Restriction map for pF1KB6155
 (Linear) (Six-base) MAPSORT of: KIBB6155.seq  Check: 3644  from: 1  to: 1791



KIBB6155.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AatII G_ACGT'C

Cuts at:      0    1676    1791 
   Size:       1676     115
 
AleI CACnn'nnGTG

Cuts at:      0    1354    1791 
   Size:       1354     437
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1106    1791 
   Size:       1106     685
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     232     264    1791 
   Size:        232      32    1527

  Fragments arranged by size: 

                1527     232      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     117     795    1503    1791 
   Size:         18      99     678     708     288

  Fragments arranged by size: 

                 708     678     288      99      18
 
ApaI G_GGCC'C

Cuts at:      0     413    1791 
   Size:        413    1378
 
AvaI C'yCGr_G

Cuts at:      0     289    1544    1791 
   Size:        289    1255     247

  Fragments arranged by size: 

                1255     289     247
 
BanI G'GyrC_C

Cuts at:      0      86     549     887    1299    1791 
   Size:         86     463     338     412     492

  Fragments arranged by size: 

                 492     463     412     338      86
 
BanII G_rGCy'C

Cuts at:      0      10     155     413     565     605     712    1025    1238 
   Size:         10     145     258     152      40     107     313     213

Cuts at:   1238    1791 
   Size:        553

  Fragments arranged by size: 

                 553     313     258     213     152     145     107      40
                  10
 
BbeI G_GCGC'C

Cuts at:      0      90    1791 
   Size:         90    1701
 
BclI T'GATC_A

Cuts at:      0    1358    1791 
   Size:       1358     433
 
BglI GCCn_nnn'nGGC

Cuts at:      0      41    1791 
   Size:         41    1750
 
Bme1580I G_kGCm'C

Cuts at:      0     413    1304    1791 
   Size:        413     891     487

  Fragments arranged by size: 

                 891     487     413
 
BmgBI CAC'GTC

Cuts at:      0    1784    1791 
   Size:       1784       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      79    1388    1491    1791 
   Size:         79    1309     103     300

  Fragments arranged by size: 

                1309     300     103      79
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240     994    1012    1081    1525    1738    1791 
   Size:        240     754      18      69     444     213      53

  Fragments arranged by size: 

                 754     444     240     213      69      53      18
 
Bpu10I CC'TnA_GC

Cuts at:      0      50    1791 
   Size:         50    1741
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1213    1791 
   Size:       1213     578
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     185    1791 
   Size:        185    1606
 
BsaBI GATnn'nnATC

Cuts at:      0    1693    1791 
   Size:       1693      98
 
BsaHI Gr'CG_yC

Cuts at:      0      87    1673    1791 
   Size:         87    1586     118

  Fragments arranged by size: 

                1586     118      87
 
BsaWI w'CCGG_w

Cuts at:      0     233    1791 
   Size:        233    1558
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     232     262     592     622    1477    1507    1579    1609 
   Size:        232      30     330      30     855      30      72      30

Cuts at:   1609    1791 
   Size:        182

  Fragments arranged by size: 

                 855     330     232     182      72      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     288     840    1033    1152    1245    1720    1791 
   Size:        288     552     193     119      93     475      71

  Fragments arranged by size: 

                 552     475     288     193     119      93      71
 
BseYI C'CCAG_C

Cuts at:      0     405     553     795     869    1590    1791 
   Size:        405     148     242      74     721     201

  Fragments arranged by size: 

                 721     405     242     201     148      74
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     319    1231    1591    1791 
   Size:        130     189     912     360     200

  Fragments arranged by size: 

                 912     360     200     189     130
 
BsiEI CG_ry'CG

Cuts at:      0    1141    1791 
   Size:       1141     650
 
BsiHKAI G_wGCw'C

Cuts at:      0      26    1025    1070    1791 
   Size:         26     999      45     721

  Fragments arranged by size: 

                 999     721      45      26
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1680    1774    1791 
   Size:       1680      94      17

  Fragments arranged by size: 

