C[GA][CT]GG
Eam1105I      172  GACAAAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       351  GGTCACC              G^GT[ACGT]ACC
EcoRII        356  CCTGG                ^CC[AT]GG
EcoT14I       281  CCATGG               C^C[AT][AT]GG
HaeIII        285  GGCC                 GG^CC
HgaI           53  GCGTC                GACGC(5/10)
Hin1I          52  GGCGTC               G[GA]^CG[CT]C
HphI          353  TCACC                GGTGA(8/7)
MaeII         266  ACGT                 A^CGT
MaeIII        352  GTCAC                ^GT[ACGT]AC
MamI           72  GATCCGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI          356  CCTGG                CC^[AT]GG
MvnI          133  CGCG                 CG^CG
NciI           98  CCGGG                CC^[GC]GG
NcoI          281  CCATGG               C^CATGG
NspI          188  ACATGC               [GA]CATG^[CT]
PflFI          49  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         265  CACGTG               CAC^GTG
PmlI          265  CACGTG               CAC^GTG
PstI          110  CTGCAG               CTGCA^G
SfcI          110  CTGCAG               C^T[GA][CT]AG
StyI          281  CCATGG               C^C[AT][AT]GG
Tsp45I        352  GTCAC                ^GT[GC]AC
Tth111I        49  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AvaII          12  GGACC                G^G[AT]CC
              292  GGTCC               
BbvI          109  GCTGC                GCAGC(8/12)
              203  GCTGC               
BssKI          98  CCGGG                ^CC[ACGT]GG
              356  CCTGG               
Cac8I         227  GCGGGC               GC[ACGT]^[ACGT]GC
              303  GCACGC              
CfoI          132  GCGC                 GCG^C
              231  GCGC                
Cfr13I         12  GGACC                G^G[ACGT]CC
              292  GGTCC               
DdeI          149  CTGAG                C^T[ACGT]AG
              317  CTGAG               
DpnII          72  GATC                 ^GATC
              126  GATC                
FauI           92  CCCGC                CCCGC(4/6)
              227  GCGGG               
HapII          98  CCGG                 C^CGG
              106  CCGG                
HhaI          132  GCGC                 GCG^C
              231  GCGC                
HinP1I        132  GCGC                 G^CGC
              231  GCGC                
HpaII          98  CCGG                 C^CGG
              106  CCGG                
MboI           72  GATC                 ^GATC
              126  GATC                
MboII         258  GAAGA                GAAGA(8/7)
               56  TCTTC               
MspI           98  CCGG                 C^CGG
              106  CCGG                
NdeII          72  GATC                 ^GATC
              126  GATC                
NlaIV          87  GGCTCC               GG[ACGT]^[ACGT]CC
              102  GGCTCC              
PleI           43  GACTC                GAGTC(4/5)
              246  GAGTC               
Sau3AI         72  GATC                 ^GATC
              126  GATC                
Sau96I         12  GGACC                G^G[ACGT]CC
              292  GGTCC               
ScrFI          98  CCGGG                CC^[ACGT]GG
              356  CCTGG               
TfiI           77  GAATC                G^A[AT]TC
              261  GATTC               
TseI          109  GCTGC                G^C[AT]GC
              203  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AciI        AfaI        BsaJI       BsiYI       BslI        
Fnu4HI      HinfI       ItaI        MnlI        MwoI        
NlaIII      RsaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com