Restriction map for pF1KE0513
 (Linear) (Six-base) MAPSORT of: KIEE0513.seq  Check: 6460  from: 1  to: 993



KIEE0513.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AatII G_ACGT'C

Cuts at:      0     590     993 
   Size:        590     403
 
AccI GT'mk_AC

Cuts at:      0     412     993 
   Size:        412     581
 
AclI AA'CG_TT

Cuts at:      0     476     993 
   Size:        476     517
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     865     902     993 
   Size:        541     324      37      91

  Fragments arranged by size: 

                 541     324      91      37
 
AfeI AGC'GCT

Cuts at:      0     445     993 
   Size:        445     548
 
AflIII A'CryG_T

Cuts at:      0     212     993 
   Size:        212     781
 
AhdI GACnn_n'nnGTC

Cuts at:      0     408     993 
   Size:        408     585
 
AleI CACnn'nnGTG

Cuts at:      0     322     993 
   Size:        322     671
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     199     231     993 
   Size:         27     172      32     762

  Fragments arranged by size: 

                 762     172      32      27
 
AlwNI CAG_nnn'CTG

Cuts at:      0     252     669     699     993 
   Size:        252     417      30     294

  Fragments arranged by size: 

                 417     294     252      30
 
ApoI r'AATT_y

Cuts at:      0     124     778     993 
   Size:        124     654     215

  Fragments arranged by size: 

                 654     215     124
 
AvaI C'yCGr_G

Cuts at:      0     178     993 
   Size:        178     815
 
BanII G_rGCy'C

Cuts at:      0      53      71     227     987     993 
   Size:         53      18     156     760       6

  Fragments arranged by size: 

                 760     156      53      18       6
 
BlpI GC'TnA_GC

Cuts at:      0     862     993 
   Size:        862     131
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     702     993 
   Size:        702     291
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      87     993 
   Size:         87     906
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     349     376     856     993 
   Size:        223     126      27     480     137

  Fragments arranged by size: 

                 480     223     137     126      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     993 
   Size:        247     746
 
BsaHI Gr'CG_yC

Cuts at:      0     587     973     993 
   Size:        587     386      20

  Fragments arranged by size: 

                 587     386      20
 
BsaWI w'CCGG_w

Cuts at:      0     341     615     993 
   Size:        341     274     378

  Fragments arranged by size: 

                 378     341     274
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       1      25      31     199     229     993 
   Size:          1      24       6     168      30     764

  Fragments arranged by size: 

                 764     168      30      24       6       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76      79     220     373     993 
   Size:         76       3     141     153     620

  Fragments arranged by size: 

                 620     153     141      76       3
 
BseYI C'CCAG_C

Cuts at:      0      54     993 
   Size:         54     939
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     712     751     799     993 
   Size:        403     309      39      48     194

  Fragments arranged by size: 

                 403     309     194      48      39
 
BsiHKAI G_wGCw'C

Cuts at:      0      71     185     508     913     926     993 
   Size:         71     114     323     405      13      67

  Fragments arranged by size: 

                 405     323     114      71      67      13
 
Bsp1286I G_dGCh'C

Cuts at:      0      53      71     185     227     508     913     926     987 
   Size:         53      18     114      42     281     405      13      61

Cuts at:    987     993 
   Size:          6

  Fragments arranged by size: 

                 405     281     114      61      53      42      18      13
                   6
 
BspEI T'CCGG_A

Cuts at:      0     615     993 
   Size:        615     378
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      32     104     993 
   Size:         32      72     889

  Fragments arranged by size: 

                 889      72      32
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      45     993 
   Size:         45     948
 
BtgI C'CryG_G

Cuts at:      0     978     993 
   Size:        978      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     267     993 
   Size:        267     726
 
BtsI GCAGTG_nn'