                1680      94      17
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      26     155     413     565     605     712    1025 
   Size:         10      16     129     258     152      40     107     313

Cuts at:   1025    1070    1238    1304    1791 
   Size:         45     168      66     487

  Fragments arranged by size: 

                 487     313     258     168     152     129     107      66
                  45      40      16      10
 
BspEI T'CCGG_A

Cuts at:      0     233    1791 
   Size:        233    1558
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     251    1791 
   Size:        251    1540
 
BsrBI CCG'CTC

Cuts at:      0     932     978    1791 
   Size:        932      46     813

  Fragments arranged by size: 

                 932     813      46
 
BsrFI r'CCGG_y

Cuts at:      0      89     658    1040    1577    1791 
   Size:         89     569     382     537     214

  Fragments arranged by size: 

                 569     537     382     214      89
 
BssSI C'ACGA_G

Cuts at:      0     858     892    1198    1791 
   Size:        858      34     306     593

  Fragments arranged by size: 

                 858     593     306      34
 
BstBI TT'CG_AA

Cuts at:      0     941    1791 
   Size:        941     850
 
BstYI r'GATC_y

Cuts at:      0     494    1193    1791 
   Size:        494     699     598

  Fragments arranged by size: 

                 699     598     494
 
BtgI C'CryG_G

Cuts at:      0     832    1791 
   Size:        832     959
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     404     897    1140    1791 
   Size:        404     493     243     651

  Fragments arranged by size: 

                 651     493     404     243
 
BtsI GCAGTG_nn'

Cuts at:      0     251    1791 
   Size:        251    1540
 
DraIII CAC_nnn'GTG

Cuts at:      0     894    1791 
   Size:        894     897
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     277    1791 
   Size:        277    1514
 
EaeI y'GGCC_r

Cuts at:      0     323    1138    1791 
   Size:        323     815     653

  Fragments arranged by size: 

                 815     653     323
 
EagI C'GGCC_G

Cuts at:      0    1138    1791 
   Size:       1138     653
 
EarI CTCTTCn'nnn_

Cuts at:      0      31     823    1791 
   Size:         31     792     968

  Fragments arranged by size: 

                 968     792      31
 
EcoICRI GAG'CTC

Cuts at:      0    1023    1791 
   Size:       1023     768
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240     994    1012    1081    1525    1738    1791 
   Size:        240     754      18      69     444     213      53

  Fragments arranged by size: 

                 754     444     240     213      69      53      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     544    1283    1791 
   Size:        544     739     508

  Fragments arranged by size: 

                 739     544     508
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     409    1145    1245    1288    1369    1791 
   Size:        114     295     736     100      43      81     422

  Fragments arranged by size: 

                 736     422     295     114     100      81      43
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     333    1791 
   Size:        333    1458
 
HaeII r_GCGC'y

Cuts at:      0      90     368    1770    1791 
   Size:         90     278    1402      21

  Fragments arranged by size: 

                1402     278      90      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      57      89     592     624     835     867    1388    1420 
   Size:         57      32     503      32     211      32     521      32

Cuts at:   1420    1579    1611    1791 
   Size:        159      32     180

  Fragments arranged by size: 

                 521     503     211     180     159      57      32      32
                  32      32      32
 
KasI G'GCGC_C

Cuts at:      0      86    1791 
   Size:         86    1705
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     668     782    1097    1791 
   Size:         41     627     114     315     694

  Fragments arranged by size: 

                 694     627     315     114      41
 
MscI TGG'CCA

Cuts at:      0     325    1791 
   Size:        325    1466
 
MslI CAynn'nnrTG

Cuts at:      0    1354    1791 
   Size:       1354     437
 
MspA1I CmG'CkG

Cuts at:      0     576     780     834    1090    1460    1669    1791 
   Size:        576     204      54     256     370     209     122

  Fragments arranged by size: 

