Restriction map for pF1KB7828
 (Linear) (Six-base) MAPSORT of: KIBB7828.seq  Check: 2540  from: 1  to: 1815



KIBB7828.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     912    1815 
   Size:        912     903
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     744    1148    1815 
   Size:         99     645     404     667

  Fragments arranged by size: 

                 667     645     404      99
 
AleI CACnn'nnGTG

Cuts at:      0    1246    1815 
   Size:       1246     569
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     910    1264    1815 
   Size:        910     354     551

  Fragments arranged by size: 

                 910     551     354
 
ApaI G_GGCC'C

Cuts at:      0     947    1815 
   Size:        947     868
 
ApoI r'AATT_y

Cuts at:      0     244     813     850    1012    1815 
   Size:        244     569      37     162     803

  Fragments arranged by size: 

                 803     569     244     162      37
 
AseI AT'TA_AT

Cuts at:      0    1489    1815 
   Size:       1489     326
 
BanII G_rGCy'C

Cuts at:      0     460     878     947    1815 
   Size:        460     418      69     868

  Fragments arranged by size: 

                 868     460     418      69
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     456     788    1815 
   Size:        456     332    1027

  Fragments arranged by size: 

                1027     456     332
 
BglII A'GATC_T

Cuts at:      0      84    1815 
   Size:         84    1731
 
BlpI GC'TnA_GC

Cuts at:      0     872    1815 
   Size:        872     943
 
Bme1580I G_kGCm'C

Cuts at:      0     947    1152    1815 
   Size:        947     205     663

  Fragments arranged by size: 

                 947     663     205
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     454     872    1049    1815 
   Size:        454     418     177     766

  Fragments arranged by size: 

                 766     454     418     177
 
Bpu10I CC'TnA_GC

Cuts at:      0     509    1815 
   Size:        509    1306
 
BsaAI yAC'GTr

Cuts at:      0    1248    1504    1705    1815 
   Size:       1248     256     201     110

  Fragments arranged by size: 

                1248     256     201     110
 
BsaBI GATnn'nnATC

Cuts at:      0      83    1586    1815 
   Size:         83    1503     229

  Fragments arranged by size: 

                1503     229      83
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     580    1815 
   Size:        580    1235
 
BseYI C'CCAG_C

Cuts at:      0      38     266    1815 
   Size:         38     228    1549

  Fragments arranged by size: 

                1549     228      38
 
BsiHKAI G_wGCw'C

Cuts at:      0    1243    1815 
   Size:       1243     572
 
Bsp1286I G_dGCh'C

Cuts at:      0     460     878     947    1152    1243    1815 
   Size:        460     418      69     205      91     572

  Fragments arranged by size: 

                 572     460     418     205      91      69
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     912    1815 
   Size:        912     903
 
BsrDI GCAATG_nn'

Cuts at:      0     467    1773    1815 
   Size:        467    1306      42

  Fragments arranged by size: 

                1306     467      42
 
BstYI r'GATC_y

Cuts at:      0      84     292    1815 
   Size:         84     208    1523

  Fragments arranged by size: 

                1523     208      84
 
BtgI C'CryG_G

Cuts at:      0     632    1815 
   Size:        632    1183
 
DraIII CAC_nnn'GTG

Cuts at:      0    1080    1815 
   Size:       1080     735
 
EarI CTCTTCn'nnn_

Cuts at:      0     227    1130    1815 
   Size:        227     903     685

  Fragments arranged by size: 

                 903     685     227
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     744    1148    1815 
   Size:         99     645     404     667

  Fragments arranged by size: 

                 667     645     404      99
 
EcoRI G'AATT_C

Cuts at:      0    1012    1815 
   Size:       1012     803
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1498    1815 
   Size:       1498     317
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     559     591    1012    1044    1815 
   Size:        559      32     421      32     771

  Fragments arranged by size: 

                 771     559     421      32      32
 
HincII GTy'rAC

Cuts at:      0     669     797     831    1437    1815 
   Size:        669     128      34     606     378

  Fragments arranged by size: 

                 669     606     378     128      34
 
HindIII A'AGCT_T

Cuts at:      0     977    1815 
   Size:        977     838
 
MslI CAynn'nnrTG

Cuts at:      0    1246    1250    1815 
   Size:       1246       4     565

  Fragments arranged by size: 

                1246     565       4
 
NcoI C'CATG_G

Cuts at:      0     632    1815 
   Size:        632    1183
 
NspI r_CATG'y

Cuts at:      0     360     897    1701    1815 
   Size:        360     537     804     114

  Fragments arranged by size: 

                 804     537     360     114
 
PmlI CAC'GTG

Cuts at:      0    1248    1815 
   Size:       1248     567
 
PshAI GACnn'nnGTC

Cuts at:      0      33     116    1815 
   Size:         33      83    1699

  Fragments arranged by size: 

                1699      83      33
 
PspOMI G'GGCC_C

Cuts at:      0     943    1815 
   Size:        943     872
 
PstI C_TGCA'G

Cuts at:      0    1751    1815 
   Size:       1751      64
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1130    1815 
   Size:       1130     685
 
SfcI C'TryA_G

Cuts at:      0     723    1406    1747    1815 
   Size:        723     683     341      68

  Fragments arranged by size: 

                 723     683     341      68
 
SnaBI TAC'GTA

Cuts at:      0    1504    1815 
   Size:       1504     311
 
SphI G_CATG'C

Cuts at:      0     360     897    1701    1815 
   Size:        360     537     804     114

  Fragments arranged by size: 

                 804     537     360     114
 
SspI AAT'ATT

Cuts at:      0     603    1815 
   Size:        603    1212
 
StyI C'CwwG_G

Cuts at:      0     632    1815 
   Size:        632    1183
 
TatI w'GTAC_w

Cuts at:      0     341    1188    1815 
   Size:        341     847     627

  Fragments arranged by size: 

                 847     627     341
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     ApaI     ApoI     AseI    BanII
     BbsI    BglII     BlpI Bme1580I     BmrI   Bpu10I    BsaAI    BsaBI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrDI    BstYI     BtgI
   DraIII     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII  HindIII
     MslI     NcoI     NspI     PmlI    PshAI   PspOMI     PstI     SapI
     SfcI    SnaBI     SphI     SspI     StyI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI     SpeI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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