Restriction map for pF1KB7211
 (Linear) (Six-base) MAPSORT of: KIBB7211.seq  Check: 9939  from: 1  to: 939



KIBB7211.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     897     939 
   Size:        897      42
 
AflIII A'CryG_T

Cuts at:      0     718     939 
   Size:        718     221
 
BamHI G'GATC_C

Cuts at:      0     698     939 
   Size:        698     241
 
BanII G_rGCy'C

Cuts at:      0       7     419     939 
   Size:          7     412     520

  Fragments arranged by size: 

                 520     412       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     417     522     939 
   Size:        417     105     417

  Fragments arranged by size: 

                 417     417     105
 
BbvCI CC'TCA_GC

Cuts at:      0     187     939 
   Size:        187     752
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     649     939 
   Size:        649     290
 
BfrBI ATG'CAT

Cuts at:      0     290     939 
   Size:        290     649
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     939 
   Size:        187     752
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538     939 
   Size:        538     401
 
BseYI C'CCAG_C

Cuts at:      0     117     939 
   Size:        117     822
 
BsiHKAI G_wGCw'C

Cuts at:      0     419     939 
   Size:        419     520
 
BsmI GAATG_Cn'

Cuts at:      0     292     939 
   Size:        292     647
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     419     939 
   Size:          7     412     520

  Fragments arranged by size: 

                 520     412       7
 
BspHI T'CATG_A

Cuts at:      0     404     939 
   Size:        404     535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     134     939 
   Size:        134     805
 
BsrGI T'GTAC_A

Cuts at:      0     830     939 
   Size:        830     109
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     117     939 
   Size:        117     822
 
BstYI r'GATC_y

Cuts at:      0     698     939 
   Size:        698     241
 
Bsu36I CC'TnA_GG

Cuts at:      0     676     874     939 
   Size:        676     198      65

  Fragments arranged by size: 

                 676     198      65
 
BtgI C'CryG_G

Cuts at:      0     110     939 
   Size:        110     829
 
ClaI AT'CG_AT

Cuts at:      0     271     939 
   Size:        271     668
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     495     811     939 
   Size:        495     316     128

  Fragments arranged by size: 

                 495     316     128
 
EaeI y'GGCC_r

Cuts at:      0      58     768     939 
   Size:         58     710     171

  Fragments arranged by size: 

                 710     171      58
 
EarI CTCTTCn'nnn_

Cuts at:      0     407     916     939 
   Size:        407     509      23

  Fragments arranged by size: 

                 509     407      23
 
EcoICRI GAG'CTC

Cuts at:      0     417     939 
   Size:        417     522
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     897     939 
   Size:        897      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     280     939 
   Size:        280     659
 
EcoO109I rG'GnC_Cy

Cuts at:      0     681     897     939 
   Size:        681     216      42

  Fragments arranged by size: 

                 681     216      42
 
EcoRV GAT'ATC

Cuts at:      0     573     939 
   Size:        573     366
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     916     939 
   Size:        916      23
 
MscI TGG'CCA

Cuts at:      0     770     939 
   Size:        770     169
 
MslI CAynn'nnrTG

Cuts at:      0     172     633     847     939 
   Size:        172     461     214      92

  Fragments arranged by size: 

                 461     214     172      92
 
MspA1I CmG'CkG

Cuts at:      0     150     939 
   Size:        150     789
 
NdeI CA'TA_TG

Cuts at:      0     357     376     651     939 
   Size:        357      19     275     288

  Fragments arranged by size: 

                 357     288     275      19
 
NsiI A_TGCA'T

Cuts at:      0     292     939 
   Size:        292     647
 
NspI r_CATG'y

Cuts at:      0     102     255     722     939 
   Size:        102     153     467     217

  Fragments arranged by size: 

                 467     217     153     102
 
PciI A'CATG_T

Cuts at:      0     718     939 
   Size:        718     221
 
PpuMI rG'GwC_Cy

Cuts at:      0     681     939 
   Size:        681     258
 
PvuII CAG'CTG

Cuts at:      0     150     939 
   Size:        150     789
 
SacI G_AGCT'C

Cuts at:      0     419     939 
   Size:        419     520
 
SanDI GG'GwC_CC

Cuts at:      0     681     939 
   Size:        681     258
 
SapI GCTCTTCn'nnn_

Cuts at:      0     407     939 
   Size:        407     532
 
SfcI C'TryA_G

Cuts at:      0     867     939 
   Size:        867      72
 
SmlI C'TyrA_G

Cuts at:      0     553     939 
   Size:        553     386
 
SspI AAT'ATT

Cuts at:      0     764     939 
   Size:        764     175
 
StyI C'CwwG_G

Cuts at:      0     900     939 
   Size:        900      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     377     861     939 
   Size:        377     484      78

  Fragments arranged by size: 

                 484     377      78
 
TatI w'GTAC_w

Cuts at:      0     176     263     830     939 
   Size:        176      87     567     109

  Fragments arranged by size: 

                 567     176     109      87
 
XmnI GAAnn'nnTTC

Cuts at:      0     910     939 
   Size:        910      29
 

 Enzymes that do cut:

     AcuI   AflIII    BamHI    BanII     BbsI    BbvCI    BciVI    BfrBI
   Bpu10I    BpuEI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrGI    BstXI    BstYI   Bsu36I     BtgI     ClaI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     MscI
     MslI   MspA1I     NdeI     NsiI     NspI     PciI    PpuMI    PvuII
     SacI    SanDI     SapI     SfcI     SmlI     SspI     StyI    TaqII
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     BtsI    CspCI     DraI   DraIII     EagI     EciI    EcoRI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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