                 576     370     256     209     204     122      54
 
NaeI GCC'GGC

Cuts at:      0      91    1791 
   Size:         91    1700
 
NarI GG'CG_CC

Cuts at:      0      87    1791 
   Size:         87    1704
 
NgoMIV G'CCGG_C

Cuts at:      0      89    1791 
   Size:         89    1702
 
NruI TCG'CGA

Cuts at:      0      77    1791 
   Size:         77    1714
 
PasI CC'CwG_GG

Cuts at:      0    1279    1422    1791 
   Size:       1279     143     369

  Fragments arranged by size: 

                1279     369     143
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     795     906     972    1791 
   Size:        795     111      66     819

  Fragments arranged by size: 

                 819     795     111      66
 
PfoI T'CCnGG_A

Cuts at:      0     501     674    1058    1406    1583    1700    1791 
   Size:        501     173     384     348     177     117      91

  Fragments arranged by size: 

                 501     384     348     177     173     117      91
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     232     264    1791 
   Size:        232      32    1527

  Fragments arranged by size: 

                1527     232      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1288    1791 
   Size:       1288     503
 
PspOMI G'GGCC_C

Cuts at:      0     409    1791 
   Size:        409    1382
 
PstI C_TGCA'G

Cuts at:      0     353     830    1193    1723    1791 
   Size:        353     477     363     530      68

  Fragments arranged by size: 

                 530     477     363     353      68
 
PvuII CAG'CTG

Cuts at:      0    1090    1791 
   Size:       1090     701
 
SacI G_AGCT'C

Cuts at:      0    1025    1791 
   Size:       1025     766
 
SacII CC_GC'GG

Cuts at:      0     835    1791 
   Size:        835     956
 
SapI GCTCTTCn'nnn_

Cuts at:      0      31     823    1791 
   Size:         31     792     968

  Fragments arranged by size: 

                 968     792      31
 
SfcI C'TryA_G

Cuts at:      0     179     349     826    1189    1205    1719    1791 
   Size:        179     170     477     363      16     514      72

  Fragments arranged by size: 

                 514     477     363     179     170      72      16
 
SfoI GGC'GCC

Cuts at:      0      88    1791 
   Size:         88    1703
 
SgrAI Cr'CCGG_yG

Cuts at:      0      89    1791 
   Size:         89    1702
 
SmaI CCC'GGG

Cuts at:      0    1546    1791 
   Size:       1546     245
 
SmlI C'TyrA_G

Cuts at:      0    1228    1791 
   Size:       1228     563
 
StyI C'CwwG_G

Cuts at:      0     186    1008    1791 
   Size:        186     822     783

  Fragments arranged by size: 

                 822     783     186
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     567     648    1299    1791 
   Size:        567      81     651     492

  Fragments arranged by size: 

                 651     567     492      81
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     880     912    1477    1509    1754    1786    1791 
   Size:        880      32     565      32     245      32       5

  Fragments arranged by size: 

                 880     565     245      32      32      32       5
 
Tth111I GACn'n_nGTC

Cuts at:      0      71    1791 
   Size:         71    1720
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1429    1708    1791 
   Size:       1429     279      83

  Fragments arranged by size: 

                1429     279      83
 
XmaI C'CCGG_G

Cuts at:      0    1544    1791 
   Size:       1544     247
 
XmnI GAAnn'nnTTC

Cuts at:      0     214     622    1791 
   Size:        214     408    1169

  Fragments arranged by size: 

                1169     408     214
 
ZraI GAC'GTC

Cuts at:      0    1674    1791 
   Size:       1674     117
 

 Enzymes that do cut:

    AatII     AleI     AlfI     AloI    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI     BclI     BglI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BssSI    BstBI    BstYI     BtgI    BtgZI     BtsI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NruI     PasI    PflMI     PfoI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII     SapI
     SfcI     SfoI    SgrAI     SmaI     SmlI     StyI    TaqII     TstI
  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII
     BlpI     BmtI     BplI    BsaAI    BsiWI     BsmI    BspHI    BsrDI
    BsrGI   BssHII   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NdeI
     NheI     NotI     NsiI     NspI     PacI     PciI     PmeI     PmlI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     XbaI     XhoI
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