Restriction map for pF1KA1512
 (Linear) (Six-base) MAPSORT of: KIAA1512.seq  Check: 5260  from: 1  to: 5949



KIAA1512.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1380    3753    5568    5949 
   Size:       1380    2373    1815     381

  Fragments arranged by size: 

                2373    1815    1380     381
 
AccI GT'mk_AC

Cuts at:      0     989    5949 
   Size:        989    4960
 
Acc65I G'GTAC_C

Cuts at:      0    2309    5949 
   Size:       2309    3640
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1140    3343    5473    5949 
   Size:       1140    2203    2130     476

  Fragments arranged by size: 

                2203    2130    1140     476
 
AfeI AGC'GCT

Cuts at:      0     445    2857    5949 
   Size:        445    2412    3092

  Fragments arranged by size: 

                3092    2412     445
 
AflII C'TTAA_G

Cuts at:      0    5656    5949 
   Size:       5656     293
 
AflIII A'CryG_T

Cuts at:      0    1610    5949 
   Size:       1610    4339
 
AhdI GACnn_n'nnGTC

Cuts at:      0     636    5949 
   Size:        636    5313
 
AleI CACnn'nnGTG

Cuts at:      0      44     113    2431    4163    5949 
   Size:         44      69    2318    1732    1786

  Fragments arranged by size: 

                2318    1786    1732      69      44
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1735    1767    2563    2595    5949 
   Size:       1735      32     796      32    3354

  Fragments arranged by size: 

                3354    1735     796      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1473    2487    2556    2763    3165    3372    3795    4152 
   Size:       1473    1014      69     207     402     207     423     357

Cuts at:   4152    4224    4462    4465    4722    5133    5166    5589    5745 
   Size:         72     238       3     257     411      33     423     156

Cuts at:   5745    5949 
   Size:        204

  Fragments arranged by size: 

                1473    1014     423     423     411     402     357     257
                 238     207     207     204     156      72      69      33
                   3
 
ApaI G_GGCC'C

Cuts at:      0    3453    3537    4797    4968    5935    5949 
   Size:       3453      84    1260     171     967      14

  Fragments arranged by size: 

                3453    1260     967     171      84      14
 
ApaLI G'TGCA_C

Cuts at:      0     196    2985    5474    5949 
   Size:        196    2789    2489     475

  Fragments arranged by size: 

                2789    2489     475     196
 
AvaI C'yCGr_G

Cuts at:      0    1340    3485    3530    5918    5949 
   Size:       1340    2145      45    2388      31

  Fragments arranged by size: 

                2388    2145    1340      45      31
 
AvrII C'CTAG_G

Cuts at:      0    1149    5949 
   Size:       1149    4800
 
BamHI G'GATC_C

Cuts at:      0    1932    2249    4826    5949 
   Size:       1932     317    2577    1123

  Fragments arranged by size: 

                2577    1932    1123     317
 
BanI G'GyrC_C

Cuts at:      0      46     234     775    1195    1893    2309    4094    4579 
   Size:         46     188     541     420     698     416    1785     485

Cuts at:   4579    5949 
   Size:       1370

  Fragments arranged by size: 

                1785    1370     698     541     485     420     416     188
                  46
 
BanII G_rGCy'C

Cuts at:      0    1121    1413    2017    2033    2389    2474    2996    3453 
   Size:       1121     292     604      16     356      85     522     457

Cuts at:   3453    3537    4589    4797    4841    4968    5147    5525    5696 
   Size:         84    1052     208      44     127     179     378     171

Cuts at:   5696    5935    5949 
   Size:        239      14

  Fragments arranged by size: 

                1121    1052     604     522     457     378     356     292
                 239     208     179     171     127      85      84      44
                  16      14
 
BbeI G_GCGC'C

Cuts at:      0    1199    4098    5949 
   Size:       1199    2899    1851

  Fragments arranged by size: 

                2899    1851    1199
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1653    3437    5503    5949 
   Size:       1653    1784    2066     446

  Fragments arranged by size: 

                2066    1784    1653     446
 
BbvCI CC'TCA_GC

Cuts at:      0    2681    4199    4455    5399    5949 
   Size:       2681    1518     256     944     550

  Fragments arranged by size: 

                2681    1518     944     550     256
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3823    3857    4261    4295    5949 
   Size:       3823      34     404      34    1654

