Restriction map for pF1KE0473
##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaHI       BsaI        BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
KasI        KpnI        KspI        MaeI        MaeIII      
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AfaI          241  GTAC                 GT^AC
AocI           91  CCTGAGG              CC^T[ACGT]AGG
ApaI          248  GGGCCC               GGGCC^C
AvaII         194  GGACC                G^G[AT]CC
BanI          388  GGCACC               G^G[CT][GA]CC
BanII         248  GGGCCC               G[GA]GC[CT]^C
BlpI          457  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      457  GCTGAGC              GC^T[ACGT]AGC
BsaBI         210  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         191  ACCGGA               [AT]^CCGG[AT]
BsgI           68  CTGCAC               GTGCAG(16/14)
BsmAI         340  GAGAC                GTCTC(1/5)
BsmBI         340  GAGACG               CGTCTC(1/5)
BspMI         108  ACCTGC               ACCTGC(4/8)
BsrDI         437  GCAATG               GCAATG(2/0)
Bsu36I         91  CCTGAGG              CC^T[ACGT]AGG
CelII         457  GCTGAGC              GC^T[ACGT]AGC
DpnII         216  GATC                 ^GATC
EaeI          225  CGGCCA               [CT]^GGCC[GA]
Eco81I         91  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       101  CCAAGG               C^C[AT][AT]GG
HapII         192  CCGG                 C^CGG
HpaII         192  CCGG                 C^CGG
HphI          218  TCACC                GGTGA(8/7)
MamI          210  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          216  GATC                 ^GATC
MslI          444  CACGGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        517  CAGCTG               C[AC]G^C[GT]G
MspI          192  CCGG                 C^CGG
NdeII         216  GATC                 ^GATC
PstI          358  CTGCAG               CTGCA^G
PvuII         517  CAGCTG               CAG^CTG
RsaI          241  GTAC                 GT^AC
Sau3AI        216  GATC                 ^GATC
SfcI          358  CTGCAG               C^T[GA][CT]AG
StuI          173  AGGCCT               AGG^CCT
StyI          101  CCAAGG               C^C[AT][AT]GG
Tsp509I       556  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BmyI          248  GGGCCC               G[AGT]GC[ACT]^C
              387  GGGCAC              
BsiYI         228  CCAGTGCGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              372  CCGCAACAAGG         
BslI          228  CCAGTGCGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              372  CCGCAACAAGG         
Bsp1286I      248  GGGCCC               G[AGT]GC[ACT]^C
              387  GGGCAC              
BsrI          202  ACTGG                ACTGG(1/-1)
              228  CCAGT               
BssKI         252  CCTGG                ^CC[ACGT]GG
              483  CCTGG               
BstNI         252  CCTGG                CC^[AT]GG
              483  CCTGG               
DraII         248  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              410  AGGGCCT             
EarI          160  GAAGAG               CTCTTC(1/4)
              310  GAAGAG              
EcoO109I      248  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              410  AGGGCCT             
EcoRII        252  CCTGG                ^CC[AT]GG
              483  CCTGG               
Ksp632I       160  GAAGAG               CTCTTC(1/4)
              310  GAAGAG              
MaeII         343  ACGT                 A^CGT
              470  ACGT                
MvaI          252  CCTGG                CC^[AT]GG
              483  CCTGG               
NlaIII        450  CATG                 CATG^
              582  CATG                
ScrFI         252  CCTGG                CC^[ACGT]GG
              483  CCTGG               
TspRI         144  CACTG                CA[GC]TG[ACGT][ACGT]^
              229  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AciI        AluI        AlwNI       BbvI        BpmI        
BsaJI       BstF5I      Cac8I       Cfr13I      DdeI        
FauI        Fnu4HI      FokI        HaeIII      ItaI        
MboII       MnlI        MwoI        NlaIV       Sau96I      
SfaNI       TseI        
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