Cuts at:      0     638     993 
   Size:        638     355
 
DraIII CAC_nnn'GTG

Cuts at:      0     189     993 
   Size:        189     804
 
EaeI y'GGCC_r

Cuts at:      0     437     993 
   Size:        437     556
 
EarI CTCTTCn'nnn_

Cuts at:      0     176     993 
   Size:        176     817
 
EcoICRI GAG'CTC

Cuts at:      0      69     993 
   Size:         69     924
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     349     376     541     856     865     902     993 
   Size:        223     126      27     165     315       9      37      91

  Fragments arranged by size: 

                 315     223     165     126      91      37      27       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     205     993 
   Size:        205     788
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117     402     855     993 
   Size:        117     285     453     138

  Fragments arranged by size: 

                 453     285     138     117
 
EcoRI G'AATT_C

Cuts at:      0     778     993 
   Size:        778     215
 
EcoRV GAT'ATC

Cuts at:      0     888     993 
   Size:        888     105
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     606     993 
   Size:        606     387
 
HaeII r_GCGC'y

Cuts at:      0     262     447     835     993 
   Size:        262     185     388     158

  Fragments arranged by size: 

                 388     262     185     158
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     394     426     993 
   Size:        394      32     567

  Fragments arranged by size: 

                 567     394      32
 
HincII GTy'rAC

Cuts at:      0     660     993 
   Size:        660     333
 
MscI TGG'CCA

Cuts at:      0     439     993 
   Size:        439     554
 
MslI CAynn'nnrTG

Cuts at:      0     322     993 
   Size:        322     671
 
MspA1I CmG'CkG

Cuts at:      0      58     354     866     993 
   Size:         58     296     512     127

  Fragments arranged by size: 

                 512     296     127      58
 
NcoI C'CATG_G

Cuts at:      0     978     993 
   Size:        978      15
 
NspI r_CATG'y

Cuts at:      0     216     993 
   Size:        216     777
 
PciI A'CATG_T

Cuts at:      0     212     993 
   Size:        212     781
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     252     669     993 
   Size:        252     417     324

  Fragments arranged by size: 

                 417     324     252
 
PfoI T'CCnGG_A

Cuts at:      0     109     993 
   Size:        109     884
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      27     199     231     993 
   Size:         27     172      32     762

  Fragments arranged by size: 

                 762     172      32      27
 
PpuMI rG'GwC_Cy

Cuts at:      0     117     402     993 
   Size:        117     285     591

  Fragments arranged by size: 

                 591     285     117
 
PstI C_TGCA'G

Cuts at:      0      32     140     353     807     993 
   Size:         32     108     213     454     186

  Fragments arranged by size: 

                 454     213     186     108      32
 
PvuII CAG'CTG

Cuts at:      0     354     866     993 
   Size:        354     512     127

  Fragments arranged by size: 

                 512     354     127
 
SacI G_AGCT'C

Cuts at:      0      71     993 
   Size:         71     922
 
SfcI C'TryA_G

Cuts at:      0      28     136     349     803     993 
   Size:         28     108     213     454     190

  Fragments arranged by size: 

                 454     213     190     108      28
 
SmlI C'TyrA_G

Cuts at:      0     262     993 
   Size:        262     731
 
StyI C'CwwG_G

Cuts at:      0      38     978     993 
   Size:         38     940      15

  Fragments arranged by size: 

                 940      38      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     117     721     993 
   Size:        117     604     272

  Fragments arranged by size: 

                 604     272     117
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     378     480     715     993 
   Size:        378     102     235     278

  Fragments arranged by size: 

                 378     278     235     102
 
ZraI GAC'GTC

Cuts at:      0     588     993 
   Size:        588     405
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI     AfeI   AflIII     AhdI     AleI
     AloI    AlwNI     ApoI     AvaI    BanII     BlpI     BmrI     BplI
     BpmI    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspEI    BspMI    BstXI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII     MscI     MslI   MspA1I
     NcoI     NspI     PciI    PflMI     PfoI     PpiI    PpuMI     PstI
    PvuII     SacI     SfcI     SmlI     StyI    TaqII     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I    AflII     AgeI     AlfI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsiEI    BsiWI
     BsmI    BsmBI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI     EagI     EciI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI
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