  Fragments arranged by size: 

                3823    1654     404      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2326    5949 
   Size:       2326    3623
 
BclI T'GATC_A

Cuts at:      0     647    2870    4037    5949 
   Size:        647    2223    1167    1912

  Fragments arranged by size: 

                2223    1912    1167     647
 
BfrBI ATG'CAT

Cuts at:      0    1748    5949 
   Size:       1748    4201
 
BglI GCCn_nnn'nGGC

Cuts at:      0     817    2704    3712    4147    4249    5059    5416    5440 
   Size:        817    1887    1008     435     102     810     357      24

Cuts at:   5440    5949 
   Size:        509

  Fragments arranged by size: 

                1887    1008     817     810     509     435     357     102
                  24
 
BglII A'GATC_T

Cuts at:      0    1532    5949 
   Size:       1532    4417
 
BlpI GC'TnA_GC

Cuts at:      0    2825    3406    3415    3583    3913    4048    4211    5141 
   Size:       2825     581       9     168     330     135     163     930

Cuts at:   5141    5356    5618    5949 
   Size:        215     262     331

  Fragments arranged by size: 

                2825     930     581     331     330     262     215     168
                 163     135       9
 
Bme1580I G_kGCm'C

Cuts at:      0     200     778    2989    3453    3537    4131    4503    4582 
   Size:        200     578    2211     464      84     594     372      79

Cuts at:   4582    4797    4968    5478    5913    5935    5949 
   Size:        215     171     510     435      22      14

  Fragments arranged by size: 

                2211     594     578     510     464     435     372     215
                 200     171      84      79      22      14
 
BmgBI CAC'GTC

Cuts at:      0    2778    5949 
   Size:       2778    3171
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     107     511    1665    1726    2321    2403    4248    5949 
   Size:        107     404    1154      61     595      82    1845    1701

  Fragments arranged by size: 

                1845    1701    1154     595     404     107      82      61
            
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     403     958    1642    1756    1939    2263    2785 
   Size:         67     336     555     684     114     183     324     522

Cuts at:   2785    2911    2944    2995    3037    3247    3280    3331    3394 
   Size:        126      33      51      42     210      33      51      63

Cuts at:   3394    3532    3595    3616    3700    3817    4324    4456    4462 
   Size:        138      63      21      84     117     507     132       6

Cuts at:   4462    4525    4567    4651    4744    4798    4819    4825    4855 
   Size:         63      42      84      93      54      21       6      30

Cuts at:   4855    5041    5188    5221    5242    5356    5509    5524    5611 
   Size:        186     147      33      21     114     153      15      87

Cuts at:   5611    5632    5949 
   Size:         21     317

  Fragments arranged by size: 

                 684     555     522     507     336     324     317     210
                 186     183     153     147     138     132     126     117
                 114     114      93      87      84      84      67      63
                  63      63      54      51      51      42      42      33
                  33      33      30      21      21      21      21      15
                   6       6
 
Bpu10I CC'TnA_GC

Cuts at:      0    2681    4199    4455    5399    5949 
   Size:       2681    1518     256     944     550

  Fragments arranged by size: 

                2681    1518     944     550     256
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     802    2563    2980    3341    3370    4624    4972    5080 
   Size:        802    1761     417     361      29    1254     348     108

Cuts at:   5080    5557    5949 
   Size:        477     392

  Fragments arranged by size: 

                1761    1254     802     477     417     392     361     348
                 108      29
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     301    1939    4804    5949 
   Size:        301    1638    2865    1145

  Fragments arranged by size: 

                2865    1638    1145     301
 
BsaAI yAC'GTr

Cuts at:      0     258    1365    5949 
   Size:        258    1107    4584

  Fragments arranged by size: 

                4584    1107     258
 
BsaBI GATnn'nnATC

Cuts at:      0    2254    5949 
   Size:       2254    3695
 
BsaHI Gr'CG_yC

Cuts at:      0     251    1082    1196    1769    2459    4095    5924    5949 
   Size:        251     831     114     573     690    1636    1829      25

  Fragments arranged by size: 

                1829    1636     831     690     573     251     114      25
            
 
BsaWI w'CCGG_w

Cuts at:      0     652    2252    4865    5949 
   Size:        652    1600    2613    1084

