Restriction map for pF1KB7383
 (Linear) (Six-base) MAPSORT of: KIBB7383.seq  Check: 7864  from: 1  to: 1050



KIBB7383.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     477     873    1050 
   Size:        477     396     177

  Fragments arranged by size: 

                 477     396     177
 
AccI GT'mk_AC

Cuts at:      0     282    1050 
   Size:        282     768
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     659    1050 
   Size:        223     436     391

  Fragments arranged by size: 

                 436     391     223
 
ApoI r'AATT_y

Cuts at:      0     157    1050 
   Size:        157     893
 
AvaI C'yCGr_G

Cuts at:      0     925     994    1050 
   Size:        925      69      56

  Fragments arranged by size: 

                 925      69      56
 
BamHI G'GATC_C

Cuts at:      0     238    1050 
   Size:        238     812
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     211     317     427    1050 
   Size:        211     106     110     623

  Fragments arranged by size: 

                 623     211     110     106
 
BbvCI CC'TCA_GC

Cuts at:      0     828     840     897     954     966    1025    1050 
   Size:        828      12      57      57      12      59      25

  Fragments arranged by size: 

                 828      59      57      57      25      12      12
 
BglI GCCn_nnn'nGGC

Cuts at:      0     690    1050 
   Size:        690     360
 
BlpI GC'TnA_GC

Cuts at:      0      28    1050 
   Size:         28    1022
 
Bpu10I CC'TnA_GC

Cuts at:      0      12     781     828     840     897     954     966    1025 
   Size:         12     769      47      12      57      57      12      59

Cuts at:   1025    1050 
   Size:         25

  Fragments arranged by size: 

                 769      59      57      57      47      25      12      12
                  12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772     841     898     967    1026    1050 
   Size:        772      69      57      69      59      24

  Fragments arranged by size: 

                 772      69      69      59      57      24
 
BsaBI GATnn'nnATC

Cuts at:      0     851     908     977    1036    1050 
   Size:        851      57      69      59      14

  Fragments arranged by size: 

                 851      69      59      57      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     734    1050 
   Size:        734     316
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159    1050 
   Size:        159     891
 
BsiHKAI G_wGCw'C

Cuts at:      0     470    1050 
   Size:        470     580
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     224    1050 
   Size:        224     826
 
Bsp1286I G_dGCh'C

Cuts at:      0     470    1050 
   Size:        470     580
 
BspHI T'CATG_A

Cuts at:      0     396    1050 
   Size:        396     654
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     477     873    1050 
   Size:        477     396     177

  Fragments arranged by size: 

                 477     396     177
 
BsrBI CCG'CTC

Cuts at:      0      32    1050 
   Size:         32    1018
 
BsrGI T'GTAC_A

Cuts at:      0     249    1050 
   Size:        249     801
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     570    1050 
   Size:        570     480
 
BstYI r'GATC_y

Cuts at:      0     238    1050 
   Size:        238     812
 
BtgI C'CryG_G

Cuts at:      0     242    1050 
   Size:        242     808
 
BtsI GCAGTG_nn'

Cuts at:      0     662    1050 
   Size:        662     388
 
DraI TTT'AAA

Cuts at:      0     716    1050 
   Size:        716     334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     659    1050 
   Size:        223     436     391

  Fragments arranged by size: 

                 436     391     223
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     228    1050 
   Size:        228     822
 
FspI TGC'GCA

Cuts at:      0     563    1050 
   Size:        563     487
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     344     376     590     622    1050 
   Size:        344      32     214      32     428

  Fragments arranged by size: 

                 428     344     214      32      32
 
HincII GTy'rAC

Cuts at:      0     390    1050 
   Size:        390     660
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     772    1050 
   Size:        296     476     278

  Fragments arranged by size: 

                 476     296     278
 
MslI CAynn'nnrTG

Cuts at:      0     136     541     547    1050 
   Size:        136     405       6     503

  Fragments arranged by size: 

                 503     405     136       6
 
MspA1I CmG'CkG

Cuts at:      0     706     844     901     970    1029    1050 
   Size:        706     138      57      69      59      21

  Fragments arranged by size: 

                 706     138      69      59      57      21
 
PfoI T'CCnGG_A

Cuts at:      0     305    1050 
   Size:        305     745
 
PshAI GACnn'nnGTC

Cuts at:      0      86    1050 
   Size:         86     964
 
PsiI TTA'TAA

Cuts at:      0     155    1050 
   Size:        155     895
 
PvuII CAG'CTG

Cuts at:      0     706    1050 
   Size:        706     344
 
SfcI C'TryA_G

Cuts at:      0     594    1050 
   Size:        594     456
 
SmlI C'TyrA_G

Cuts at:      0     787     856     913     982    1041    1050 
   Size:        787      69      57      69      59       9

  Fragments arranged by size: 

                 787      69      69      59      57       9
 
StyI C'CwwG_G

Cuts at:      0     118    1050 
   Size:        118     932
 
TatI w'GTAC_w

Cuts at:      0     249    1050 
   Size:        249     801
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     804     836     930     962    1001    1033    1050 
   Size:        804      32      94      32      39      32      17

  Fragments arranged by size: 

                 804      94      39      32      32      32      17
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      91    1050 
   Size:         91     959
 
XmnI GAAnn'nnTTC

Cuts at:      0     551    1050 
   Size:        551     499
 

 Enzymes that do cut:

     AarI     AccI     AcuI     ApoI     AvaI    BamHI     BbsI    BbvCI
     BglI     BlpI   Bpu10I    BpuEI    BsaBI    BseRI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrGI   BstAPI    BstYI
     BtgI     BtsI     DraI  Eco57MI     FalI     FspI    Hin4I   HincII
     MmeI     MslI   MspA1I     PfoI    PshAI     PsiI    PvuII     SfcI
     SmlI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI    BanII     BbeI     BcgI    BciVI
     BclI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
    BsiWI     BsmI    BspEI    BsrDI    BsrFI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmaI     ZraI
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