Restriction map for pF1KB6498
 (Circular) (Six-base) MAPSORT of: pF1KB6498.seq  Check: 5122  from: 1  to: 3296



pF1KB6498  3296 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    157     157 
   Size:       3296
 
AccI GT'mk_AC

Cuts at:    334     334 
   Size:       3296
 
Acc65I G'GTAC_C

Cuts at:    312     312 
   Size:       3296
 
AclI AA'CG_TT

Cuts at:   3136    3136 
   Size:       3296
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1121    1553    2300    2568    1121 
   Size:        432     747     268    1849

  Fragments arranged by size: 

                1849     747     432     268
 
AflIII A'CryG_T

Cuts at:    648    1752    2729     648 
   Size:       1104     977    1215

  Fragments arranged by size: 

                1215    1104     977
 
AgeI A'CCGG_T

Cuts at:   1684    1684 
   Size:       3296
 
AlwNI CAG_nnn'CTG

Cuts at:    284     601    2168     284 
   Size:        317    1567    1412

  Fragments arranged by size: 

                1567    1412     317
 
ApaLI G'TGCA_C

Cuts at:   2066    2066 
   Size:       3296
 
ApoI r'AATT_y

Cuts at:    300    2649    2905     300 
   Size:       2349     256     691

  Fragments arranged by size: 

                2349     691     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3296
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3296
 
AvaI C'yCGr_G

Cuts at:    316     316 
   Size:       3296
 
BamHI G'GATC_C

Cuts at:    111     321     111 
   Size:        210    3086
 
BanI G'GyrC_C

Cuts at:    312     726     974    1009     312 
   Size:        414     248      35    2599

  Fragments arranged by size: 

                2599     414     248      35
 
BanII G_rGCy'C

Cuts at:    310    1340     310 
   Size:       1030    2266
 
BbeI G_GCGC'C

Cuts at:    730     978     730 
   Size:        248    3048
 
BciVI GTATCCnnnnn_n'

Cuts at:   1194    1955    3296    1194 
   Size:        761    1341    1194

  Fragments arranged by size: 

                1341    1194     761
 
BclI T'GATC_A

Cuts at:    816     816 
   Size:       3296
 
BglI GCCn_nnn'nGGC

Cuts at:    224     224 
   Size:       3296
 
BglII A'GATC_T

Cuts at:    811    2848     811 
   Size:       2037    1259
 
BlpI GC'TnA_GC

Cuts at:    408     408 
   Size:       3296
 
Bme1580I G_kGCm'C

Cuts at:    243     921    1014    2070     243 
   Size:        678      93    1056    1469

  Fragments arranged by size: 

                1469    1056     678      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    508     589     924    3068     508 
   Size:         81     335    2144     736

  Fragments arranged by size: 

                2144     736     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1663    1663 
   Size:       3296
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    472    1351    1843    2141     472 
   Size:        879     492     298    1627

  Fragments arranged by size: 

                1627     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3000    3000 
   Size:       3296
 
BsaAI yAC'GTr

Cuts at:    649     649 
   Size:       3296
 
BsaBI GATnn'nnATC

Cuts at:      6     810    2847       6 
   Size:        804    2037     455

  Fragments arranged by size: 

                2037     804     455
 
BsaHI Gr'CG_yC

Cuts at:    265     727     975    1677    3173     265 
   Size:        462     248     702    1496     388

  Fragments arranged by size: 

                1496     702     462     388     248
 
BsaWI w'CCGG_w

Cuts at:    485    1006    1684    1958    2105    2516     485 
   Size:        521     678     274     147     411    1265

  Fragments arranged by size: 

                1265     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    721    1479    2056    2552     721 
   Size:        758     577     496    1465

  Fragments arranged by size: 

                1465     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    129     297     129 
   Size:        168    3128
 
BsiEI CG_ry'CG

Cuts at:    104     838     884    2092     104 
   Size:        734      46    1208    1308

  Fragments arranged by size: 

                1308    1208     734      46
 
BsiHKAI G_wGCw'C

Cuts at:    310    1088    1278    2070     310 
   Size:        778     190     792    1536

  Fragments arranged by size: 

                1536     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    243     310     921    1014    1088    1278    1340    2070     243 
   Size:         67     611      93      74     190      62     730    1469

  Fragments arranged by size: 

                1469     730     611     190      93      74      67      62
            
 
BspEI T'CCGG_A

Cuts at:   2516    2516 
   Size:       3296
 
BspHI T'CATG_A

Cuts at:   2472    2472 
   Size:       3296
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    157     346     560     862    1243     157 
   Size:        189     214     302     381    2210

  Fragments arranged by size: 

                2210     381     302     214     189
 
BsrBI CCG'CTC

Cuts at:     50    1588    1642    3126    3291      50 
   Size:       1538      54    1484     165      55

  Fragments arranged by size: 

                1538    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1208    1208 
   Size:       3296
 
BsrFI r'CCGG_y

Cuts at:    133     154    1294    1475    1684     133 
   Size:         21    1140     181     209    1745

  Fragments arranged by size: 

                1745    1140     209     181      21
 
BssSI C'ACGA_G

Cuts at:   1567    1925    1567 
   Size:        358    2938
 
BstAPI GCAn_nnn'nTGC

Cuts at:    176     191     284     673     176 
   Size:         15      93     389    2799

  Fragments arranged by size: 

                2799     389      93      15
 
BstBI TT'CG_AA

Cuts at:   1657    1657 
   Size:       3296
 
BstYI r'GATC_y

Cuts at:      1     111     321     788     811    1146    1392    2404    2490 
   Size:        110     210     467      23     335     246    1012      86

