Restriction map for pF1KB6309
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BcgI        BciVI       BfrI        BglII       
BlnI        BpuAI       BsaBI       BsaHI       BsaI        
BseAI       BsgI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DrdI        DsaI        Eam1105I    Eco47III    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MunI        NarI        NcoI        
NdeI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tsp509I     Tth111I     XbaI        XcmI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AfaI          273  GTAC                 GT^AC
AlwNI          46  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         267  GAGCAC               G[AT]GC[AT]^C
BbvCI         367  GCTGAGG              CCTCAGC(-5/-2)
BclI          329  TGATCA               T^GATCA
BlpI          206  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      206  GCTTAGC              GC^T[ACGT]AGC
BsaAI         270  CACGTA               [CT]AC^GT[GA]
BsaWI         284  ACCGGA               [AT]^CCGG[AT]
BseRI         358  GAGGAG               GAGGAG(10/8)
BsiEI         728  CGGCCG               CG[GA][CT]^CG
BsiHKAI       267  GAGCAC               G[AT]GC[AT]^C
BsiWI         272  CGTACG               C^GTACG
BsmBI         150  CGTCTC               CGTCTC(1/5)
BsmFI         407  GGGAC                GGGAC(10/14)
BsrBI         499  GAGCGG               CCGCTC(-3/-3)
BsrFI           9  GCCGGC               [GA]^CCGG[CT]
BsrI          610  ACTGG                ACTGG(1/-1)
BssSI         244  CTCGTG               CACGAG(-5/-1)
BstF5I        414  CATCC                GGATG(2/0)
CelII         206  GCTTAGC              GC^T[ACGT]AGC
Cfr10I          9  GCCGGC               [GA]^CCGG[CT]
DraIII        548  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          728  CGGCCG               C^GGCCG
EarI          472  CTCTTC               CTCTTC(1/4)
EclXI         728  CGGCCG               C^GGCCG
Eco52I        728  CGGCCG               C^GGCCG
EcoNI         489  CCTCCTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       402  CCAAGG               C^C[AT][AT]GG
FbaI          329  TGATCA               T^GATCA
FokI          414  CATCC                GGATG(9/13)
Ksp632I       472  CTCTTC               CTCTTC(1/4)
MaeII         271  ACGT                 A^CGT
MseI          338  TTAA                 T^TAA
NaeI            9  GCCGGC               GCC^GGC
NgoMIV          9  GCCGGC               G^CCGGC
NlaIII         92  CATG                 CATG^
NotI          727  GCGGCCGC             GC^GGCCGC
NspI           91  GCATGC               [GA]CATG^[CT]
PaeR7I         36  CTCGAG               C^TCGAG
PflMI          45  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI          273  GTAC                 GT^AC
SfcI          493  CTACAG               C^T[GA][CT]AG
SfiI            3  GGCCGCGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI           15  CCCGGG               CCC^GGG
SmlI           36  CTCGAG               C^T[CT][GA]AG
SphI           91  GCATGC               GCATG^C
StyI          402  CCAAGG               C^C[AT][AT]GG
Tru9I         338  TTAA                 T^TAA
TspRI         525  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I         45  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI           36  CTCGAG               C^TCGAG
XmaCI          15  CCCGGG               C^CCGGG
XmaI           15  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          312  GATCC                GGATC(4/5)
              435  GATCC               
ApaI          107  GGGCCC               GGGCC^C
              447  GGGCCC              
AvaII         651  GGACC                G^G[AT]CC
              684  GGTCC               
BanII         107  GGGCCC               G[GA]GC[CT]^C
              447  GGGCCC              
BglI            4  GCCGCGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              131  GCCTGTCGGGC         
BpmI          300  CTCCAG               CTGGAG(16/14)
              611  CTGGAG              
BsmAI         390  GAGAC                GTCTC(1/5)
              151  GTCTC               
BspMI         428  ACCTGC               ACCTGC(4/8)
              463  ACCTGC              
BssHII         84  GCGCGC               G^CGCGC
              211  GCGCGC              
DraII         106  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              447  GGGCCCC             
EaeI            2  TGGCCG               [CT]^GGCC[GA]
              728  CGGCCG              
EcoO109I      106  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              447  GGGCCCC             
HaeII          60  GGCGCT               [GA]GCGC^[CT]
              239  AGCGCC              
HgaI          219  GACGC                GACGC(5/10)
              288  GACGC               
HinfI         409  GACTC                G^A[ACGT]TC
              529  GACTC               
HphI          327  GGTGA                GGTGA(8/7)
              332  TCACC               
MslI           24  CATCTCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              597  CATCTTTGTG          
PleI          409  GACTC                GAGTC(4/5)
              529  GACTC               
PvuII          70  CAGCTG               CAG^CTG
              626  CAGCTG              
SfaNI         156  GATGC                GCATC(5/9)
              291  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        BbvI        
BcnI        BfaI        BmyI        BsaJI       BsiYI       
BslI        BsmI        BsoBI       Bsp1286I    BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NheI        NlaIV       
Sau3AI      Sau96I      ScrFI       TaqI        TseI        
Tsp45I      TthHB8I     
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