Restriction map for pF1KB7084
 (Linear) (Six-base) MAPSORT of: KIBB7084.seq  Check: 2472  from: 1  to: 381



KIBB7084.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AfeI AGC'GCT

Cuts at:      0      88     381 
   Size:         88     293
 
AleI CACnn'nnGTG

Cuts at:      0     223     381 
   Size:        223     158
 
ApaI G_GGCC'C

Cuts at:      0     348     381 
   Size:        348      33
 
AvaI C'yCGr_G

Cuts at:      0     358     381 
   Size:        358      23
 
AvrII C'CTAG_G

Cuts at:      0     297     381 
   Size:        297      84
 
BanI G'GyrC_C

Cuts at:      0     203     381 
   Size:        203     178
 
BanII G_rGCy'C

Cuts at:      0     158     348     381 
   Size:        158     190      33

  Fragments arranged by size: 

                 190     158      33
 
BbeI G_GCGC'C

Cuts at:      0     207     381 
   Size:        207     174
 
BlpI GC'TnA_GC

Cuts at:      0     157     381 
   Size:        157     224
 
Bme1580I G_kGCm'C

Cuts at:      0     136     348     381 
   Size:        136     212      33

  Fragments arranged by size: 

                 212     136      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     381 
   Size:         64     317
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      13     381 
   Size:         13     368
 
BsaHI Gr'CG_yC

Cuts at:      0     204     381 
   Size:        204     177
 
BsiEI CG_ry'CG

Cuts at:      0     144     381 
   Size:        144     237
 
BsiHKAI G_wGCw'C

Cuts at:      0      29     158     199     381 
   Size:         29     129      41     182

  Fragments arranged by size: 

                 182     129      41      29
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     136     158     199     348     381 
   Size:         29     107      22      41     149      33

  Fragments arranged by size: 

                 149     107      41      33      29      22
 
BsrFI r'CCGG_y

Cuts at:      0     143     381 
   Size:        143     238
 
BssSI C'ACGA_G

Cuts at:      0     373     381 
   Size:        373       8
 
BstEII G'GTnAC_C

Cuts at:      0     225     381 
   Size:        225     156
 
BstYI r'GATC_y

Cuts at:      0     270     381 
   Size:        270     111
 
BtgI C'CryG_G

Cuts at:      0     179     381 
   Size:        179     202
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     375     381 
   Size:        375       6
 
EaeI y'GGCC_r

Cuts at:      0     141     381 
   Size:        141     240
 
EagI C'GGCC_G

Cuts at:      0     141     381 
   Size:        141     240
 
EarI CTCTTCn'nnn_

Cuts at:      0     124     381 
   Size:        124     257
 
EcoICRI GAG'CTC

Cuts at:      0     156     381 
   Size:        156     225
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     381 
   Size:         64     317
 
EcoO109I rG'GnC_Cy

Cuts at:      0     368     381 
   Size:        368      13
 
FseI GG_CCGG'CC

Cuts at:      0     147     381 
   Size:        147     234
 
HaeII r_GCGC'y

Cuts at:      0      90     207     381 
   Size:         90     117     174

  Fragments arranged by size: 

                 174     117      90
 
KasI G'GCGC_C

Cuts at:      0     203     381 
   Size:        203     178
 
MslI CAynn'nnrTG

Cuts at:      0     223     381 
   Size:        223     158
 
MspA1I CmG'CkG

Cuts at:      0      56      72     257     381 
   Size:         56      16     185     124

  Fragments arranged by size: 

                 185     124      56      16
 
NaeI GCC'GGC

Cuts at:      0     145     381 
   Size:        145     236
 
NarI GG'CG_CC

Cuts at:      0     204     381 
   Size:        204     177
 
NgoMIV G'CCGG_C

Cuts at:      0     143     381 
   Size:        143     238
 
PpuMI rG'GwC_Cy

Cuts at:      0     368     381 
   Size:        368      13
 
PspOMI G'GGCC_C

Cuts at:      0     344     381 
   Size:        344      37
 
PstI C_TGCA'G

Cuts at:      0      71     116     381 
   Size:         71      45     265

  Fragments arranged by size: 

                 265      71      45
 
PvuII CAG'CTG

Cuts at:      0      72     381 
   Size:         72     309
 
SacI G_AGCT'C

Cuts at:      0     158     381 
   Size:        158     223
 
SanDI GG'GwC_CC

Cuts at:      0     368     381 
   Size:        368      13
 
SapI GCTCTTCn'nnn_

Cuts at:      0     124     381 
   Size:        124     257
 
SfcI C'TryA_G

Cuts at:      0      67     112     381 
   Size:         67      45     269

  Fragments arranged by size: 

                 269      67      45
 
SfoI GGC'GCC

Cuts at:      0     205     381 
   Size:        205     176
 
SmlI C'TyrA_G

Cuts at:      0      28     381 
   Size:         28     353
 
StuI AGG'CCT

Cuts at:      0     302     381 
   Size:        302      79
 
StyI C'CwwG_G

Cuts at:      0     128     149     297     381 
   Size:        128      21     148      84

  Fragments arranged by size: 

                 148     128      84      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     244     381 
   Size:        244     137
 

 Enzymes that do cut:

     AfeI     AleI     ApaI     AvaI    AvrII     BanI    BanII     BbeI
     BlpI Bme1580I     BpmI    BpuEI    BsaHI    BsiEI  BsiHKAI Bsp1286I
    BsrFI    BssSI   BstEII    BstYI     BtgI     DrdI     EaeI     EagI
     EarI  EcoICRI  Eco57MI EcoO109I     FseI    HaeII     KasI     MslI
   MspA1I     NaeI     NarI   NgoMIV    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SapI     SfcI     SfoI     SmlI     StuI     StyI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EciI    EcoNI
    EcoRI    EcoRV     FalI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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