Restriction map for pF1KB7211
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BanI        BbeI        BbrPI       BcgI        
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       BspEI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     CelII       
CfoI        Cfr10I      CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MluI        MroI        MseI        
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AciI          255  GCGG                 CCGC(-3/-1)
AflIII        718  ACATGT               A^C[GA][CT]GT
Asp700I       906  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         415  GAGCTC               G[AT]GC[AT]^C
AvaII         681  GGTCC                G^G[AT]CC
BalI          768  TGGCCA               TGG^CCA
BamHI         698  GGATCC               G^GATCC
BbvCI         186  CCTCAGC              CCTCAGC(-5/-2)
BciVI         638  GTATCC               GTATCC(6/5)
BcnI          491  CCCGG                CC^[GC]GG
BsiHKAI       415  GAGCTC               G[AT]GC[AT]^C
BsmAI         744  GTCTC                GTCTC(1/5)
BsmI          286  GAATGC               GAATGC(1/-1)
Bsp1407I      830  TGTACA               T^GTACA
BspDI         270  ATCGAT               AT^CGAT
BspHI         404  TCATGA               T^CATGA
BspLU11I      718  ACATGT               A^CATGT
BspMI         125  ACCTGC               ACCTGC(4/8)
BsrGI         830  TGTACA               T^GTACA
BsrI          771  CCAGT                ACTGG(1/-1)
BstXI         110  CCACGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         698  GGATCC               [GA]^GATC[CT]
ClaI          270  ATCGAT               AT^CGAT
DsaI          110  CCACGG               C^C[GA][CT]GG
EcoNI         276  CCTCTATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         571  GATATC               GAT^ATC
EcoT14I       900  CCTTGG               C^C[AT][AT]GG
EcoT22I       288  ATGCAT               ATGCA^T
MflI          698  GGATCC               [GA]^GATC[CT]
MluNI         768  TGGCCA               TGG^CCA
MscI          768  TGGCCA               TGG^CCA
MspA1I        148  CAGCTG               C[AC]G^C[GT]G
NciI          491  CCCGG                CC^[GC]GG
NsiI          288  ATGCAT               ATGCA^T
PleI          154  GACTC                GAGTC(4/5)
PpuMI         680  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         148  CAGCTG               CAG^CTG
RcaI          404  TCATGA               T^CATGA
SacI          415  GAGCTC               GAGCT^C
SapI          412  GAAGAGC              GCTCTTC(1/4)
SfaNI         240  GATGC                GCATC(5/9)
SfcI          867  CTACAG               C^T[GA][CT]AG
SmlI          553  CTCAAG               C^T[CT][GA]AG
SspBI         830  TGTACA               T^GTACA
SspI          762  AATATT               AAT^ATT
StyI          900  CCTTGG               C^C[AT][AT]GG
TaqI          271  TCGA                 T^CGA
TfiI          447  GATTC                G^A[AT]TC
TthHB8I       271  TCGA                 T^CGA
XhoII         698  GGATCC               [GA]^GATC[CT]
XmnI          906  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI          675  CCTGAGG              CC^T[ACGT]AGG
              873  CCTTAGG             
BanII           3  GAGCCC               G[GA]GC[CT]^C
              415  GAGCTC              
BbsI          424  GTCTTC               GAAGAC(2/6)
              529  GTCTTC              
BmyI            3  GAGCCC               G[AGT]GC[ACT]^C
              415  GAGCTC              
BpuAI         424  GTCTTC               GAAGAC(2/6)
              529  GTCTTC              
Bsp1286I        3  GAGCCC               G[AGT]GC[ACT]^C
              415  GAGCTC              
Bsu36I        675  CCTGAGG              CC^T[ACGT]AGG
              873  CCTTAGG             
Cac8I          69  GCAGGC               GC[ACGT]^[ACGT]GC
              460  GCCAGC              
DpnII         273  GATC                 ^GATC
              699  GATC                
DraII         680  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              896  AGGCCCT             
DrdI          489  GACCCGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              805  GACAAGGATGTC        
EaeI           58  CGGCCA               [CT]^GGCC[GA]
              768  TGGCCA              
EarI          910  CTCTTC               CTCTTC(1/4)
              412  GAAGAG              
Eco81I        675  CCTGAGG              CC^T[ACGT]AGG
              873  CCTTAGG             
EcoO109I      680  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              896  AGGCCCT             
HapII          57  CCGG                 C^CGG
              492  CCGG                
HinfI         154  GACTC                G^A[ACGT]TC
              447  GATTC               
HpaII          57  CCGG                 C^CGG
              492  CCGG                
Ksp632I       910  CTCTTC               CTCTTC(1/4)
              412  GAAGAG              
MboI          273  GATC                 ^GATC
              699  GATC                
MspI           57  CCGG                 C^CGG
              492  CCGG                
NdeII         273  GATC                 ^GATC
              699  GATC                
Sau3AI        273  GATC                 ^GATC
              699  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AfaI        AluI        AlwI        BbvI        BsaJI       
BsiYI       BslI        BsmFI       BssKI       BstF5I      
BstNI       Cfr13I      DdeI        EcoRII      Fnu4HI      
FokI        HaeIII      HphI        ItaI        MaeIII      
MboII       MnlI        MslI        MvaI        MwoI        
NdeI        NlaIII      NlaIV       NspI        RsaI        
Sau96I      ScrFI       TseI        Tsp45I      TspRI       
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