Restriction map for pF1KE0379
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AvrII       BamHI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BpmI        BpuAI       BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssSI       
Bst1107I    BstAPI      BstZ17I     CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    EcoNI       
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HpaI        KpnI        MfeI        MluI        MroI        
MseI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        ScaI        SfiI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI         2056  GTCTAC               GT^[AC][GT]AC
AlwNI        1112  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AviII        1865  TGCGCA               TGC^GCA
BalI          131  TGGCCA               TGG^CCA
BbeI         1588  GGCGCC               GGCGC^C
BbrPI        1969  CACGTG               CAC^GTG
BfaI          583  CTAG                 C^TAG
BlpI         1881  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1881  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1969  CACGTG               [CT]AC^GT[GA]
BsaBI         678  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1730  CGGCCG               CG[GA][CT]^CG
BspDI          84  ATCGAT               AT^CGAT
BsrBI        2211  GAGCGG               CCGCTC(-3/-3)
BsrFI         481  ACCGGC               [GA]^CCGG[CT]
BssHII       1214  GCGCGC               G^CGCGC
BstBI         868  TTCGAA               TT^CGAA
BstXI        1754  CCAAGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         683  AGATCC               [GA]^GATC[CT]
CelII        1881  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        481  ACCGGC               [GA]^CCGG[CT]
ClaI           84  ATCGAT               AT^CGAT
EagI         1730  CGGCCG               C^GGCCG
EclXI        1730  CGGCCG               C^GGCCG
Eco52I       1730  CGGCCG               C^GGCCG
EcoRI         391  GAATTC               G^AATTC
FspI         1865  TGCGCA               TGC^GCA
HincII        856  GTCAAC               GT[CT]^[GA]AC
HindII        856  GTCAAC               GT[CT]^[GA]AC
KasI         1588  GGCGCC               G^GCGCC
KspI         1599  CCGCGG               CCGC^GG
MaeI          583  CTAG                 C^TAG
MamI          678  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          683  AGATCC               [GA]^GATC[CT]
MluNI         131  TGGCCA               TGG^CCA
MscI          131  TGGCCA               TGG^CCA
NarI         1588  GGCGCC               GG^CGCC
NspV          868  TTCGAA               TT^CGAA
PflMI         687  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1969  CACGTG               CAC^GTG
PmlI         1969  CACGTG               CAC^GTG
PvuII        1807  CAGCTG               CAG^CTG
SacII        1599  CCGCGG               CCGC^GG
SapI         2091  GCTCTTC              GCTCTTC(1/4)
SfoI         1588  GGCGCC               GGC^GCC
SfuI          868  TTCGAA               TT^CGAA
SmaI         1724  CCCGGG               CCC^GGG
SmlI         1813  CTCAAG               C^T[CT][GA]AG
StuI          969  AGGCCT               AGG^CCT
Van91I        687  CCAGGTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1901  CCAAGCACAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         683  AGATCC               [GA]^GATC[CT]
XmaCI        1724  CCCGGG               C^CCGGG
XmaI         1724  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AatII         853  GACGTC               GACGT^C
             1321  GACGTC              
Asp700I        82  GAATCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2364  GAATAGCTTC          
AspI          706  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1330  GACAACGTC           
BanII         730  GAGCTC               G[GA]GC[CT]^C
             1825  GAGCTC              
BsmBI         786  CGTCTC               CGTCTC(1/5)
             1892  GAGACG              
BstEII       1449  GGTCACC              G^GT[ACGT]ACC
             1749  GGTGACC             
BstPI        1449  GGTCACC              G^GT[ACGT]ACC
             1749  GGTGACC             
EarI         1494  CTCTTC               CTCTTC(1/4)
             2092  CTCTTC              
EcoO65I      1449  GGTCACC              G^GT[ACGT]ACC
             1749  GGTGACC             
EcoT14I      1279  CCAAGG               C^C[AT][AT]GG
             1754  CCAAGG              
Ksp632I      1494  CTCTTC               CTCTTC(1/4)
             2092  CTCTTC              
MslI          123  CACGTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1302  CATAACTGTG          
NlaIII        241  CATG                 CATG^
             1038  CATG                
PflFI         706  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1330  GACAACGTC           
PstI         1564  CTGCAG               CTGCA^G
             1687  CTGCAG              
SacI          730  GAGCTC               GAGCT^C
             1825  GAGCTC              
SexAI        1745  ACCTGGT              A^CC[AT]GGT
             2060  ACCTGGT             
StyI         1279  CCAAGG               C^C[AT][AT]GG
             1754  CCAAGG              
Tth111I       706  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1330  GACAACGTC           
XmnI           82  GAATCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2364  GAATAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AocI        ApoI        AspHI       
AvaI        AvaII       BanI        BbvI        BcnI        
BmyI        BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        Eco81I      EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIV       PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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