Cuts at:   2490    2502    2848       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     246     210     110
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3296
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1246    1427    1590    1246 
   Size:        181     163    2952

  Fragments arranged by size: 

                2952     181     163
 
BtsI GCAGTG_nn'

Cuts at:    539     539 
   Size:       3296
 
DraI TTT'AAA

Cuts at:    295     295 
   Size:       3296
 
DrdI GACnn_nn'nnGTC

Cuts at:   1002    1860    1002 
   Size:        858    2438
 
EaeI y'GGCC_r

Cuts at:    881    1055    1446    1473    2818     881 
   Size:        174     391      27    1345    1359

  Fragments arranged by size: 

                1359    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    881     881 
   Size:       3296
 
EarI CTCTTCn'nnn_

Cuts at:   1319    1529    1319 
   Size:        210    3086
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1504    1814    1960    3106    1504 
   Size:        310     146    1146    1694

  Fragments arranged by size: 

                1694    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    308     308 
   Size:       3296
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1121    1553    2300    2568    1121 
   Size:        432     747     268    1849

  Fragments arranged by size: 

                1849     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    435     435 
   Size:       3296
 
EcoRI G'AATT_C

Cuts at:    300     300 
   Size:       3296
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1315    1315 
   Size:       3296
 
FspI TGC'GCA

Cuts at:   1077    1077 
   Size:       3296
 
HaeII r_GCGC'y

Cuts at:    730     978    2000    2979     730 
   Size:        248    1022     979    1047

  Fragments arranged by size: 

                1047    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1138    1168    1170    1200    1138 
   Size:         30       2      30    3234

  Fragments arranged by size: 

                3234      30      30       2
 
HincII GTy'rAC

Cuts at:    335     335 
   Size:       3296
 
KasI G'GCGC_C

Cuts at:    726     974     726 
   Size:        248    3048
 
KpnI G_GTAC'C

Cuts at:    316     316 
   Size:       3296
 
MluI A'CGCG_T

Cuts at:   2729    2729 
   Size:       3296
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    248    1967    2151     248 
   Size:       1719     184    1393

  Fragments arranged by size: 

                1719    1393     184
 
MscI TGG'CCA

Cuts at:   1057    1057 
   Size:       3296
 
MslI CAynn'nnrTG

Cuts at:   1285    1412    1285 
   Size:        127    3169
 
MspA1I CmG'CkG

Cuts at:    281     407     721    1081    2094    2339     281 
   Size:        126     314     360    1013     245    1238

  Fragments arranged by size: 

                1238    1013     360     314     245     126
 
NaeI GCC'GGC

Cuts at:    156    1477     156 
   Size:       1321    1975
 
NarI GG'CG_CC

Cuts at:    727     975     727 
   Size:        248    3048
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3296
 
NgoMIV G'CCGG_C

Cuts at:    154    1475     154 
   Size:       1321    1975
 
NspI r_CATG'y

Cuts at:    349     849    1756     349 
   Size:        500     907    1889

  Fragments arranged by size: 

                1889     907     500
 
PasI CC'CwG_GG

Cuts at:    243     243 
   Size:       3296
 
PciI A'CATG_T

Cuts at:   1752    1752 
   Size:       3296
 
PmeI GTTT'AAAC

Cuts at:    295     295 
   Size:       3296
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2435    2467    2435 
   Size:         32    3264
 
PstI C_TGCA'G

Cuts at:    343    1028     343 
   Size:        685    2611
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3296
 
PvuII CAG'CTG

Cuts at:    281     721    1081     281 
   Size:        440     360    2496

  Fragments arranged by size: 

                2496     440     360
 
RsrII CG'GwC_CG

Cuts at:   1491    1491 
   Size:       3296
 
SacI G_AGCT'C

Cuts at:    310     310 
   Size:       3296
 
SalI G'TCGA_C

Cuts at:    333     333 
   Size:       3296
 
SapI GCTCTTCn'nnn_

Cuts at:   1319    1529    1319 
   Size:        210    3086
 
SbfI CC_TGCA'GG

Cuts at:    343     343 
   Size:       3296
 
ScaI AGT'ACT

Cuts at:   2813    2813 
   Size:       3296
 
SfcI C'TryA_G

Cuts at:     33     339    1024    2017    2208      33 
   Size:        306     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     306     191
 
SfoI GGC'GCC

Cuts at:    728     976     728 
   Size:        248    3048
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3296
 
SgrAI Cr'CCGG_yG

Cuts at:    133     133 
   Size:       3296
 
SmaI CCC'GGG

Cuts at:    318     318 
   Size:       3296
 
SmlI C'TyrA_G

Cuts at:    451    1366    1858    2120     451 
   Size:        915     492     262    1627

  Fragments arranged by size: 

                1627     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3296
 
SphI G_CATG'C

Cuts at:    349     849     349 
   Size:        500    2796
 
StyI C'CwwG_G

Cuts at:    107     430     784     107 
   Size:        323     354    2619

  Fragments arranged by size: 

                2619     354     323
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    879    1012    1471    1680     879 
   Size:        133     459     209    2495

  Fragments arranged by size: 

                2495     459     209     133
 
TatI w'GTAC_w

Cuts at:   2811    2811 
   Size:       3296
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2813    2845    2813 
   Size:         32    3264
 
Tth111I GACn'n_nGTC

Cuts at:   1093    1093 
   Size:       3296
 
XbaI T'CTAG_A

Cuts at:    327     327 
   Size:       3296
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    791     791 
   Size:       3296
 
XmaI C'CCGG_G

Cuts at:    316     316 
   Size:       3296
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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