(Circular) (Six-base) MAPSORT of: pF1KB8940.seq Check: 1240 from: 1 to: 4004 pF1KB8940 4004 bp With 182 enzymes: SgfI * June 26, 2008 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 723 723 Size: 4004 AccI GT'mk_AC Cuts at: 1042 1042 Size: 4004 Acc65I G'GTAC_C Cuts at: 1020 1020 Size: 4004 AclI AA'CG_TT Cuts at: 3844 3844 Size: 4004 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 255 823 1829 2261 3008 3276 255 Size: 568 1006 432 747 268 983 Fragments arranged by size: 1006 983 747 568 432 268 AflIII A'CryG_T Cuts at: 1356 2460 3437 1356 Size: 1104 977 1923 Fragments arranged by size: 1923 1104 977 AgeI A'CCGG_T Cuts at: 2392 2392 Size: 4004 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 643 675 643 Size: 32 3972 AlwNI CAG_nnn'CTG Cuts at: 375 642 678 1309 2876 375 Size: 267 36 631 1567 1503 Fragments arranged by size: 1567 1503 631 267 36 ApaLI G'TGCA_C Cuts at: 2774 2774 Size: 4004 ApoI r'AATT_y Cuts at: 1008 3357 3613 1008 Size: 2349 256 1399 Fragments arranged by size: 2349 1399 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4004 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4004 AvaI C'yCGr_G Cuts at: 499 508 1024 499 Size: 9 516 3479 Fragments arranged by size: 3479 516 9 BamHI G'GATC_C Cuts at: 1029 1029 Size: 4004 BanI G'GyrC_C Cuts at: 330 892 907 945 1020 1434 1682 1717 330 Size: 562 15 38 75 414 248 35 2617 Fragments arranged by size: 2617 562 414 248 75 38 35 15 BanII G_rGCy'C Cuts at: 405 499 535 571 1018 2048 405 Size: 94 36 36 447 1030 2361 Fragments arranged by size: 2361 1030 447 94 36 36 BbeI G_GCGC'C Cuts at: 334 949 1438 1686 334 Size: 615 489 248 2652 Fragments arranged by size: 2652 615 489 248 BciVI GTATCCnnnnn_n' Cuts at: 1902 2663 4004 1902 Size: 761 1341 1902 Fragments arranged by size: 1902 1341 761 BclI T'GATC_A Cuts at: 1524 1524 Size: 4004 BglI GCCn_nnn'nGGC Cuts at: 199 558 199 Size: 359 3645 BglII A'GATC_T Cuts at: 1519 3556 1519 Size: 2037 1967 BlpI GC'TnA_GC Cuts at: 565 715 1116 565 Size: 150 401 3453 Fragments arranged by size: 3453 401 150 Bme1580I G_kGCm'C Cuts at: 1629 1722 2778 1629 Size: 93 1056 2855 Fragments arranged by size: 2855 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 385 1216 1297 1632 3776 385 Size: 831 81 335 2144 613 Fragments arranged by size: 2144 831 613 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2371 2371 Size: 4004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 670 690 814 670 Size: 20 124 3860 Fragments arranged by size: 3860 124 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 445 1180 2059 2551 2849 445 Size: 735 879 492 298 1600 Fragments arranged by size: 1600 879 735 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3708 3708 Size: 4004 BsaAI yAC'GTr Cuts at: 1357 1357 Size: 4004 BsaBI GATnn'nnATC Cuts at: 6 1518 3555 6 Size: 1512 2037 455 Fragments arranged by size: 2037 1512 455 BsaHI Gr'CG_yC Cuts at: 331 380 619 946 1435 1683 2385 3881 331 Size: 49 239 327 489 248 702 1496 454 Fragments arranged by size: 1496 702 489 454 327 248 239 49 BsaWI w'CCGG_w Cuts at: 1193 1714 2392 2666 2813 3224 1193 Size: 521 678 274 147 411 1973 Fragments arranged by size: 1973 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 643 673 643 Size: 30 3974 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 244 448 244 Size: 204 3800 BseYI C'CCAG_C Cuts at: 187 396 573 1429 2187 2764 3260 187 Size: 209 177 856 758 577 496 931 Fragments arranged by size: 931 856 758 577 496 209 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 367 564 367 Size: 197 3807 BsiEI CG_ry'CG Cuts at: 104 430 884 1546 1592 2800 104 Size: 326 454 662 46 1208 1308 Fragments arranged by size: 1308 1208 662 454 326 46 BsiHKAI G_wGCw'C Cuts at: 1018 1796 1986 2778 1018 Size: 778 190 792 2244 Fragments arranged by