Restriction map for pF1KB6454
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HindIII     KasI        
KpnI        KspI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AciI          199  CCGC                 CCGC(-3/-1)
AclI          433  AACGTT               AA^CGTT
AcyI          325  GGCGTC               G[GA]^CG[CT]C
AlwI          258  GATCC                GGATC(4/5)
AlwNI        1019  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          544  ATTAAT               AT^TAAT
AsnI          544  ATTAAT               AT^TAAT
AspI          457  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII        1032  GGACC                G^G[AT]CC
BanII         263  GGGCTC               G[GA]GC[CT]^C
BbsI          618  GAAGAC               GAAGAC(2/6)
BcgI           40  GCATCCCTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          515  CTAG                 C^TAG
BlpI          755  GCTTAGC              GC^T[ACGT]AGC
BmyI          263  GGGCTC               G[AGT]GC[ACT]^C
Bpu1102I      755  GCTTAGC              GC^T[ACGT]AGC
BpuAI         618  GAAGAC               GAAGAC(2/6)
BsaHI         325  GGCGTC               G[GA]^CG[CT]C
Bsp1286I      263  GGGCTC               G[AGT]GC[ACT]^C
BspHI         882  TCATGA               T^CATGA
BspMI         576  GCAGGT               ACCTGC(4/8)
CelII         755  GCTTAGC              GC^T[ACGT]AGC
DraII        1031  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoNI         135  CCTGACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1031  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI         427  GAATTC               G^AATTC
HaeII         248  AGCGCC               [GA]GCGC^[CT]
HgaI          326  GCGTC                GACGC(5/10)
Hin1I         325  GGCGTC               G[GA]^CG[CT]C
HpaI          436  GTTAAC               GTT^AAC
MaeI          515  CTAG                 C^TAG
MaeII         434  ACGT                 A^CGT
PflFI         457  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         447  CCAAGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1031  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         544  ATTAAT               AT^TAAT
Psp1406I      433  AACGTT               AA^CGTT
RcaI          882  TCATGA               T^CATGA
SmlI          788  CTCAAG               C^T[CT][GA]AG
SspI         1006  AATATT               AAT^ATT
Tth111I       457  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        447  CCAAGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI          427  GAATTC               [GA]^AATT[CT]
              626  AAATTC              
AfaI          362  GTAC                 GT^AC
              557  GTAC                
AluI          357  AGCT                 AG^CT
              390  AGCT                
ApoI          427  GAATTC               [GA]^AATT[CT]
              626  AAATTC              
BcnI          261  CCGGG                CC^[GC]GG
              596  CCCGG               
BseRI         293  CTCCTC               GAGGAG(10/8)
              154  GAGGAG              
DpnII         258  GATC                 ^GATC
              718  GATC                
EarI          118  CTCTTC               CTCTTC(1/4)
             1037  CTCTTC              
EcoT14I       762  CCTTGG               C^C[AT][AT]GG
              906  CCAAGG              
HaeIII        371  GGCC                 GG^CC
              804  GGCC                
HapII         261  CCGG                 C^CGG
              597  CCGG                
HincII        328  GTCAAC               GT[CT]^[GA]AC
              436  GTTAAC              
HindII        328  GTCAAC               GT[CT]^[GA]AC
              436  GTTAAC              
HpaII         261  CCGG                 C^CGG
              597  CCGG                
HphI          255  GGTGA                GGTGA(8/7)
              868  GGTGA               
Ksp632I       118  CTCTTC               CTCTTC(1/4)
             1037  CTCTTC              
MaeIII        499  GTCAC                ^GT[ACGT]AC
              633  GTCAC               
MboI          258  GATC                 ^GATC
              718  GATC                
MseI          437  TTAA                 T^TAA
              545  TTAA                
MspI          261  CCGG                 C^CGG
              597  CCGG                
MwoI          304  GCCTATGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              805  GCCCTGTGTGC         
NciI          261  CCGGG                CC^[GC]GG
              596  CCCGG               
NdeII         258  GATC                 ^GATC
              718  GATC                
PleI          278  GAGTC                GAGTC(4/5)
              784  GAGTC               
PstI          470  CTGCAG               CTGCA^G
              835  CTGCAG              
RsaI          362  GTAC                 GT^AC
              557  GTAC                
Sau3AI        258  GATC                 ^GATC
              718  GATC                
StyI          762  CCTTGG               C^C[AT][AT]GG
              906  CCAAGG              
TaqI          720  TCGA                 T^CGA
              969  TCGA                
TfiI          691  GAATC                G^A[AT]TC
              982  GATTC               
Tru9I         437  TTAA                 T^TAA
              545  TTAA                
Tsp45I        499  GTCAC                ^GT[GC]AC
              633  GTCAC               
TthHB8I       720  TCGA                 T^CGA
              969  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

BbvI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       BsrI        BssKI       BstF5I      
BstNI       CfoI        Cfr13I      DdeI        EcoRII      
Fnu4HI      FokI        HhaI        HinP1I      HinfI       
ItaI        MboII       MnlI        MvaI        NlaIII      
NlaIV       Sau96I      ScrFI       SfaNI       SfcI        
TseI        Tsp509I     TspRI       
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