Restriction map for pF1KB6832
 (Circular) (Six-base) MAPSORT of: pF1KB6832.seq  Check: 5823  from: 1  to: 3680



pF1KB6832  3680 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    718     718 
   Size:       3680
 
Acc65I G'GTAC_C

Cuts at:    696     696 
   Size:       3680
 
AclI AA'CG_TT

Cuts at:   3520    3520 
   Size:       3680
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    403    1505    1937    2684    2952     403 
   Size:       1102     432     747     268    1131

  Fragments arranged by size: 

                1131    1102     747     432     268
 
AflIII A'CryG_T

Cuts at:   1032    2136    3113    1032 
   Size:       1104     977    1599

  Fragments arranged by size: 

                1599    1104     977
 
AgeI A'CCGG_T

Cuts at:   2068    2068 
   Size:       3680
 
AlwNI CAG_nnn'CTG

Cuts at:    985    2552     985 
   Size:       1567    2113
 
ApaLI G'TGCA_C

Cuts at:   2450    2450 
   Size:       3680
 
ApoI r'AATT_y

Cuts at:    348     684    3033    3289     348 
   Size:        336    2349     256     739

  Fragments arranged by size: 

                2349     739     336     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3680
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3680
 
AvaI C'yCGr_G

Cuts at:    700     700 
   Size:       3680
 
BamHI G'GATC_C

Cuts at:    705     705 
   Size:       3680
 
BanI G'GyrC_C

Cuts at:    696    1110    1358    1393     696 
   Size:        414     248      35    2983

  Fragments arranged by size: 

                2983     414     248      35
 
BanII G_rGCy'C

Cuts at:    126     694    1724     126 
   Size:        568    1030    2082

  Fragments arranged by size: 

                2082    1030     568
 
BbeI G_GCGC'C

Cuts at:   1114    1362    1114 
   Size:        248    3432
 
BciVI GTATCCnnnnn_n'

Cuts at:   1578    2339    3680    1578 
   Size:        761    1341    1578

  Fragments arranged by size: 

                1578    1341     761
 
BclI T'GATC_A

Cuts at:   1200    1200 
   Size:       3680
 
BglII A'GATC_T

Cuts at:   1195    3232    1195 
   Size:       2037    1643
 
BlpI GC'TnA_GC

Cuts at:    792     792 
   Size:       3680
 
Bme1580I G_kGCm'C

Cuts at:   1305    1398    2454    1305 
   Size:         93    1056    2531

  Fragments arranged by size: 

                2531    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    229     892     973    1308    3452     229 
   Size:        663      81     335    2144     457

  Fragments arranged by size: 

                2144     663     457     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2047    2047 
   Size:       3680
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    629     856    1735    2227    2525     629 
   Size:        227     879     492     298    1784

  Fragments arranged by size: 

                1784     879     492     298     227
 
BsaI GGTCTCn'nnnn_

Cuts at:   3384    3384 
   Size:       3680
 
BsaAI yAC'GTr

Cuts at:   1033    1033 
   Size:       3680
 
BsaBI GATnn'nnATC

Cuts at:      6    1194    3231       6 
   Size:       1188    2037     455

  Fragments arranged by size: 

                2037    1188     455
 
BsaHI Gr'CG_yC

Cuts at:   1111    1359    2061    3557    1111 
   Size:        248     702    1496    1234

  Fragments arranged by size: 

                1496    1234     702     248
 
BsaWI w'CCGG_w

Cuts at:    398     869    1390    2068    2342    2489    2900     398 
   Size:        471     521     678     274     147     411    1178

  Fragments arranged by size: 

                1178     678     521     471     411     274     147
 
BseYI C'CCAG_C

Cuts at:    193    1105    1863    2440    2936     193 
   Size:        912     758     577     496     937

  Fragments arranged by size: 

                 937     912     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    446     446 
   Size:       3680
 
BsiEI CG_ry'CG

Cuts at:    104    1222    1268    2476     104 
   Size:       1118      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1118      46
 
BsiHKAI G_wGCw'C

Cuts at:    126     694    1472    1662    2454     126 
   Size:        568     778     190     792    1352

  Fragments arranged by size: 

                1352     792     778     568     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    154     154 
   Size:       3680
 
Bsp1286I G_dGCh'C

Cuts at:    126     694    1305    1398    1472    1662    1724    2454     126 
   Size:        568     611      93      74     190      62     730    1352

  Fragments arranged by size: 

                1352     730     611     568     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2900    2900 
   Size:       3680
 
BspHI T'CATG_A

Cuts at:    182     450    2856     182 
   Size:        268    2406    1006

  Fragments arranged by size: 

                2406    1006     268
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    418     730     944    1246    1627     418 
   Size:        312     214     302     381    2471

  Fragments arranged by size: 

                2471     381     312     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1972    2026    3510    3675      50 
   Size:       1922      54    1484     165      55

  Fragments arranged by size: 

                1922    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1592    1592 
   Size:       3680
 
BsrFI r'CCGG_y

Cuts at:   1678    1859    2068    1678 
   Size:        181     209    3290

  Fragments arranged by size: 

                3290     209     181
 
BssSI C'ACGA_G

Cuts at:   1951    2309    1951 
   Size:        358    3322
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1057    1057 
   Size:       3680
 
BstBI TT'CG_AA

Cuts at:   2041    2041 
   Size:       3680
 
BstEII G'GTnAC_C

Cuts at:    204     204 
   Size:       3680
 
BstYI r'GATC_y

Cuts at:      1     705    1172    1195    1530    1776    2788    2874    2886 
   Size:        704     467      23     335     246    1012      86      12

Cuts at:   2886    3232       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     704     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3680
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1630    1811    1974    1630 
   Size:        181     163    3336

