Restriction map for pF1KE0473
 (Linear) (Six-base) MAPSORT of: KIEE0473.seq  Check: 6025  from: 1  to: 591



KIEE0473.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     527     591 
   Size:        329     198      64

  Fragments arranged by size: 

                 329     198      64
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     491     554     591 
   Size:        491      63      37

  Fragments arranged by size: 

                 491      63      37
 
AlwNI CAG_nnn'CTG

Cuts at:      0      67      79      91     591 
   Size:         67      12      12     500

  Fragments arranged by size: 

                 500      67      12      12
 
ApaI G_GGCC'C

Cuts at:      0     252     591 
   Size:        252     339
 
BanI G'GyrC_C

Cuts at:      0     388     591 
   Size:        388     203
 
BanII G_rGCy'C

Cuts at:      0     252     591 
   Size:        252     339
 
BlpI GC'TnA_GC

Cuts at:      0     458     591 
   Size:        458     133
 
Bme1580I G_kGCm'C

Cuts at:      0     252     391     591 
   Size:        252     139     200

  Fragments arranged by size: 

                 252     200     139
 
BmgBI CAC'GTC

Cuts at:      0     471     591 
   Size:        471     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32      38      44     224     591 
   Size:         32       6       6     180     367

  Fragments arranged by size: 

                 367     180      32       6       6
 
Bpu10I CC'TnA_GC

Cuts at:      0      17     591 
   Size:         17     574
 
BsaBI GATnn'nnATC

Cuts at:      0     214     591 
   Size:        214     377
 
BsaWI w'CCGG_w

Cuts at:      0     191     591 
   Size:        191     400
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     505     535     591 
   Size:        505      30      56

  Fragments arranged by size: 

                 505      56      30
 
BseYI C'CCAG_C

Cuts at:      0      84     291     526     591 
   Size:         84     207     235      65

  Fragments arranged by size: 

                 235     207      84      65
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     591 
   Size:         53     538
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     334     591 
   Size:        334     257
 
Bsp1286I G_dGCh'C

Cuts at:      0     252     391     591 
   Size:        252     139     200

  Fragments arranged by size: 

                 252     200     139
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     117     591 
   Size:        117     474
 
BsrDI GCAATG_nn'

Cuts at:      0     444     591 
   Size:        444     147
 
Bsu36I CC'TnA_GG

Cuts at:      0      92     591 
   Size:         92     499
 
BtsI GCAGTG_nn'

Cuts at:      0     143     591 
   Size:        143     448
 
EaeI y'GGCC_r

Cuts at:      0     225     591 
   Size:        225     366
 
EarI CTCTTCn'nnn_

Cuts at:      0     155     305     591 
   Size:        155     150     286

  Fragments arranged by size: 

                 286     155     150
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32      38      44     224     329     527     591 
   Size:         32       6       6     180     105     198      64

  Fragments arranged by size: 

                 198     180     105      64      32       6       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     249     411     591 
   Size:        249     162     180

  Fragments arranged by size: 

                 249     180     162
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     156     591 
   Size:        156     435
 
MslI CAynn'nnrTG

Cuts at:      0     448     591 
   Size:        448     143
 
MspA1I CmG'CkG

Cuts at:      0     519     591 
   Size:        519      72
 
PasI CC'CwG_GG

Cuts at:      0     252     483     591 
   Size:        252     231     108

  Fragments arranged by size: 

                 252     231     108
 
PspOMI G'GGCC_C

Cuts at:      0     248     591 
   Size:        248     343
 
PstI C_TGCA'G

Cuts at:      0     362     591 
   Size:        362     229
 
PvuII CAG'CTG

Cuts at:      0     519     591 
   Size:        519      72
 
SfcI C'TryA_G

Cuts at:      0     358     591 
   Size:        358     233
 
StuI AGG'CCT

Cuts at:      0     175     591 
   Size:        175     416
 
StyI C'CwwG_G

Cuts at:      0     101     591 
   Size:        101     490
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     442     591 
   Size:        442     149
 
TatI w'GTAC_w

Cuts at:      0     240     591 
   Size:        240     351
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI     BanI    BanII     BlpI Bme1580I
    BmgBI     BpmI   Bpu10I    BsaBI    BsaWI    BsaXI    BseYI     BsgI
    BsmBI Bsp1286I    BspMI    BsrDI   Bsu36I     BtsI     EaeI     EarI
  Eco57MI EcoO109I     FalI     MslI   MspA1I     PasI   PspOMI     PstI
    PvuII     SfcI     StuI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaHI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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