  Fragments arranged by size: 

                2613    1600    1084     652
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2968    2998    4015    4045    5949 
   Size:       2968      30    1017      30    1904

  Fragments arranged by size: 

                2968    1904    1017      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88      91     846    1129    1258    1309    1645    1753 
   Size:         88       3     755     283     129      51     336     108

Cuts at:   1753    2698    2701    2782    2866    2947    2950    3199    3391 
   Size:        945       3      81      84      81       3     249     192

Cuts at:   3391    3508    3520    3592    3613    3697    3952    4036    4120 
   Size:        117      12      72      21      84     255      84      84

Cuts at:   4120    4204    4207    4275    4312    4321    4363    4540    4543 
   Size:         84       3      68      37       9      42     177       3

Cuts at:   4543    4627    4681    4795    4822    4903    5116    5137    5218 
   Size:         84      54     114      27      81     213      21      81

Cuts at:   5218    5386    5416    5461    5554    5725    5809    5881    5949 
   Size:        168      30      45      93     171      84      72      68

  Fragments arranged by size: 

                 945     755     336     283     255     249     213     192
                 177     171     168     129     117     114     108      93
                  88      84      84      84      84      84      84      84
                  81      81      81      81      72      72      68      68
                  54      51      45      42      37      30      27      21
                  21      12       9       3       3       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0    1522    2090    2382    2446    2559    3657    3753    5031 
   Size:       1522     568     292      64     113    1098      96    1278

Cuts at:   5031    5351    5843    5949 
   Size:        320     492     106

  Fragments arranged by size: 

                1522    1278    1098     568     492     320     292     113
                 106      96      64
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    1189    2080    2905    3575    3899    4165    4757 
   Size:        412     777     891     825     670     324     266     592

Cuts at:   4757    5363    5602    5949 
   Size:        606     239     347

  Fragments arranged by size: 

                 891     825     777     670     606     592     412     347
                 324     266     239
 
BsiEI CG_ry'CG

Cuts at:      0    2744    5949 
   Size:       2744    3205
 
BsiHKAI G_wGCw'C

Cuts at:      0     200    1121    2474    2989    2996    4381    4589    4841 
   Size:        200     921    1353     515       7    1385     208     252

Cuts at:   4841    5147    5478    5525    5696    5949 
   Size:        306     331      47     171     253

  Fragments arranged by size: 

                1385    1353     921     515     331     306     253     252
                 208     200     171      47       7
 
BsmI GAATG_Cn'

Cuts at:      0     881    1750    3256    5295    5949 
   Size:        881     869    1506    2039     654

  Fragments arranged by size: 

                2039    1506     881     869     654
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     722    2764    4627    5949 
   Size:        722    2042    1863    1322

  Fragments arranged by size: 

                2042    1863    1322     722
 
Bsp1286I G_dGCh'C

Cuts at:      0     200     778    1121    1413    2017    2033    2389    2474 
   Size:        200     578     343     292     604      16     356      85

Cuts at:   2474    2989    2996    3453    3537    4131    4381    4503    4582 
   Size:        515       7     457      84     594     250     122      79

Cuts at:   4582    4589    4797    4841    4968    5147    5478    5525    5696 
   Size:          7     208      44     127     179     331      47     171

Cuts at:   5696    5913    5935    5949 
   Size:        217      22      14

  Fragments arranged by size: 

                 604     594     578     515     457     356     343     331
                 292     250     217     208     200     179     171     127
                 122      85      84      79      47      44      22      16
                  14       7       7
 
BspEI T'CCGG_A

Cuts at:      0    2252    5949 
   Size:       2252    3697
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     443    1380    1691    2138    3182    3753    4667    5568 
   Size:        443     937     311     447    1044     571     914     901

Cuts at:   5568    5949 
   Size:        381

  Fragments arranged by size: 

                1044     937     914     901     571     447     443     381
                 311
 
BsrBI CCG'CTC

Cuts at:      0     563    2919    3294    3522    3591    4446    5360    5871 
   Size:        563    2356     375     228      69     855     914     511

Cuts at:   5871    5949 
   Size:         78

  Fragments arranged by size: 

                2356     914     855     563     511     375     228      78
                  69
 
BsrDI GCAATG_nn'

Cuts at:      0    2238    4977    5949 
   Size:       2238    2739     972

  Fragments arranged by size: 

