Restriction map for pF1KB7337
 (Linear) (Six-base) MAPSORT of: KIBB7337.seq  Check: 8662  from: 1  to: 3129



KIBB7337.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      28    3129 
   Size:         28    3101
 
AccI GT'mk_AC

Cuts at:      0    3103    3129 
   Size:       3103      26
 
Acc65I G'GTAC_C

Cuts at:      0      34    1004    3129 
   Size:         34     970    2125

  Fragments arranged by size: 

                2125     970      34
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1324    2317    2349    2875    3129 
   Size:       1324     993      32     526     254

  Fragments arranged by size: 

                1324     993     526     254      32
 
AflII C'TTAA_G

Cuts at:      0    1111    3129 
   Size:       1111    2018
 
AflIII A'CryG_T

Cuts at:      0    1713    2514    3129 
   Size:       1713     801     615

  Fragments arranged by size: 

                1713     801     615
 
AhdI GACnn_n'nnGTC

Cuts at:      0     132    3129 
   Size:        132    2997
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2664    3129 
   Size:       2664     465
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1278    1310    3129 
   Size:       1278      32    1819

  Fragments arranged by size: 

                1819    1278      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120    2277    2784    3129 
   Size:        120    2157     507     345

  Fragments arranged by size: 

                2157     507     345     120
 
ApaI G_GGCC'C

Cuts at:      0    1446    3129 
   Size:       1446    1683
 
ApoI r'AATT_y

Cuts at:      0      91    1663    2102    2787    3129 
   Size:         91    1572     439     685     342

  Fragments arranged by size: 

                1572     685     439     342      91
 
AvaI C'yCGr_G

Cuts at:      0    3124    3129 
   Size:       3124       5
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      18      51    3129 
   Size:         18      33    3078

  Fragments arranged by size: 

                3078      33      18
 
BamHI G'GATC_C

Cuts at:      0     657    3129 
   Size:        657    2472
 
BanI G'GyrC_C

Cuts at:      0      34    1004    3129 
   Size:         34     970    2125

  Fragments arranged by size: 

                2125     970      34
 
BanII G_rGCy'C

Cuts at:      0     678    1016    1446    2621    3129 
   Size:        678     338     430    1175     508

  Fragments arranged by size: 

                1175     678     508     430     338
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     727    1508    1949    3129 
   Size:        727     781     441    1180

  Fragments arranged by size: 

                1180     781     727     441
 
BbvCI CC'TCA_GC

Cuts at:      0    2842    3129 
   Size:       2842     287
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     859     893    3129 
   Size:        859      34    2236

  Fragments arranged by size: 

                2236     859      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     172    3054    3129 
   Size:        172    2882      75

  Fragments arranged by size: 

                2882     172      75
 
BclI T'GATC_A

Cuts at:      0    1079    3129 
   Size:       1079    2050
 
BglII A'GATC_T

Cuts at:      0     360    1107    3129 
   Size:        360     747    2022

  Fragments arranged by size: 

                2022     747     360
 
Bme1580I G_kGCm'C

Cuts at:      0     152    1272    1446    3129 
   Size:        152    1120     174    1683

  Fragments arranged by size: 

                1683    1120     174     152
 
BmgBI CAC'GTC

Cuts at:      0    1716    2282    3129 
   Size:       1716     566     847

  Fragments arranged by size: 

                1716     847     566
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     762    1012    3129 
   Size:        762     250    2117

  Fragments arranged by size: 

                2117     762     250
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     981    3129 
   Size:        981    2148
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     586    1318    1730    2128    2470    2635    3129 
   Size:        106     480     732     412     398     342     165     494

  Fragments arranged by size: 

                 732     494     480     412     398     342     165     106
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2059    2842    3129 
   Size:       2059     783     287

  Fragments arranged by size: 

                2059     783     287
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198    1188    2419    3129 
   Size:        198     990    1231     710

  Fragments arranged by size: 

                1231     990     710     198
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2239    3129 
   Size:       2239     890
 
BsaAI yAC'GTr

Cuts at:      0     603    2517    3129 
   Size:        603    1914     612

  Fragments arranged by size: 

                1914     612     603
 
BsaBI GATnn'nnATC

Cuts at:      0      77    1084    3129 
   Size:         77    1007    2045

  Fragments arranged by size: 

                2045    1007      77
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1375    1405    2608    2638    3129 
   Size:       1375      30    1203      30     491

  Fragments arranged by size: 

                1375    1203     491      30      30
 
BseYI C'CCAG_C

Cuts at:      0     113    2379    3129 
   Size:        113    2266     750

  Fragments arranged by size: 

                2266     750     113
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     733    3129 
   Size:        346     387    2396

  Fragments arranged by size: 

