Restriction map for pF1KB8878
##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeIII      HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KspI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp509I     Van91I      XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        106  GGTACC               G^GTACC
AfaI          107  GTAC                 GT^AC
Asp718I       106  GGTACC               G^GTACC
AspI           33  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          338  CCCGGG               C^[CT]CG[GA]G
BanI          106  GGTACC               G^G[CT][GA]CC
BfaI          400  CTAG                 C^TAG
BpmI          245  CTCCAG               CTGGAG(16/14)
BsgI          496  CTGCAC               GTGCAG(16/14)
BsmFI          31  GGGAC                GGGAC(10/14)
BsoBI         338  CCCGGG               C^[CT]CG[GA]G
BspMI          71  ACCTGC               ACCTGC(4/8)
BsrDI          14  CATTGC               GCAATG(2/0)
BsrI          254  CCAGT                ACTGG(1/-1)
Cac8I         399  GCTAGC               GC[ACGT]^[ACGT]GC
EcoT14I       294  CCAAGG               C^C[AT][AT]GG
HaeII         437  AGCGCC               [GA]GCGC^[CT]
HapII         339  CCGG                 C^CGG
HpaII         339  CCGG                 C^CGG
KpnI          106  GGTACC               GGTAC^C
MaeI          400  CTAG                 C^TAG
MaeIII        217  GTCAC                ^GT[ACGT]AC
MspI          339  CCGG                 C^CGG
NheI          399  GCTAGC               G^CTAGC
NlaIII        357  CATG                 CATG^
PflFI          33  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsaI          107  GTAC                 GT^AC
SmaI          338  CCCGGG               CCC^GGG
SmlI          421  CTCAAG               C^T[CT][GA]AG
StyI          294  CCAAGG               C^C[AT][AT]GG
TaqI          434  TCGA                 T^CGA
Tsp45I        217  GTCAC                ^GT[GC]AC
TspRI          35  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I        33  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       434  TCGA                 T^CGA
XcmI           41  CCAGCTGGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         338  CCCGGG               C^CCGGG
XmaI          338  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AspHI         261  GAGCTC               G[AT]GC[AT]^C
              470  GAGCTC              
AvaII          69  GGACC                G^G[AT]CC
               81  GGTCC               
BbsI          289  GAAGAC               GAAGAC(2/6)
               87  GTCTTC              
BbvI            4  GCAGC                GCAGC(8/12)
              351  GCAGC               
BcnI          338  CCCGG                CC^[GC]GG
              339  CCGGG               
BpuAI         289  GAAGAC               GAAGAC(2/6)
               87  GTCTTC              
BsiHKAI       261  GAGCTC               G[AT]GC[AT]^C
              470  GAGCTC              
CfoI          354  GCGC                 GCG^C
              438  GCGC                
Cfr13I         69  GGACC                G^G[ACGT]CC
               81  GGTCC               
DraII          68  AGGACCT              [GA]G^G[ACGT]CC[CT]
               80  AGGTCCT             
EcoNI          72  CCTGCAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              267  CCTGGCTCAGG         
EcoO109I       68  AGGACCT              [GA]G^G[ACGT]CC[CT]
               80  AGGTCCT             
Fnu4HI          4  GCAGC                GC^[ACGT]GC
              351  GCAGC               
HhaI          354  GCGC                 GCG^C
              438  GCGC                
HinP1I        354  GCGC                 G^CGC
              438  GCGC                
ItaI            4  GCAGC                GC^[ACGT]GC
              351  GCAGC               
MspA1I          5  CAGCTG               C[AC]G^C[GT]G
               42  CAGCTG              
MwoI          172  GCTTCTCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              394  GCACAGCTAGC         
NciI          338  CCCGG                CC^[GC]GG
              339  CCGGG               
NlaIV         106  GGTACC               GG[ACGT]^[ACGT]CC
              198  GGAGCC              
PleI          462  GACTC                GAGTC(4/5)
               10  GAGTC               
PpuMI          68  AGGACCT              [GA]G^G[AT]CC[CT]
               80  AGGTCCT             
PvuII           5  CAGCTG               CAG^CTG
               42  CAGCTG              
SacI          261  GAGCTC               GAGCT^C
              470  GAGCTC              
Sau96I         69  GGACC                G^G[ACGT]CC
               81  GGTCC               
TseI            4  GCAGC                G^C[AT]GC
              351  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AluI        BanII       BmyI        BsaJI       BseRI       
BsiYI       BslI        Bsp1286I    BssKI       BstNI       
DdeI        EarI        EcoRII      HinfI       HphI        
Ksp632I     MboII       MnlI        MvaI        ScrFI       
TfiI        
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