(Linear) (Six-base) MAPSORT of: KIEE0427.seq  Check: 9549  from: 1  to: 534



KIEE0427.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AflIII A'CryG_T

Cuts at:      0     362     534 
   Size:        362     172
 
ApoI r'AATT_y

Cuts at:      0     244     534 
   Size:        244     290
 
AseI AT'TA_AT

Cuts at:      0     157     534 
   Size:        157     377
 
BamHI G'GATC_C

Cuts at:      0      60     534 
   Size:         60     474
 
BanI G'GyrC_C

Cuts at:      0     141     534 
   Size:        141     393
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     530     534 
   Size:        530       4
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     468     534 
   Size:        468      66
 
Bme1580I G_kGCm'C

Cuts at:      0     218     534 
   Size:        218     316
 
BmgBI CAC'GTC

Cuts at:      0     219     534 
   Size:        219     315
 
BsaHI Gr'CG_yC

Cuts at:      0     252     534 
   Size:        252     282
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     304     534 
   Size:        304     230
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     534 
   Size:        218     316
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      31     534 
   Size:         31     503
 
BsrGI T'GTAC_A

Cuts at:      0     365     534 
   Size:        365     169
 
BstYI r'GATC_y

Cuts at:      0      60     324     534 
   Size:         60     264     210

  Fragments arranged by size: 

                 264     210      60
 
BtgI C'CryG_G

Cuts at:      0      10     534 
   Size:         10     524
 
BtsI GCAGTG_nn'

Cuts at:      0     143     534 
   Size:        143     391
 
DraIII CAC_nnn'GTG

Cuts at:      0     148     534 
   Size:        148     386
 
EaeI y'GGCC_r

Cuts at:      0     115     416     534 
   Size:        115     301     118

  Fragments arranged by size: 

                 301     118     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     472     504     534 
   Size:        472      32      30

  Fragments arranged by size: 

                 472      32      30
 
HincII GTy'rAC

Cuts at:      0     360     534 
   Size:        360     174
 
HpaI GTT'AAC

Cuts at:      0     360     534 
   Size:        360     174
 
MscI TGG'CCA

Cuts at:      0     117     534 
   Size:        117     417
 
NspI r_CATG'y

Cuts at:      0     366     534 
   Size:        366     168
 
PciI A'CATG_T

Cuts at:      0     362     534 
   Size:        362     172
 
ScaI AGT'ACT

Cuts at:      0     409     534 
   Size:        409     125
 
SexAI A'CCwGG_T

Cuts at:      0     144     534 
   Size:        144     390
 
SfcI C'TryA_G

Cuts at:      0     381     534 
   Size:        381     153
 
StuI AGG'CCT

Cuts at:      0     519     534 
   Size:        519      15
 
StyI C'CwwG_G

Cuts at:      0     255     534 
   Size:        255     279
 
TatI w'GTAC_w

Cuts at:      0     365     407     534 
   Size:        365      42     127

  Fragments arranged by size: 

                 365     127      42
 

 Enzymes that do cut:

   AflIII     ApoI     AseI    BamHI     BanI     BbsI    BciVI Bme1580I
    BmgBI    BsaHI    BseRI Bsp1286I    BspMI    BsrGI    BstYI     BtgI
     BtsI   DraIII     EaeI    Hin4I   HincII     HpaI     MscI     NspI
     PciI     ScaI    SexAI     SfcI     StuI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI