Restriction map for pF1KB8940
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiHKAI     BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DpnII       DraI        DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NdeI        NdeII       NheI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   14 enzymes) #
###################################

AvaII         407  GGACC                G^G[AT]CC
BsmBI         619  GAGACG               CGTCTC(1/5)
BspMI         624  GCAGGT               ACCTGC(4/8)
BsrBI         322  CCGCTC               CCGCTC(-3/-3)
BsrI          267  ACTGG                ACTGG(1/-1)
BstF5I        416  GGATG                GGATG(2/0)
DraIII         14  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          309  CTCTTC               CTCTTC(1/4)
FokI          416  GGATG                GGATG(9/13)
Ksp632I       309  CTCTTC               CTCTTC(1/4)
NcoI          107  CCATGG               C^CATGG
NspI          875  GCATGT               [GA]CATG^[CT]
PpuMI         406  AGGACCC              [GA]G^G[AT]CC[CT]
SmlI          352  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AfaI          567  GTAC                 GT^AC
              879  GTAC                
AvaI          391  CCCGAG               C^[CT]CG[GA]G
              400  CCCGAG              
BbeI          222  GGCGCC               GGCGC^C
              837  GGCGCC              
BglI           85  GCCGCCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              444  GCCGGCCAGGC         
BlpI          456  GCTGAGC              GC^T[ACGT]AGC
              606  GCTCAGC             
Bpu1102I      456  GCTGAGC              GC^T[ACGT]AGC
              606  GCTCAGC             
BseRI         349  CTCCTC               GAGGAG(10/8)
              121  GAGGAG              
BsgI          471  CTGCAC               GTGCAG(16/14)
              238  GTGCAG              
BsiEI         319  CGGCCG               CG[GA][CT]^CG
              773  CGGCCG              
BsmAI         619  GAGAC                GTCTC(1/5)
              666  GAGAC               
BsoBI         391  CCCGAG               C^[CT]CG[GA]G
              400  CCCGAG              
BssHII        585  GCGCGC               G^CGCGC
              819  GCGCGC              
CelII         456  GCTGAGC              GC^T[ACGT]AGC
              606  GCTCAGC             
DraII          74  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              406  AGGACCC             
EagI          319  CGGCCG               C^GGCCG
              773  CGGCCG              
EclXI         319  CGGCCG               C^GGCCG
              773  CGGCCG              
Eco52I        319  CGGCCG               C^GGCCG
              773  CGGCCG              
EcoO109I       74  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              406  AGGACCC             
HphI          627  GGTGA                GGTGA(8/7)
              518  TCACC               
KasI          222  GGCGCC               G^GCGCC
              837  GGCGCC              
NaeI           51  GCCGGC               GCC^GGC
              444  GCCGGC              
NarI          222  GGCGCC               GG^CGCC
              837  GGCGCC              
NgoMIV         51  GCCGGC               G^CCGGC
              444  GCCGGC              
NotI          318  GCGGCCGC             GC^GGCCGC
              772  GCGGCCGC            
PvuII         529  CAGCTG               CAG^CTG
              764  CAGCTG              
RsaI          567  GTAC                 GT^AC
              879  GTAC                
SfaNI         367  GATGC                GCATC(5/9)
              417  GATGC               
SfcI          767  CTGTAG               C^T[GA][CT]AG
              870  CTACAG              
SfoI          222  GGCGCC               GGC^GCC
              837  GGCGCC              
TspRI         266  CACTG                CA[GC]TG[ACGT][ACGT]^
              615  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwNI       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrFI       BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DsaI        EaeI        EcoRII      EcoT14I     
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       ItaI        KspI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIII      NlaIV       PleI        SacII       
Sau96I      ScrFI       StyI        TseI        
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