Restriction map for pF1KB6488
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AflII       AflIII      AgeI        AhdI        
AocI        ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbvCI       BciVI       BclI        BfrI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       Bst1107I    
BstBI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tth111I     XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcyI          135  GACGCC               G[GA]^CG[CT]C
Alw44I        390  GTGCAC               G^TGCAC
AlwI          512  GGATC                GGATC(4/5)
Aor51HI        98  AGCGCT               AGC^GCT
ApaLI         390  GTGCAC               G^TGCAC
AvrII         582  CCTAGG               C^CTAGG
BanI          486  GGTGCC               G^G[CT][GA]CC
BbsI         1050  GAAGAC               GAAGAC(2/6)
BcgI           34  CGAAAGCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          583  CTAG                 C^TAG
BglI          206  GCCAGCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         228  AGATCT               A^GATCT
BlnI          582  CCTAGG               C^CTAGG
BpuAI        1050  GAAGAC               GAAGAC(2/6)
BsaHI         135  GACGCC               G[GA]^CG[CT]C
BseRI         529  GAGGAG               GAGGAG(10/8)
BssHII        162  GCGCGC               G^CGCGC
BssSI          18  CACGAG               CACGAG(-5/-1)
BstAPI          3  GCAGAGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        681  GGTCACC              G^GT[ACGT]ACC
BstPI         681  GGTCACC              G^GT[ACGT]ACC
DraII         985  GGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII        630  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          601  GACTCAAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          891  TGGCCG               [CT]^GGCC[GA]
Eco47III       98  AGCGCT               AGC^GCT
EcoNI          75  CCTGGGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      985  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       681  GGTCACC              G^GT[ACGT]ACC
EcoT14I       582  CCTAGG               C^C[AT][AT]GG
HaeII          98  AGCGCT               [GA]GCGC^[CT]
HgaI          135  GACGC                GACGC(5/10)
Hin1I         135  GACGCC               G[GA]^CG[CT]C
HincII        610  GTCAAC               GT[CT]^[GA]AC
HindII        610  GTCAAC               GT[CT]^[GA]AC
HphI          683  TCACC                GGTGA(8/7)
MaeI          583  CTAG                 C^TAG
MboII        1050  GAAGA                GAAGA(8/7)
NspI          643  GCATGC               [GA]CATG^[CT]
PpuMI         985  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         433  CAGCTG               CAG^CTG
SmlI          603  CTCAAG               C^T[CT][GA]AG
SphI          643  GCATGC               GCATG^C
StyI          582  CCTAGG               C^C[AT][AT]GG
TaqI          335  TCGA                 T^CGA
TfiI          535  GATTC                G^A[AT]TC
TthHB8I       335  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI          364  AAATTT               [GA]^AATT[CT]
              382  AAATTC              
AlwNI         867  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1036  CAGGAGCTG           
ApoI          364  AAATTT               [GA]^AATT[CT]
              382  AAATTC              
AvaII         279  GGACC                G^G[AT]CC
              986  GGACC               
BsmFI          78  GGGAC                GGGAC(10/14)
              985  GGGAC               
BstYI         228  AGATCT               [GA]^GATC[CT]
              512  GGATCT              
FauI          203  CCCGC                CCCGC(4/6)
              425  CCCGC               
MflI          228  AGATCT               [GA]^GATC[CT]
              512  GGATCT              
SfaNI         708  GATGC                GCATC(5/9)
              299  GCATC               
StuI          546  AGGCCT               AGG^CCT
             1025  AGGCCT              
Tsp509I       365  AATT                 ^AATT
              383  AATT                
XhoII         228  AGATCT               [GA]^GATC[CT]
              512  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaI        
BbvI        BcnI        BmyI        BpmI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MaeIII      MboI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PstI        Sau3AI      Sau96I      
ScrFI       SfcI        SmaI        TseI        Tsp45I      
TspRI       Van91I      XmaCI       XmaI        
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