                2739    2238     972
 
BsrFI r'CCGG_y

Cuts at:      0     667    1433    2963    5240    5949 
   Size:        667     766    1530    2277     709

  Fragments arranged by size: 

                2277    1530     766     709     667
 
BssHII G'CGCG_C

Cuts at:      0    3200    5949 
   Size:       3200    2749
 
BssSI C'ACGA_G

Cuts at:      0    3700    4186    4609    5149    5848    5949 
   Size:       3700     486     423     540     699     101

  Fragments arranged by size: 

                3700     699     540     486     423     101
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     435    1032    3783    4152    4224    4465    5514    5949 
   Size:        435     597    2751     369      72     241    1049     435

  Fragments arranged by size: 

                2751    1049     597     435     435     369     241      72
            
 
BstBI TT'CG_AA

Cuts at:      0    1553    5949 
   Size:       1553    4396
 
BstEII G'GTnAC_C

Cuts at:      0    3169    3316    5005    5949 
   Size:       3169     147    1689     944

  Fragments arranged by size: 

                3169    1689     944     147
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     967    5949 
   Size:        967    4982
 
BstYI r'GATC_y

Cuts at:      0     998    1532    1932    2249    2255    4763    4826    5949 
   Size:        998     534     400     317       6    2508      63    1123

  Fragments arranged by size: 

                2508    1123     998     534     400     317      63       6
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     841    4903    5949 
   Size:        841    4062    1046

  Fragments arranged by size: 

                4062    1046     841
 
BtgI C'CryG_G

Cuts at:      0      42     208     302     812     914    1091    1139    1949 
   Size:         42     166      94     510     102     177      48     810

Cuts at:   1949    2523    3617    4053    4569    5562    5949 
   Size:        574    1094     436     516     993     387

  Fragments arranged by size: 

                1094     993     810     574     516     510     436     387
                 177     166     102      94      48      42
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1107    1503    5949 
   Size:       1107     396    4446

  Fragments arranged by size: 

                4446    1107     396
 
BtsI GCAGTG_nn'

Cuts at:      0     119    1314    3718    4327    5949 
   Size:        119    1195    2404     609    1622

  Fragments arranged by size: 

                2404    1622    1195     609     119
 
DraIII CAC_nnn'GTG

Cuts at:      0    4143    5949 
   Size:       4143    1806
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3336    5949 
   Size:       3336    2613
 
EaeI y'GGCC_r

Cuts at:      0     392    1415    2421    2489    2526    2741    2954    3029 
   Size:        392    1023    1006      68      37     215     213      75

Cuts at:   3029    4067    4605    5432    5441    5825    5949 
   Size:       1038     538     827       9     384     124

  Fragments arranged by size: 

                1038    1023    1006     827     538     392     384     215
                 213     124      75      68      37       9
 
EagI C'GGCC_G

Cuts at:      0    2741    5949 
   Size:       2741    3208
 
EarI CTCTTCn'nnn_

Cuts at:      0     487    2462    3080    3497    3802    4079    4594    4702 
   Size:        487    1975     618     417     305     277     515     108

Cuts at:   4702    4750    5949 
   Size:         48    1199

  Fragments arranged by size: 

                1975    1199     618     515     487     417     305     277
                 108      48
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     157     610    1267    3505    3667    3751    3763    3973 
   Size:        157     453     657    2238     162      84      12     210

Cuts at:   3973    5356    5815    5899    5949 
   Size:       1383     459      84      50

  Fragments arranged by size: 

                2238    1383     657     459     453     210     162     157
                  84      84      50      12
 
EcoICRI GAG'CTC

Cuts at:      0    1119    2472    2994    4587    4839    5145    5523    5694 
   Size:       1119    1353     522    1593     252     306     378     171

Cuts at:   5694    5949 
   Size:        255

  Fragments arranged by size: 

                1593    1353    1119     522     378     306     255     252
                 171
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     403     958    1140    1642    1756    1939    2263 
   Size:         67     336     555     182     502     114     183     324

Cuts at:   2263    2785    2911    2944    2995    3037    3247    3280    3331 
   Size:        522     126      33      51      42     210      33      51

Cuts at:   3331    3343    3394    3532    3595    3616    3700    3817    4324 
   Size:         12      51     138      63      21      84     117     507

