Restriction map for pF1KA1512
##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AatII       AclI        AcsI        AgeI        ApoI        
AscI        AseI        AsnI        Asp700I     BbrPI       
BsiWI       Bsp1407I    BspDI       BspHI       BsrGI       
Bst1107I    BstZ17I     ClaI        CpoI        DraI        
EcoRI       EcoRV       FseI        HindIII     MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SnaBI       SpeI        
SspBI       SspI        SwaI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       2309  GGTACC               G^GTACC
AccI          988  GTCTAC               GT^[AC][GT]AC
AccIII       2252  TCCGGA               T^CCGGA
AflII        5656  CTTAAG               C^TTAAG
AflIII       1610  ACATGT               A^C[GA][CT]GT
AhdI          631  GACAACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I      2309  GGTACC               G^GTACC
AspEI         631  GACAACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        1149  CCTAGG               C^CTAGG
BciVI        2315  GTATCC               GTATCC(6/5)
BfrI         5656  CTTAAG               C^TTAAG
BglII        1532  AGATCT               A^GATCT
BlnI         1149  CCTAGG               C^CTAGG
BsaBI        2250  GATCCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2252  TCCGGA               T^CCGGA
BsiEI        2741  CGGCCG               CG[GA][CT]^CG
BspEI        2252  TCCGGA               T^CCGGA
BspLU11I     1610  ACATGT               A^CATGT
BssHII       3200  GCGCGC               G^CGCGC
BstBI        1552  TTCGAA               TT^CGAA
BstXI         960  CCAGTTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII       4138  CACGCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3330  GACGCAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2741  CGGCCG               C^GGCCG
Eam1105I      631  GACAACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2741  CGGCCG               C^GGCCG
Eco52I       2741  CGGCCG               C^GGCCG
EcoT22I      1746  ATGCAT               ATGCA^T
HincII        679  GTTAAC               GT[CT]^[GA]AC
HindII        679  GTTAAC               GT[CT]^[GA]AC
HpaI          679  GTTAAC               GTT^AAC
KpnI         2309  GGTACC               GGTAC^C
MamI         2250  GATCCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2252  TCCGGA               T^CCGGA
NsiI         1746  ATGCAT               ATGCA^T
NspV         1552  TTCGAA               TT^CGAA
ScaI          701  AGTACT               AGT^ACT
SfuI         1552  TTCGAA               TT^CGAA
SgrAI        2962  CGCCGGCG             C[GA]^CCGG[CT]G
SphI         5735  GCATGC               GCATG^C
TfiI          568  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AocI          840  CCTGAGG              CC^T[ACGT]AGG
             4902  CCTGAGG             
Aor51HI       443  AGCGCT               AGC^GCT
             2855  AGCGCT              
BbeI         1195  GGCGCC               GGCGC^C
             4094  GGCGCC              
BcgI         3834  CGACCTGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4272  CGAAGCAGATGC        
BsaAI         256  TACGTG               [CT]AC^GT[GA]
             1363  TACGTG              
BsrDI        2231  GCAATG               GCAATG(2/0)
             4970  GCAATG              
Bsu36I        840  CCTGAGG              CC^T[ACGT]AGG
             4902  CCTGAGG             
Eco47III      443  AGCGCT               AGC^GCT
             2855  AGCGCT              
Eco81I        840  CCTGAGG              CC^T[ACGT]AGG
             4902  CCTGAGG             
KasI         1195  GGCGCC               G^GCGCC
             4094  GGCGCC              
KspI          914  CCGCGG               CCGC^GG
             3617  CCGCGG              
MseI          680  TTAA                 T^TAA
             5657  TTAA                
NaeI         2963  GCCGGC               GCC^GGC
             5240  GCCGGC              
NarI         1195  GGCGCC               GG^CGCC
             4094  GGCGCC              
NgoMIV       2963  GCCGGC               G^CCGGC
             5240  GCCGGC              
PshAI        1947  GACCGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2772  GACGCACGTC          
SacII         914  CCGCGG               CCGC^GG
             3617  CCGCGG              
SexAI        4688  ACCAGGT              A^CC[AT]GGT
             5744  ACCTGGT             
SfoI         1195  GGCGCC               GGC^GCC
             4094  GGCGCC              
Tru9I         680  TTAA                 T^TAA
             5657  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  146 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaI        ApaLI       
AspHI       AspI        AvaI        AvaII       AviII       
BalI        BamHI       BanI        BanII       BbsI        
BbvCI       BbvI        BclI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BssSI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      SbfI        
ScrFI       SfaNI       SfcI        SfiI        SmaI        
SmlI        Sse8387I    StuI        StyI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com