  Fragments arranged by size: 

                3336     181     163
 
BtsI GCAGTG_nn'

Cuts at:    923     923 
   Size:       3680
 
DraI TTT'AAA

Cuts at:    679     679 
   Size:       3680
 
DrdI GACnn_nn'nnGTC

Cuts at:   1386    2244    1386 
   Size:        858    2822
 
EaeI y'GGCC_r

Cuts at:   1265    1439    1830    1857    3202    1265 
   Size:        174     391      27    1345    1743

  Fragments arranged by size: 

                1743    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1265    1265 
   Size:       3680
 
EarI CTCTTCn'nnn_

Cuts at:   1703    1913    1703 
   Size:        210    3470
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1888    2198    2344    3490    1888 
   Size:        310     146    1146    2078

  Fragments arranged by size: 

                2078    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    124     692     124 
   Size:        568    3112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    403    1505    1937    2684    2952     403 
   Size:       1102     432     747     268    1131

  Fragments arranged by size: 

                1131    1102     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    405     430     577     819     405 
   Size:         25     147     242    3266

  Fragments arranged by size: 

                3266     242     147      25
 
EcoRI G'AATT_C

Cuts at:    684     684 
   Size:       3680
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1699    1699 
   Size:       3680
 
FspI TGC'GCA

Cuts at:   1461    1461 
   Size:       3680
 
HaeII r_GCGC'y

Cuts at:   1114    1362    2384    3363    1114 
   Size:        248    1022     979    1431

  Fragments arranged by size: 

                1431    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    113     145    1522    1552    1554    1584     113 
   Size:         32    1377      30       2      30    2209

  Fragments arranged by size: 

                2209    1377      32      30      30       2
 
HincII GTy'rAC

Cuts at:    719     719 
   Size:       3680
 
KasI G'GCGC_C

Cuts at:   1110    1358    1110 
   Size:        248    3432
 
KpnI G_GTAC'C

Cuts at:    700     700 
   Size:       3680
 
MfeI C'AATT_G

Cuts at:    608     608 
   Size:       3680
 
MluI A'CGCG_T

Cuts at:   3113    3113 
   Size:       3680
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2351    2535    2351 
   Size:        184    3496
 
MscI TGG'CCA

Cuts at:   1441    1441 
   Size:       3680
 
MslI CAynn'nnrTG

Cuts at:   1669    1796    1669 
   Size:        127    3553
 
MspA1I CmG'CkG

Cuts at:    791    1105    1465    2478    2723     791 
   Size:        314     360    1013     245    1748

  Fragments arranged by size: 

                1748    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1861    1861 
   Size:       3680
 
NarI GG'CG_CC

Cuts at:   1111    1359    1111 
   Size:        248    3432
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3680
 
NdeI CA'TA_TG

Cuts at:    306     306 
   Size:       3680
 
NgoMIV G'CCGG_C

Cuts at:   1859    1859 
   Size:       3680
 
NspI r_CATG'y

Cuts at:    733    1233    2140     733 
   Size:        500     907    2273

  Fragments arranged by size: 

                2273     907     500
 
PciI A'CATG_T

Cuts at:   2136    2136 
   Size:       3680
 
PmeI GTTT'AAAC

Cuts at:    679     679 
   Size:       3680
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2819    2851    2819 
   Size:         32    3648
 
PpuMI rG'GwC_Cy

Cuts at:    430     430 
   Size:       3680
 
PstI C_TGCA'G

Cuts at:    727    1412     727 
   Size:        685    2995
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3680
 
PvuII CAG'CTG

Cuts at:   1105    1465    1105 
   Size:        360    3320
 
RsrII CG'GwC_CG

Cuts at:   1875    1875 
   Size:       3680
 
SacI G_AGCT'C

Cuts at:    126     694     126 
   Size:        568    3112
 
SalI G'TCGA_C

Cuts at:    717     717 
   Size:       3680
 
SapI GCTCTTCn'nnn_

Cuts at:   1703    1913    1703 
   Size:        210    3470
 
SbfI CC_TGCA'GG

Cuts at:    727     727 
   Size:       3680
 
ScaI AGT'ACT

Cuts at:   3197    3197 
   Size:       3680
 
SfcI C'TryA_G

Cuts at:     33     173     723    1408    2401    2592      33 
   Size:        140     550     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     550     191     140
 
SfoI GGC'GCC

Cuts at:   1112    1360    1112 
   Size:        248    3432
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3680
 
SmaI CCC'GGG

Cuts at:    702     702 
   Size:       3680
 
SmlI C'TyrA_G

Cuts at:    644     835    1750    2242    2504     644 
   Size:        191     915     492     262    1820

  Fragments arranged by size: 

                1820     915     492     262     191
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3680
 
SphI G_CATG'C

Cuts at:    733    1233     733 
   Size:        500    3180
 
StuI AGG'CCT

Cuts at:    218     218 
   Size:       3680
 
StyI C'CwwG_G

Cuts at:    107     814    1168     107 
   Size:        707     354    2619

  Fragments arranged by size: 

                2619     707     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1263    1396    1855    2064    1263 
   Size:        133     459     209    2879

  Fragments arranged by size: 

                2879     459     209     133
 
TatI w'GTAC_w

Cuts at:    237    3195     237 
   Size:       2958     722
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3197    3229    3197 
   Size:         32    3648
 
Tth111I GACn'n_nGTC

Cuts at:   1477    1477 
   Size:       3680
 
XbaI T'CTAG_A

Cuts at:    711     711 
   Size:       3680
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    123    1175     123 
   Size:       1052    2628
 
XmaI C'CCGG_G

Cuts at:    700     700 
   Size:       3680
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PciI     PmeI
     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI
     BsmI    BsrGI   BssHII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
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