                2396     387     346
 
BsiHKAI G_wGCw'C

Cuts at:      0    1016    2621    3129 
   Size:       1016    1605     508

  Fragments arranged by size: 

                1605    1016     508
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2717    3129 
   Size:       2717     412
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     678    1016    1272    1446    2621    3129 
   Size:        152     526     338     256     174    1175     508

  Fragments arranged by size: 

                1175     526     508     338     256     174     152
 
BspHI T'CATG_A

Cuts at:      0    1184    3129 
   Size:       1184    1945
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      28    2783    3129 
   Size:         28    2755     346

  Fragments arranged by size: 

                2755     346      28
 
BsrDI GCAATG_nn'

Cuts at:      0     740    2941    3057    3129 
   Size:        740    2201     116      72

  Fragments arranged by size: 

                2201     740     116      72
 
BsrGI T'GTAC_A

Cuts at:      0     462     994    1140    3129 
   Size:        462     532     146    1989

  Fragments arranged by size: 

                1989     532     462     146
 
BssSI C'ACGA_G

Cuts at:      0    1927    3129 
   Size:       1927    1202
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     744    3129 
   Size:        744    2385
 
BstEII G'GTnAC_C

Cuts at:      0    2770    3129 
   Size:       2770     359
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1436    1836    2557    3129 
   Size:       1436     400     721     572

  Fragments arranged by size: 

                1436     721     572     400
 
BstYI r'GATC_y

Cuts at:      0     345     360     414     483     657    1107    3129 
   Size:        345      15      54      69     174     450    2022

  Fragments arranged by size: 

                2022     450     345     174      69      54      15
 
BtgI C'CryG_G

Cuts at:      0    1274    3129 
   Size:       1274    1855
 
BtsI GCAGTG_nn'

Cuts at:      0    1272    1917    3129 
   Size:       1272     645    1212

  Fragments arranged by size: 

                1272    1212     645
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1563    1598    3129 
   Size:       1563      35    1531

  Fragments arranged by size: 

                1563    1531      35
 
DraI TTT'AAA

Cuts at:      0    1775    3129 
   Size:       1775    1354
 
DraIII CAC_nnn'GTG

Cuts at:      0     292    2938    3129 
   Size:        292    2646     191

  Fragments arranged by size: 

                2646     292     191
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2731    3129 
   Size:       2731     398
 
EaeI y'GGCC_r

Cuts at:      0    1426    1457    3129 
   Size:       1426      31    1672

  Fragments arranged by size: 

                1672    1426      31
 
EarI CTCTTCn'nnn_

Cuts at:      0     884    1856    3004    3129 
   Size:        884     972    1148     125

  Fragments arranged by size: 

                1148     972     884     125
 
EcoICRI GAG'CTC

Cuts at:      0    1014    2619    3129 
   Size:       1014    1605     510

  Fragments arranged by size: 

                1605    1014     510
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     586    1318    1324    1730    2128    2317    2349 
   Size:        106     480     732       6     406     398     189      32

Cuts at:   2349    2470    2635    2875    3129 
   Size:        121     165     240     254

  Fragments arranged by size: 

                 732     480     406     398     254     240     189     165
                 121     106      32       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2746    3129 
   Size:       2746     383
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1442    1443    1518    2316    2484    3129 
   Size:       1442       1      75     798     168     645

  Fragments arranged by size: 

                1442     798     645     168      75       1
 
EcoRI G'AATT_C

Cuts at:      0      91    1663    3129 
   Size:         91    1572    1466

  Fragments arranged by size: 

                1572    1466      91
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     120     744    2002    2619    3129 
   Size:        120     624    1258     617     510

  Fragments arranged by size: 

                1258     624     617     510     120
 
HaeII r_GCGC'y

Cuts at:      0     651    3129 
   Size:        651    2478
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150     261     293     883     915     970    1002 
   Size:        118      32     111      32     590      32      55      32

Cuts at:   1002    1321    1353    1950    1982    2252    2284    2603    2635 
   Size:        319      32     597      32     270      32     319      32

Cuts at:   2635    3129 
   Size:        494

  Fragments arranged by size: 

                 597     590     494     319     319     270     118     111
                  55      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1983    3104    3129 
   Size:       1983    1121      25

  Fragments arranged by size: 

                1983    1121      25
 
HindIII A'AGCT_T

Cuts at:      0      97    2015    2408    3129 
   Size:         97    1918     393     721

  Fragments arranged by size: 

                1918     721     393      97
 
KpnI G_GTAC'C

Cuts at:      0      38    1008    3129 
   Size:         38     970    2121

  Fragments arranged by size: 

                2121     970      38
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     373    1264    1406    2535    3129 
   Size:        373     891     142    1129     594

  Fragments arranged by size: 

                1129     891     594     373     142
 
MscI TGG'CCA

Cuts at:      0    1428    1459    3129 
   Size:       1428      31    1670

  Fragments arranged by size: 