Cuts at:   4324    4456    4462    4525    4567    4651    4744    4798    4819 
   Size:        132       6      63      42      84      93      54      21

Cuts at:   4819    4825    4855    5041    5188    5221    5242    5356    5473 
   Size:          6      30     186     147      33      21     114     117

Cuts at:   5473    5509    5524    5611    5632    5949 
   Size:         36      15      87      21     317

  Fragments arranged by size: 

                 555     522     507     502     336     324     317     210
                 186     183     182     147     138     132     126     117
                 117     114     114      93      87      84      84      67
                  63      63      54      51      51      51      42      42
                  36      33      33      33      30      21      21      21
                  21      15      12       6       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     169     412    1738    2491    3262    4018    4663    5224 
   Size:        169     243    1326     753     771     756     645     561

Cuts at:   5224    5539    5949 
   Size:        315     410

  Fragments arranged by size: 

                1326     771     756     753     645     561     410     315
                 243     169
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1314    2238    3162    3243    3449    4062    4793    4794 
   Size:       1314     924     924      81     206     613     731       1

Cuts at:   4794    5652    5742    5932    5949 
   Size:        858      90     190      17

  Fragments arranged by size: 

                1314     924     924     858     731     613     206     190
                  90      81      17       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2454    5949 
   Size:       2454    3495
 
FspI TGC'GCA

Cuts at:      0     622    3280    3820    5668    5949 
   Size:        622    2658     540    1848     281

  Fragments arranged by size: 

                2658    1848     622     540     281
 
HaeII r_GCGC'y

Cuts at:      0     447     561    1199    2487    2556    2734    2757    2859 
   Size:        447     114     638    1288      69     178      23     102

Cuts at:   2859    3694    4098    4744    4981    5175    5283    5682    5787 
   Size:        835     404     646     237     194     108     399     105

Cuts at:   5787    5949 
   Size:        162

  Fragments arranged by size: 

                1288     835     646     638     447     404     399     237
                 194     178     162     114     108     105     102      69
                  23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291     622     654     841     873    5949 
   Size:        259      32     331      32     187      32    5076

  Fragments arranged by size: 

                5076     331     259     187      32      32      32
 
HincII GTy'rAC

Cuts at:      0     681    5949 
   Size:        681    5268
 
HpaI GTT'AAC

Cuts at:      0     681    5949 
   Size:        681    5268
 
KasI G'GCGC_C

Cuts at:      0    1195    4094    5949 
   Size:       1195    2899    1855

  Fragments arranged by size: 

                2899    1855    1195
 
KpnI G_GTAC'C

Cuts at:      0    2313    5949 
   Size:       2313    3636
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     257    4367    5949 
   Size:        257    4110    1582

  Fragments arranged by size: 

                4110    1582     257
 
MscI TGG'CCA

Cuts at:      0     394    1417    2423    2491    3031    5827    5949 
   Size:        394    1023    1006      68     540    2796     122

  Fragments arranged by size: 

                2796    1023    1006     540     394     122      68
 
MslI CAynn'nnrTG

Cuts at:      0      44     113     124     394     584    1090    1615    2350 
   Size:         44      69      11     270     190     506     525     735

Cuts at:   2350    2431    3826    4163    5949 
   Size:         81    1395     337    1786

  Fragments arranged by size: 

                1786    1395     735     525     506     337     270     190
                  81      69      44      11
 
MspA1I CmG'CkG

Cuts at:      0     455     916    1239    1560    2094    2159    2223    2307 
   Size:        455     461     323     321     534      65      64      84

Cuts at:   2307    2661    2817    2889    3372    3414    3619    3780    3948 
   Size:        354     156      72     483      42     205     161     168

Cuts at:   3948    3954    4047    4221    4462    4485    4539    4833    5031 
   Size:          6      93     174     241      23      54     294     198

Cuts at:   5031    5130    5140    5355    5949 
   Size:         99      10     215     594

  Fragments arranged by size: 

                 594     534     483     461     455     354     323     321
                 294     241     215     205     198     174     168     161
                 156      99      93      84      72      65      64      54
                  42      23      10       6
 
NaeI GCC'GGC

Cuts at:      0    2965    5242    5949 
   Size:       2965    2277     707

  Fragments arranged by size: 

