Restriction map for pF1KB6309
 (Linear) (Six-base) MAPSORT of: KIBB6309.seq  Check: 8977  from: 1  to: 774



KIBB6309.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     472     774 
   Size:        472     302
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655     774 
   Size:        655     119
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     111     774 
   Size:        111     663
 
AlwNI CAG_nnn'CTG

Cuts at:      0      51     774 
   Size:         51     723
 
ApaI G_GGCC'C

Cuts at:      0     111     451     774 
   Size:        111     340     323

  Fragments arranged by size: 

                 340     323     111
 
AvaI C'yCGr_G

Cuts at:      0      15      36     343     774 
   Size:         15      21     307     431

  Fragments arranged by size: 

                 431     307      21      15
 
BanII G_rGCy'C

Cuts at:      0     111     451     774 
   Size:        111     340     323

  Fragments arranged by size: 

                 340     323     111
 
BbvCI CC'TCA_GC

Cuts at:      0     368     774 
   Size:        368     406
 
BclI T'GATC_A

Cuts at:      0     329     774 
   Size:        329     445
 
BglI GCCn_nnn'nGGC

Cuts at:      0      10     137     774 
   Size:         10     127     637

  Fragments arranged by size: 

                 637     127      10
 
BlpI GC'TnA_GC

Cuts at:      0     207     774 
   Size:        207     567
 
Bme1580I G_kGCm'C

Cuts at:      0     111     251     451     752     774 
   Size:        111     140     200     301      22

  Fragments arranged by size: 

                 301     200     140     111      22
 
BmtI G_CTAG'C

Cuts at:      0      67     163     206     774 
   Size:         67      96      43     568

  Fragments arranged by size: 

                 568      96      67      43
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285     632     774 
   Size:        285     347     142

  Fragments arranged by size: 

                 347     285     142
 
Bpu10I CC'TnA_GC

Cuts at:      0     368     774 
   Size:        368     406
 
BsaAI yAC'GTr

Cuts at:      0     272     774 
   Size:        272     502
 
BsaWI w'CCGG_w

Cuts at:      0     284     774 
   Size:        284     490
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     373     774 
   Size:        373     401
 
BseYI C'CCAG_C

Cuts at:      0      51      80     222     774 
   Size:         51      29     142     552

  Fragments arranged by size: 

                 552     142      51      29
 
BsiEI CG_ry'CG

Cuts at:      0     731     774 
   Size:        731      43
 
BsiHKAI G_wGCw'C

Cuts at:      0     271     774 
   Size:        271     503
 
BsiWI C'GTAC_G

Cuts at:      0     272     774 
   Size:        272     502
 
BsmI GAATG_Cn'

Cuts at:      0     397     585     762     774 
   Size:        397     188     177      12

  Fragments arranged by size: 

                 397     188     177      12
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     156     774 
   Size:        156     618
 
Bsp1286I G_dGCh'C

Cuts at:      0     111     251     271     451     752     774 
   Size:        111     140      20     180     301      22

  Fragments arranged by size: 

                 301     180     140     111      22      20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     437     472     774 
   Size:        437      35     302

  Fragments arranged by size: 

                 437     302      35
 
BsrBI CCG'CTC

Cuts at:      0     501     774 
   Size:        501     273
 
BsrFI r'CCGG_y

Cuts at:      0       9     774 
   Size:          9     765
 
BssHII G'CGCG_C

Cuts at:      0      84     211     774 
   Size:         84     127     563

  Fragments arranged by size: 

                 563     127      84
 
BssSI C'ACGA_G

Cuts at:      0     244     774 
   Size:        244     530
 
DraIII CAC_nnn'GTG

Cuts at:      0     553     774 
   Size:        553     221
 
EaeI y'GGCC_r

Cuts at:      0       2     728     774 
   Size:          2     726      46

  Fragments arranged by size: 

                 726      46       2
 
EagI C'GGCC_G

Cuts at:      0     728     774 
   Size:        728      46
 
EarI CTCTTCn'nnn_

Cuts at:      0     478     774 
   Size:        478     296
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     285     632     655     774 
   Size:        285     347      23     119

  Fragments arranged by size: 

                 347     285     119      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     493     774 
   Size:        493     281
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     448     774 
   Size:        107     341     326

  Fragments arranged by size: 

                 341     326     107
 
HaeII r_GCGC'y

Cuts at:      0      64     243     774 
   Size:         64     179     531

  Fragments arranged by size: 

                 531     179      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     296     328     501     533     774 
   Size:        296      32     173      32     241

  Fragments arranged by size: 

                 296     241     173      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      28     601     774 
   Size:         28     573     173

  Fragments arranged by size: 

                 573     173      28
 
MspA1I CmG'CkG

Cuts at:      0      72     628     669     774 
   Size:         72     556      41     105

  Fragments arranged by size: 

                 556     105      72      41
 
NaeI GCC'GGC

Cuts at:      0      11     774 
   Size:         11     763
 
NgoMIV G'CCGG_C

Cuts at:      0       9     774 
   Size:          9     765
 
NheI G'CTAG_C

Cuts at:      0      63     159     202     774 
   Size:         63      96      43     572

  Fragments arranged by size: 

                 572      96      63      43
 
NotI GC'GGCC_GC

Cuts at:      0     728     774 
   Size:        728      46
 
NspI r_CATG'y

Cuts at:      0      95     774 
   Size:         95     679
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      51     774 
   Size:         51     723
 
PspOMI G'GGCC_C

Cuts at:      0     107     447     774 
   Size:        107     340     327

  Fragments arranged by size: 

                 340     327     107
 
PvuII CAG'CTG

Cuts at:      0      72     628     774 
   Size:         72     556     146

  Fragments arranged by size: 

                 556     146      72
 
SfcI C'TryA_G

Cuts at:      0     493     774 
   Size:        493     281
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      10     774 
   Size:         10     764
 
SmaI CCC'GGG

Cuts at:      0      17     774 
   Size:         17     757
 
SmlI C'TyrA_G

Cuts at:      0      36     774 
   Size:         36     738
 
SphI G_CATG'C

Cuts at:      0      95     774 
   Size:         95     679
 
StyI C'CwwG_G

Cuts at:      0     402     774 
   Size:        402     372
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      43     774 
   Size:         43     731
 
XhoI C'TCGA_G

Cuts at:      0      36     774 
   Size:         36     738
 
XmaI C'CCGG_G

Cuts at:      0      15     774 
   Size:         15     759
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApaI     AvaI    BanII    BbvCI
     BclI     BglI     BlpI Bme1580I     BmtI     BpmI   Bpu10I    BsaAI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI   DraIII     EaeI
     EagI     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MslI
   MspA1I     NaeI   NgoMIV     NheI     NotI     NspI    PflMI   PspOMI
    PvuII     SfcI     SfiI     SmaI     SmlI     SphI     StyI    TaqII
     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI    BciVI
    BfrBI    BglII    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI     BsgI    BspEI    BspHI    BsrDI    BsrGI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI     NcoI
     NdeI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XmnI     ZraI
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