                1670    1428      31
 
MslI CAynn'nnrTG

Cuts at:      0    1196    3129 
   Size:       1196    1933
 
MspA1I CmG'CkG

Cuts at:      0      84     120     806    1424    2477    2634    3129 
   Size:         84      36     686     618    1053     157     495

  Fragments arranged by size: 

                1053     686     618     495     157      84      36
 
NcoI C'CATG_G

Cuts at:      0    1274    3129 
   Size:       1274    1855
 
NspI r_CATG'y

Cuts at:      0    3110    3129 
   Size:       3110      19
 
PasI CC'CwG_GG

Cuts at:      0     144     167     402     471    1829    3129 
   Size:        144      23     235      69    1358    1300

  Fragments arranged by size: 

                1358    1300     235     144      69      23
 
PfoI T'CCnGG_A

Cuts at:      0    1616    2128    3129 
   Size:       1616     512    1001

  Fragments arranged by size: 

                1616    1001     512
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2353    2385    3129 
   Size:       2353      32     744

  Fragments arranged by size: 

                2353     744      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2316    2484    3129 
   Size:       2316     168     645

  Fragments arranged by size: 

                2316     645     168
 
PshAI GACnn'nnGTC

Cuts at:      0     691    3129 
   Size:        691    2438
 
PspOMI G'GGCC_C

Cuts at:      0    1442    3129 
   Size:       1442    1687
 
PstI C_TGCA'G

Cuts at:      0     647    1229    1267    1453    2315    2678    2780    3119 
   Size:        647     582      38     186     862     363     102     339

Cuts at:   3119    3129 
   Size:         10

  Fragments arranged by size: 

                 862     647     582     363     339     186     102      38
                  10
 
PvuII CAG'CTG

Cuts at:      0      84     120     806    1424    2477    2634    3129 
   Size:         84      36     686     618    1053     157     495

  Fragments arranged by size: 

                1053     686     618     495     157      84      36
 
SacI G_AGCT'C

Cuts at:      0    1016    2621    3129 
   Size:       1016    1605     508

  Fragments arranged by size: 

                1605    1016     508
 
SalI G'TCGA_C

Cuts at:      0    3102    3129 
   Size:       3102      27
 
SexAI A'CCwGG_T

Cuts at:      0    1093    3129 
   Size:       1093    2036
 
SfcI C'TryA_G

Cuts at:      0     643    1225    1263    1449    2311    2674    2776    3115 
   Size:        643     582      38     186     862     363     102     339

Cuts at:   3115    3129 
   Size:         14

  Fragments arranged by size: 

                 862     643     582     363     339     186     102      38
                  14
 
SmlI C'TyrA_G

Cuts at:      0     177    1111    1203    2434    3129 
   Size:        177     934      92    1231     695

  Fragments arranged by size: 

                1231     934     695     177      92
 
SspI AAT'ATT

Cuts at:      0    1560    1992    3129 
   Size:       1560     432    1137

  Fragments arranged by size: 

                1560    1137     432
 
StuI AGG'CCT

Cuts at:      0    1117    2752    3129 
   Size:       1117    1635     377

  Fragments arranged by size: 

                1635    1117     377
 
StyI C'CwwG_G

Cuts at:      0      42     728    1274    2753    3129 
   Size:         42     686     546    1479     376

  Fragments arranged by size: 

                1479     686     546     376      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1618    3129 
   Size:       1618    1511
 
TatI w'GTAC_w

Cuts at:      0     462     994    1140    3129 
   Size:        462     532     146    1989

  Fragments arranged by size: 

                1989     532     462     146
 
XbaI T'CTAG_A

Cuts at:      0     363    3129 
   Size:        363    2766
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     998    3129 
   Size:        998    2131
 
XmnI GAAnn'nnTTC

Cuts at:      0    1058    3129 
   Size:       1058    2071
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII   AflIII     AhdI     AlfI
     AloI    AlwNI     ApaI     ApoI     AvaI     BaeI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI    BciVI     BclI    BglII Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaXI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BspMI    BsrDI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI
     BtgI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI
     NspI     PasI     PfoI     PpiI    PpuMI    PshAI   PspOMI     PstI
    PvuII     SacI     SalI    SexAI     SfcI     SmlI     SspI     StuI
     StyI    TaqII     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI     AgeI     AleI    ApaLI     AscI     AseI
    AsiSI    AvrII     BbeI    BfrBI     BglI     BlpI     BmtI    BsaHI
    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BsrBI    BsrFI
   BssHII    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI     EagI     EciI
    EcoRV     FseI     FspI    FspAI     HpaI     KasI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PmeI     PmlI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI     TstI  Tth111I     XhoI     XmaI     ZraI
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