                2965    2277     707
 
NarI GG'CG_CC

Cuts at:      0    1196    4095    5949 
   Size:       1196    2899    1854

  Fragments arranged by size: 

                2899    1854    1196
 
NcoI C'CATG_G

Cuts at:      0     208     812    1139    5949 
   Size:        208     604     327    4810

  Fragments arranged by size: 

                4810     604     327     208
 
NgoMIV G'CCGG_C

Cuts at:      0    2963    5240    5949 
   Size:       2963    2277     709

  Fragments arranged by size: 

                2963    2277     709
 
NsiI A_TGCA'T

Cuts at:      0    1750    5949 
   Size:       1750    4199
 
NspI r_CATG'y

Cuts at:      0     618    1035    1614    1752    5739    5949 
   Size:        618     417     579     138    3987     210

  Fragments arranged by size: 

                3987     618     579     417     210     138
 
PasI CC'CwG_GG

Cuts at:      0     723    2188    3165    5928    5949 
   Size:        723    1465     977    2763      21

  Fragments arranged by size: 

                2763    1465     977     723      21
 
PciI A'CATG_T

Cuts at:      0    1610    5949 
   Size:       1610    4339
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     756    1140    1383    1473    2487    2591    2763    4080 
   Size:        756     384     243      90    1014     104     172    1317

Cuts at:   4080    4722    5949 
   Size:        642    1227

  Fragments arranged by size: 

                1317    1227    1014     756     642     384     243     172
                 104      90
 
PfoI T'CCnGG_A

Cuts at:      0     935    1733    2240    2336    2462    4715    5949 
   Size:        935     798     507      96     126    2253    1234

  Fragments arranged by size: 

                2253    1234     935     798     507     126      96
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     403     435    5949 
   Size:        403      32    5514

  Fragments arranged by size: 

                5514     403      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1314    2238    3243    5742    5949 
   Size:       1314     924    1005    2499     207

  Fragments arranged by size: 

                2499    1314    1005     924     207
 
PshAI GACnn'nnGTC

Cuts at:      0    1951    2776    5949 
   Size:       1951     825    3173

  Fragments arranged by size: 

                3173    1951     825
 
PspOMI G'GGCC_C

Cuts at:      0    3449    3533    4793    4964    5931    5949 
   Size:       3449      84    1260     171     967      18

  Fragments arranged by size: 

                3449    1260     967     171      84      18
 
PstI C_TGCA'G

Cuts at:      0     353    1028    1075    1688    2513    2816    2822    3179 
   Size:        353     675      47     613     825     303       6     357

Cuts at:   3179    3482    3779    3947    4220    4340    4664    4940    5030 
   Size:        303     297     168     273     120     324     276      90

Cuts at:   5030    5762    5949 
   Size:        732     187

  Fragments arranged by size: 

                 825     732     675     613     357     353     324     303
                 303     297     276     273     187     168     120      90
                  47       6
 
PvuII CAG'CTG

Cuts at:      0    1560    2094    2223    2817    2889    3372    3414    3954 
   Size:       1560     534     129     594      72     483      42     540

Cuts at:   3954    4047    4462    4833    5031    5140    5355    5949 
   Size:         93     415     371     198     109     215     594

  Fragments arranged by size: 

                1560     594     594     540     534     483     415     371
                 215     198     129     109      93      72      42
 
SacI G_AGCT'C

Cuts at:      0    1121    2474    2996    4589    4841    5147    5525    5696 
   Size:       1121    1353     522    1593     252     306     378     171

Cuts at:   5696    5949 
   Size:        253

  Fragments arranged by size: 

                1593    1353    1121     522     378     306     253     252
                 171
 
SacII CC_GC'GG

Cuts at:      0     917    3620    5949 
   Size:        917    2703    2329

  Fragments arranged by size: 

                2703    2329     917
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2462    4594    4702    5949 
   Size:       2462    2132     108    1247

  Fragments arranged by size: 

                2462    2132    1247     108
 
SbfI CC_TGCA'GG

Cuts at:      0    3179    4664    4940    5949 
   Size:       3179    1485     276    1009

  Fragments arranged by size: 

                3179    1485    1009     276
 
ScaI AGT'ACT

Cuts at:      0     703    5949 
   Size:        703    5246
 
SexAI A'CCwGG_T

Cuts at:      0    4688    5744    5949 
   Size:       4688    1056     205

  Fragments arranged by size: 

