(Circular) (Six-base) MAPSORT of: pF1KE0145.seq  Check: 3556  from: 1  to: 3488



pF1KE0145  3488 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:    526     526 
   Size:       3488
 
Acc65I G'GTAC_C

Cuts at:    504     504 
   Size:       3488
 
AclI AA'CG_TT

Cuts at:   3328    3328 
   Size:       3488
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1313    1745    2492    2760    1313 
   Size:        432     747     268    2041

  Fragments arranged by size: 

                2041     747     432     268
 
AflIII A'CryG_T

Cuts at:    374     840    1944    2921     374 
   Size:        466    1104     977     941

  Fragments arranged by size: 

                1104     977     941     466
 
AgeI A'CCGG_T

Cuts at:   1876    1876 
   Size:       3488
 
AhdI GACnn_n'nnGTC

Cuts at:    285     285 
   Size:       3488
 
AlwNI CAG_nnn'CTG

Cuts at:    793    2360     793 
   Size:       1567    1921
 
ApaLI G'TGCA_C

Cuts at:   2258    2258 
   Size:       3488
 
ApoI r'AATT_y

Cuts at:    492    2841    3097     492 
   Size:       2349     256     883

  Fragments arranged by size: 

                2349     883     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3488
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3488
 
AvaI C'yCGr_G

Cuts at:    508     508 
   Size:       3488
 
BamHI G'GATC_C

Cuts at:    513     513 
   Size:       3488
 
BanI G'GyrC_C

Cuts at:    504     918    1166    1201     504 
   Size:        414     248      35    2791

  Fragments arranged by size: 

                2791     414     248      35
 
BanII G_rGCy'C

Cuts at:    213     502    1532     213 
   Size:        289    1030    2169

  Fragments arranged by size: 

                2169    1030     289
 
BbeI G_GCGC'C

Cuts at:    922    1170     922 
   Size:        248    3240
 
BbsI GAAGACnn'nnnn_

Cuts at:    156     156 
   Size:       3488
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    216     250     216 
   Size:         34    3454
 
BciVI GTATCCnnnnn_n'

Cuts at:   1386    2147    3488    1386 
   Size:        761    1341    1386

  Fragments arranged by size: 

                1386    1341     761
 
BclI T'GATC_A

Cuts at:   1008    1008 
   Size:       3488
 
BglII A'GATC_T

Cuts at:   1003    3040    1003 
   Size:       2037    1451
 
BlpI GC'TnA_GC

Cuts at:    600     600 
   Size:       3488
 
Bme1580I G_kGCm'C

Cuts at:   1113    1206    2262    1113 
   Size:         93    1056    2339

  Fragments arranged by size: 

                2339    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    700     781    1116    3260     700 
   Size:         81     335    2144     928

  Fragments arranged by size: 

                2144     928     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1855    1855 
   Size:       3488
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    664    1543    2035    2333     664 
   Size:        879     492     298    1819

  Fragments arranged by size: 

                1819     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3192    3192 
   Size:       3488
 
BsaAI yAC'GTr

Cuts at:    375     841     375 
   Size:        466    3022
 
BsaBI GATnn'nnATC

Cuts at:      6     184    1002    3039       6 
   Size:        178     818    2037     455

  Fragments arranged by size: 

                2037     818     455     178
 
BsaHI Gr'CG_yC

Cuts at:    161     919    1167    1869    3365     161 
   Size:        758     248     702    1496     284

  Fragments arranged by size: 

                1496     758     702     284     248
 
BsaWI w'CCGG_w

Cuts at:    677    1198    1876    2150    2297    2708     677 
   Size:        521     678     274     147     411    1457

  Fragments arranged by size: 

                1457     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    136     166     136 
   Size:         30    3458
 
BseYI C'CCAG_C

Cuts at:    407     913    1671    2248    2744     407 
   Size:        506     758     577     496    1151

  Fragments arranged by size: 

                1151     758     577     506     496
 
BsiEI CG_ry'CG

Cuts at:    104    1030    1076    2284     104 
   Size:        926      46    1208    1308

  Fragments arranged by size: 

                1308    1208     926      46
 
BsiHKAI G_wGCw'C

Cuts at:    502    1280    1470    2262     502 
   Size:        778     190     792    1728

  Fragments arranged by size: 

                1728     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    213     502    1113    1206    1280    1470    1532    2262     213 
   Size:        289     611      93      74     190      62     730    1439

  Fragments arranged by size: 

                1439     730     611     289     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2708    2708 
   Size:       3488
 
BspHI T'CATG_A

Cuts at:   2664    2664 
   Size:       3488
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    538     752    1054    1435     538 
   Size:        214     302     381    2591

  Fragments arranged by size: 

                2591     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     203    1780    1834    3318    3483      50 
   Size:        153    1577      54    1484     165      55

  Fragments arranged by size: 

                1577    1484     165     153      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1400    1400 
   Size:       3488
 
BsrFI r'CCGG_y

Cuts at:   1486    1667    1876    1486 
   Size:        181     209    3098

  Fragments arranged by size: 

                3098     209     181
 
BssSI C'ACGA_G

Cuts at:   1759    2117    1759 
   Size:        358    3130
 
BstAPI GCAn_nnn'nTGC

Cuts at:    865     865 
   Size:       3488
 
BstBI TT'CG_AA

Cuts at:   1849    1849 
   Size:       3488
 
BstEII G'GTnAC_C

Cuts at:    459     459 
   Size:       3488
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     114 
   Size:       3488
 
BstYI r'GATC_y

Cuts at:      1     513     980    1003    1338    1584    2596    2682    2694 
   Size:        512     467      23     335     246    1012      86      12

Cuts at:   2694    3040       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     512     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     389     107 
   Size:        282    3206
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1438    1619    1782    1438 
   Size:        181     163    3144

