Restriction map for pF1KB6488
 (Linear) (Six-base) MAPSORT of: KIBB6488.seq  Check: 6807  from: 1  to: 1071



KIBB6488.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     403     833    1071 
   Size:        403     430     238

  Fragments arranged by size: 

                 430     403     238
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1069    1071 
   Size:       1069       2
 
AfeI AGC'GCT

Cuts at:      0     100    1071 
   Size:        100     971
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     865    1071 
   Size:        865     206
 
AlwNI CAG_nnn'CTG

Cuts at:      0     872    1041    1071 
   Size:        872     169      30

  Fragments arranged by size: 

                 872     169      30
 
ApaLI G'TGCA_C

Cuts at:      0     390    1071 
   Size:        390     681
 
ApoI r'AATT_y

Cuts at:      0     364     382    1071 
   Size:        364      18     689

  Fragments arranged by size: 

                 689     364      18
 
AvaI C'yCGr_G

Cuts at:      0     117     154     677    1071 
   Size:        117      37     523     394

  Fragments arranged by size: 

                 523     394     117      37
 
AvrII C'CTAG_G

Cuts at:      0     582    1071 
   Size:        582     489
 
BanI G'GyrC_C

Cuts at:      0     486    1071 
   Size:        486     585
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1057    1071 
   Size:       1057      14
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      23      57    1071 
   Size:         23      34    1014

  Fragments arranged by size: 

                1014      34      23
 
BglI GCCn_nnn'nGGC

Cuts at:      0     212    1071 
   Size:        212     859
 
BglII A'GATC_T

Cuts at:      0     228    1071 
   Size:        228     843
 
Bme1580I G_kGCm'C

Cuts at:      0     118     130     204     394     425    1071 
   Size:        118      12      74     190      31     646

  Fragments arranged by size: 

                 646     190     118      74      31      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     105     550    1071 
   Size:        105     445     521

  Fragments arranged by size: 

                 521     445     105
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     240    1071 
   Size:        240     831
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     512    1018    1063    1071 
   Size:        253     259     506      45       8

  Fragments arranged by size: 

                 506     259     253      45       8
 
Bpu10I CC'TnA_GC

Cuts at:      0     452    1071 
   Size:        452     619
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     588    1071 
   Size:        588     483
 
BsaHI Gr'CG_yC

Cuts at:      0     136    1071 
   Size:        136     935
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     578     608    1071 
   Size:        578      30     463

  Fragments arranged by size: 

                 578     463      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     544    1071 
   Size:        544     527
 
BseYI C'CCAG_C

Cuts at:      0     283     465    1071 
   Size:        283     182     606

  Fragments arranged by size: 

                 606     283     182
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     288     825     835     885     975    1071 
   Size:         31     257     537      10      50      90      96

  Fragments arranged by size: 

                 537     257      96      90      50      31      10
 
BsiHKAI G_wGCw'C

Cuts at:      0      19     394     946    1071 
   Size:         19     375     552     125

  Fragments arranged by size: 

                 552     375     125      19
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     118     130     204     394     425     946    1071 
   Size:         19      99      12      74     190      31     521     125

  Fragments arranged by size: 

                 521     190     125      99      74      31      19      12
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     154     403     833     997    1071 
   Size:        154     249     430     164      74

  Fragments arranged by size: 

                 430     249     164     154      74
 
BssHII G'CGCG_C

Cuts at:      0     162    1071 
   Size:        162     909
 
BssSI C'ACGA_G

Cuts at:      0      18    1071 
   Size:         18    1053
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0       9    1071 
   Size:          9    1062
 
BstEII G'GTnAC_C

Cuts at:      0     681    1071 
   Size:        681     390
 
BstYI r'GATC_y

Cuts at:      0     228     512    1071 
   Size:        228     284     559

  Fragments arranged by size: 

                 559     284     228
 
BtsI GCAGTG_nn'

Cuts at:      0      46     258     360     891    1071 
   Size:         46     212     102     531     180

  Fragments arranged by size: 

                 531     212     180     102      46
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     639     674    1071 
   Size:        639      35     397

  Fragments arranged by size: 

                 639     397      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     635    1071 
   Size:        635     436
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     607    1071 
   Size:        607     464
 
EaeI y'GGCC_r

Cuts at:      0     891    1071 
   Size:        891     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     512    1018    1063    1069    1071 
   Size:        253     259     506      45       6       2

  Fragments arranged by size: 

                 506     259     253      45       6       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      79    1071 
   Size:         79     992
 
EcoO109I rG'GnC_Cy

Cuts at:      0     986    1071 
   Size:        986      85
 
HaeII r_GCGC'y

Cuts at:      0     102    1071 
   Size:        102     969
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      12      44     166     198     578     610    1071 
   Size:         12      32     122      32     380      32     461

  Fragments arranged by size: 

                 461     380     122      32      32      32      12
 
HincII GTy'rAC

Cuts at:      0     612    1071 
   Size:        612     459
 
MspA1I CmG'CkG

Cuts at:      0     428     435     896    1071 
   Size:        428       7     461     175

  Fragments arranged by size: 

                 461     428     175       7
 
NspI r_CATG'y

Cuts at:      0     647    1071 
   Size:        647     424
 
PasI CC'CwG_GG

Cuts at:      0     949    1071 
   Size:        949     122
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      75     635    1041    1071 
   Size:         75     560     406      30

  Fragments arranged by size: 

                 560     406      75      30
 
PfoI T'CCnGG_A

Cuts at:      0    1034    1071 
   Size:       1034      37
 
PpuMI rG'GwC_Cy

Cuts at:      0     986    1071 
   Size:        986      85
 
PstI C_TGCA'G

Cuts at:      0     400     830     886    1071 
   Size:        400     430      56     185

  Fragments arranged by size: 

                 430     400     185      56
 
PvuII CAG'CTG

Cuts at:      0     435    1071 
   Size:        435     636
 
SfcI C'TryA_G

Cuts at:      0     396     826     882    1071 
   Size:        396     430      56     189

  Fragments arranged by size: 

                 430     396     189      56
 
SmaI CCC'GGG

Cuts at:      0     119     156     679    1071 
   Size:        119      37     523     392

  Fragments arranged by size: 

                 523     392     119      37
 
SmlI C'TyrA_G

Cuts at:      0     603    1071 
   Size:        603     468
 
SphI G_CATG'C

Cuts at:      0     647    1071 
   Size:        647     424
 
SrfI GCCC'GGGC

Cuts at:      0     119    1071 
   Size:        119     952
 
StuI AGG'CCT

Cuts at:      0     548    1027    1071 
   Size:        548     479      44

  Fragments arranged by size: 

                 548     479      44
 
StyI C'CwwG_G

Cuts at:      0     582    1071 
   Size:        582     489
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     993    1025    1071 
   Size:        993      32      46

  Fragments arranged by size: 

                 993      46      32
 
XmaI C'CCGG_G

Cuts at:      0     117     154     677    1071 
   Size:        117      37     523     394

  Fragments arranged by size: 

                 523     394     117      37
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AlfI    AlwNI    ApaLI     ApoI     AvaI
    AvrII     BanI     BbsI     BcgI     BglI    BglII Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI   BssHII    BssSI   BstAPI   BstEII
    BstYI     BtsI    CspCI   DraIII     DrdI     EaeI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII   MspA1I     NspI     PasI    PflMI
     PfoI    PpuMI     PstI    PvuII     SfcI     SmaI     SmlI     SphI
     SrfI     StuI     StyI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI     AscI     AseI    AsiSI     BaeI    BamHI
    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmtI     BsaI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BstBI
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DraI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SspI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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