                4688    1056     205
 
SfcI C'TryA_G

Cuts at:      0     349    1024    1071    1684    2509    2812    2818    3175 
   Size:        349     675      47     613     825     303       6     357

Cuts at:   3175    3478    3775    3943    3985    4216    4336    4408    4660 
   Size:        303     297     168      42     231     120      72     252

Cuts at:   4660    4936    5026    5758    5949 
   Size:        276      90     732     191

  Fragments arranged by size: 

                 825     732     675     613     357     349     303     303
                 297     276     252     231     191     168     120      90
                  72      47      42       6
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    5059    5416    5440    5949 
   Size:       5059     357      24     509

  Fragments arranged by size: 

                5059     509     357      24
 
SfoI GGC'GCC

Cuts at:      0    1197    4096    5949 
   Size:       1197    2899    1853

  Fragments arranged by size: 

                2899    1853    1197
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2963    5949 
   Size:       2963    2986
 
SmaI CCC'GGG

Cuts at:      0    1342    3532    5920    5949 
   Size:       1342    2190    2388      29

  Fragments arranged by size: 

                2388    2190    1342      29
 
SmlI C'TyrA_G

Cuts at:      0     781    2542    2995    3356    3385    4639    4987    5095 
   Size:        781    1761     453     361      29    1254     348     108

Cuts at:   5095    5536    5656    5949 
   Size:        441     120     293

  Fragments arranged by size: 

                1761    1254     781     453     441     361     348     293
                 120     108      29
 
SphI G_CATG'C

Cuts at:      0    5739    5949 
   Size:       5739     210
 
SrfI GCCC'GGGC

Cuts at:      0    3532    5949 
   Size:       3532    2417
 
StuI AGG'CCT

Cuts at:      0     418     479     820    1328    1762    4942    5312    5949 
   Size:        418      61     341     508     434    3180     370     637

  Fragments arranged by size: 

                3180     637     508     434     418     370     341      61
            
 
StyI C'CwwG_G

Cuts at:      0      64     208     812    1139    1149    1370    1418    1757 
   Size:         64     144     604     327      10     221      48     339

Cuts at:   1757    2265    2429    2492    2879    3125    3869    4232    5597 
   Size:        508     164      63     387     246     744     363    1365

Cuts at:   5597    5949 
   Size:        352

  Fragments arranged by size: 

                1365     744     604     508     387     363     352     339
                 327     246     221     164     144      64      63      48
                  10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     222    1830    2422    2803    3157    4309    5949 
   Size:        222    1608     592     381     354    1152    1640

  Fragments arranged by size: 

                1640    1608    1152     592     381     354     222
 
TatI w'GTAC_w

Cuts at:      0     701    1634    5949 
   Size:        701     933    4315

  Fragments arranged by size: 

                4315     933     701
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1706    1738    1805    1837    2586    2618    2968    3000 
   Size:       1706      32      67      32     749      32     350      32

Cuts at:   3000    4015    4047    5949 
   Size:       1015      32    1902

  Fragments arranged by size: 

                1902    1706    1015     749     350      67      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1657    2085    4416    5949 
   Size:       1657     428    2331    1533

  Fragments arranged by size: 

                2331    1657    1533     428
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     126     810    2416    2886    3222    3306    3930    4128 
   Size:        126     684    1606     470     336      84     624     198

Cuts at:   4128    4245    4320    4740    5820    5949 
   Size:        117      75     420    1080     129

  Fragments arranged by size: 

                1606    1080     684     624     470     420     336     198
                 129     126     117      84      75
 
XmaI C'CCGG_G

Cuts at:      0    1340    3530    5918    5949 
   Size:       1340    2190    2388      31

  Fragments arranged by size: 

                2388    2190    1340      31
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI    AflII   AflIII     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI    SacII
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI    SgrAI
     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AgeI     AlfI     ApoI     AscI     AseI    AsiSI
     BaeI     BmtI     BplI    BsiWI    BspHI    BsrGI  BstZ17I     ClaI
    CspCI     DraI    EcoRI    EcoRV     FseI    FspAI  HindIII     MfeI
     MluI     NdeI     NheI     NotI     NruI     PacI     PmeI     PmlI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SgfI
    SnaBI     SpeI     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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