  Fragments arranged by size: 

                3144     181     163
 
BtsI GCAGTG_nn'

Cuts at:    731     731 
   Size:       3488
 
DraI TTT'AAA

Cuts at:    487     487 
   Size:       3488
 
DrdI GACnn_nn'nnGTC

Cuts at:    457    1194    2052     457 
   Size:        737     858    1893

  Fragments arranged by size: 

                1893     858     737
 
EaeI y'GGCC_r

Cuts at:   1073    1247    1638    1665    3010    1073 
   Size:        174     391      27    1345    1551

  Fragments arranged by size: 

                1551    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1073    1073 
   Size:       3488
 
EarI CTCTTCn'nnn_

Cuts at:   1511    1721    1511 
   Size:        210    3278
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    133    1696    2006    2152    3298     133 
   Size:       1563     310     146    1146     323

  Fragments arranged by size: 

                1563    1146     323     310     146
 
EcoICRI GAG'CTC

Cuts at:    500     500 
   Size:       3488
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1313    1745    2492    2760    1313 
   Size:        432     747     268    2041

  Fragments arranged by size: 

                2041     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    627     627 
   Size:       3488
 
EcoRI G'AATT_C

Cuts at:    492     492 
   Size:       3488
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1507    1507 
   Size:       3488
 
FspI TGC'GCA

Cuts at:   1269    1269 
   Size:       3488
 
HaeII r_GCGC'y

Cuts at:    922    1170    2192    3171     922 
   Size:        248    1022     979    1239

  Fragments arranged by size: 

                1239    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    136     168     271     303    1330    1360    1362    1392     136 
   Size:         32     103      32    1027      30       2      30    2232

  Fragments arranged by size: 

                2232    1027     103      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    527     527 
   Size:       3488
 
KasI G'GCGC_C

Cuts at:    918    1166     918 
   Size:        248    3240
 
KpnI G_GTAC'C

Cuts at:    508     508 
   Size:       3488
 
MluI A'CGCG_T

Cuts at:   2921    2921 
   Size:       3488
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    179    2159    2343     179 
   Size:       1980     184    1324

  Fragments arranged by size: 

                1980    1324     184
 
MscI TGG'CCA

Cuts at:   1249    1249 
   Size:       3488
 
MslI CAynn'nnrTG

Cuts at:    112    1477    1604     112 
   Size:       1365     127    1996

  Fragments arranged by size: 

                1996    1365     127
 
MspA1I CmG'CkG

Cuts at:    599     913    1273    2286    2531     599 
   Size:        314     360    1013     245    1556

  Fragments arranged by size: 

                1556    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1669    1669 
   Size:       3488
 
NarI GG'CG_CC

Cuts at:    919    1167     919 
   Size:        248    3240
 
NcoI C'CATG_G

Cuts at:    107     389     107 
   Size:        282    3206
 
NgoMIV G'CCGG_C

Cuts at:   1667    1667 
   Size:       3488
 
NspI r_CATG'y

Cuts at:    300     541    1041    1948     300 
   Size:        241     500     907    1840

  Fragments arranged by size: 

                1840     907     500     241
 
PciI A'CATG_T

Cuts at:   1944    1944 
   Size:       3488
 
PmeI GTTT'AAAC

Cuts at:    487     487 
   Size:       3488
 
PmlI CAC'GTG

Cuts at:    375     375 
   Size:       3488
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2627    2659    2627 
   Size:         32    3456
 
PstI C_TGCA'G

Cuts at:    222     535    1220     222 
   Size:        313     685    2490

  Fragments arranged by size: 

                2490     685     313
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3488
 
PvuII CAG'CTG

Cuts at:    913    1273     913 
   Size:        360    3128
 
RsrII CG'GwC_CG

Cuts at:   1683    1683 
   Size:       3488
 
SacI G_AGCT'C

Cuts at:    502     502 
   Size:       3488
 
SalI G'TCGA_C

Cuts at:    525     525 
   Size:       3488
 
SapI GCTCTTCn'nnn_

Cuts at:   1511    1721    1511 
   Size:        210    3278
 
SbfI CC_TGCA'GG

Cuts at:    535     535 
   Size:       3488
 
ScaI AGT'ACT

Cuts at:   3005    3005 
   Size:       3488
 
SfcI C'TryA_G

Cuts at:     33     218     531    1216    2209    2400      33 
   Size:        185     313     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     313     191     185
 
SfoI GGC'GCC

Cuts at:    920    1168     920 
   Size:        248    3240
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3488
 
SmaI CCC'GGG

Cuts at:    510     510 
   Size:       3488
 
SmlI C'TyrA_G

Cuts at:    643    1558    2050    2312     643 
   Size:        915     492     262    1819

  Fragments arranged by size: 

                1819     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3488
 
SphI G_CATG'C

Cuts at:    541    1041     541 
   Size:        500    2988
 
StyI C'CwwG_G

Cuts at:    107     389     622     976     107 
   Size:        282     233     354    2619

  Fragments arranged by size: 

                2619     354     282     233
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1071    1204    1663    1872    1071 
   Size:        133     459     209    2687

  Fragments arranged by size: 

                2687     459     209     133
 
TatI w'GTAC_w

Cuts at:   3003    3003 
   Size:       3488
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3005    3037    3005 
   Size:         32    3456
 
Tth111I GACn'n_nGTC

Cuts at:    160    1285     160 
   Size:       1125    2363
 
XbaI T'CTAG_A

Cuts at:    519     519 
   Size:       3488
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    983     983 
   Size:       3488
 
XmaI C'CCGG_G

Cuts at:    508     508 
   Size:       3488
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PmlI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI
     BpmI   Bpu10I    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI