Restriction map for pF1KB8581
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvrII       
BalI        BbrPI       BbvCI       BciVI       BfrI        
BglI        BlnI        BsaAI       BsaBI       BsaI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
EcoT22I     FseI        HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfaNI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccIII       1550  TCCGGA               T^CCGGA
AflIII       1376  ACACGT               A^C[GA][CT]GT
AlwNI         917  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AviII        1437  TGCGCA               TGC^GCA
BamHI         902  GGATCC               G^GATCC
BbsI         1210  GAAGAC               GAAGAC(2/6)
BcgI         1088  GCACGCTGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1007  TGATCA               T^GATCA
BfaI         1030  CTAG                 C^TAG
BglII        1418  AGATCT               A^GATCT
BpuAI        1210  GAAGAC               GAAGAC(2/6)
BseAI        1550  TCCGGA               T^CCGGA
BsgI         1123  CTGCAC               GTGCAG(16/14)
BspEI        1550  TCCGGA               T^CCGGA
BstEII       1503  GGTGACC              G^GT[ACGT]ACC
BstPI        1503  GGTGACC              G^GT[ACGT]ACC
BstXI         437  CCACCTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          879  TTTAAA               TTT^AAA
EarI         1158  GAAGAG               CTCTTC(1/4)
EcoO65I      1503  GGTGACC              G^GT[ACGT]ACC
EcoRI         531  GAATTC               G^AATTC
FbaI         1007  TGATCA               T^GATCA
FspI         1437  TGCGCA               TGC^GCA
HincII       1072  GTCAAC               GT[CT]^[GA]AC
HindII       1072  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1158  GAAGAG               CTCTTC(1/4)
MaeI         1030  CTAG                 C^TAG
MroI         1550  TCCGGA               T^CCGGA
NaeI         1523  GCCGGC               GCC^GGC
NcoI          320  CCATGG               C^CATGG
NgoMIV       1523  GCCGGC               G^CCGGC
SacI         1174  GAGCTC               GAGCT^C
ScaI          646  AGTACT               AGT^ACT


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          531  GAATTC               [GA]^AATT[CT]
              876  GAATTT              
AcyI          739  GGCGCC               G[GA]^CG[CT]C
              891  GGCGCC              
ApoI          531  GAATTC               [GA]^AATT[CT]
              876  GAATTT              
BbeI          739  GGCGCC               GGCGC^C
              891  GGCGCC              
BcnI          899  CCGGG                CC^[GC]GG
             1382  CCGGG               
BlpI          521  GCTCAGC              GC^T[ACGT]AGC
             1218  GCTGAGC             
Bpu1102I      521  GCTCAGC              GC^T[ACGT]AGC
             1218  GCTGAGC             
BsaHI         739  GGCGCC               G[GA]^CG[CT]C
              891  GGCGCC              
BsaWI         505  TCCGGT               [AT]^CCGG[AT]
             1550  TCCGGA              
CelII         521  GCTCAGC              GC^T[ACGT]AGC
             1218  GCTGAGC             
DsaI          320  CCATGG               C^C[GA][CT]GG
              545  CCACGG              
EaeI          200  CGGCCA               [CT]^GGCC[GA]
              575  CGGCCA              
EcoNI          82  CCTGCTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1053  CCTCCAGCAGG         
EcoT14I       320  CCATGG               C^C[AT][AT]GG
             1508  CCAAGG              
HaeII         739  GGCGCC               [GA]GCGC^[CT]
              891  GGCGCC              
HgaI         1321  GACGC                GACGC(5/10)
              667  GCGTC               
Hin1I         739  GGCGCC               G[GA]^CG[CT]C
              891  GGCGCC              
KasI          739  GGCGCC               G^GCGCC
              891  GGCGCC              
MboII        1158  GAAGA                GAAGA(8/7)
             1210  GAAGA               
NarI          739  GGCGCC               GG^CGCC
              891  GGCGCC              
NciI          899  CCGGG                CC^[GC]GG
             1382  CCGGG               
SfoI          739  GGCGCC               GGC^GCC
              891  GGCGCC              
StyI          320  CCATGG               C^C[AT][AT]GG
             1508  CCAAGG              
XcmI          311  CCACCCCAGCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              434  CCACCACCTCTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
ApaI        AspHI       AvaII       BanI        BanII       
BbvI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        SmlI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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