size: 2244 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 721 721 Size: 4004 Bsp1286I G_dGCh'C Cuts at: 405 499 535 571 1018 1629 1722 1796 1986 Size: 94 36 36 447 611 93 74 190 Cuts at: 1986 2048 2778 405 Size: 62 730 1631 Fragments arranged by size: 1631 730 611 447 190 94 93 74 62 36 36 BspEI T'CCGG_A Cuts at: 3224 3224 Size: 4004 BspHI T'CATG_A Cuts at: 3180 3180 Size: 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 723 1054 1268 1570 1951 723 Size: 331 214 302 381 2776 Fragments arranged by size: 2776 381 331 302 214 BsrBI CCG'CTC Cuts at: 50 432 2296 2350 3834 3999 50 Size: 382 1864 54 1484 165 55 Fragments arranged by size: 1864 1484 382 165 55 54 BsrDI GCAATG_nn' Cuts at: 1916 1916 Size: 4004 BsrFI r'CCGG_y Cuts at: 159 552 831 2002 2183 2392 159 Size: 393 279 1171 181 209 1771 Fragments arranged by size: 1771 1171 393 279 209 181 BssHII G'CGCG_C Cuts at: 693 927 693 Size: 234 3770 BssSI C'ACGA_G Cuts at: 2275 2633 2275 Size: 358 3646 BstAPI GCAn_nnn'nTGC Cuts at: 1381 1381 Size: 4004 BstBI TT'CG_AA Cuts at: 2365 2365 Size: 4004 BstYI r'GATC_y Cuts at: 1 1029 1496 1519 1854 2100 3112 3198 3210 Size: 1028 467 23 335 246 1012 86 12 Cuts at: 3210 3556 1 Size: 346 449 Fragments arranged by size: 1028 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 197 203 215 424 520 613 884 950 197 Size: 6 12 209 96 93 271 66 3251 Fragments arranged by size: 3251 271 209 96 93 66 12 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 669 1954 2135 2298 669 Size: 1285 181 163 2375 Fragments arranged by size: 2375 1285 181 163 BtsI GCAGTG_nn' Cuts at: 1247 1247 Size: 4004 DraI TTT'AAA Cuts at: 1003 1003 Size: 4004 DraIII CAC_nnn'GTG Cuts at: 127 127 Size: 4004 DrdI GACnn_nn'nnGTC Cuts at: 1710 2568 1710 Size: 858 3146 EaeI y'GGCC_r Cuts at: 200 290 427 435 554 809 881 1589 1763 Size: 90 137 8 119 255 72 708 174 Cuts at: 1763 2154 2181 3526 200 Size: 391 27 1345 678 Fragments arranged by size: 1345 708 678 391 255 174 137 119 90 72 27 8 EagI C'GGCC_G Cuts at: 427 881 1589 427 Size: 454 708 2842 Fragments arranged by size: 2842 708 454 EarI CTCTTCn'nnn_ Cuts at: 423 2027 2237 423 Size: 1604 210 2190 Fragments arranged by size: 2190 1604 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 135 652 2212 2522 2668 3814 135 Size: 517 1560 310 146 1146 325 Fragments arranged by size: 1560 1146 517 325 310 146 EcoICRI GAG'CTC Cuts at: 1016 1016 Size: 4004 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 255 670 690 814 823 1829 2261 3008 3276 Size: 415 20 124 9 1006 432 747 268 Cuts at: 3276 255 Size: 983 Fragments arranged by size: 1006 983 747 432 415 268 124 20 9 EcoO109I rG'GnC_Cy Cuts at: 183 515 1143 183 Size: 332 628 3044 Fragments arranged by size: 3044 628 332 EcoRI G'AATT_C Cuts at: 1008 1008 Size: 4004 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2023 2023 Size: 4004 FspI TGC'GCA Cuts at: 1785 1785 Size: 4004 HaeII r_GCGC'y Cuts at: 194 286 326 334 649 949 1438 1686 2708 Size: 92 40 8 315 300 489 248 1022 Cuts at: 2708 3687 194 Size: 979 511 Fragments arranged by size: 1022 979 511 489 315 300 248 92 40 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 400 432 1846 1876 1878 1908 400 Size: 32 1414 30 2 30 2496 Fragments arranged by size: 2496 1414 32 30 30 2 HincII GTy'rAC Cuts at: 1043 1043 Size: 4004 KasI G'GCGC_C Cuts at: 330 945 1434 1682 330 Size: 615 489 248 2652 Fragments arranged by size: 2652 615 489 248 KpnI G_GTAC'C Cuts at: 1024 1024 Size: 4004 MluI A'CGCG_T Cuts at: 3437 3437 Size: 4004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2675 2859 2675 Size: 184 3820 MscI TGG'CCA Cuts at: 1765 1765 Size: 4004 MslI CAynn'nnrTG Cuts at: 1993 2120 1993 Size: 127 3877 MspA1I CmG'CkG Cuts at: 199 426 615 639 874 886 1115 1429 1789 Size: 227 189 24 235 12 229 314 360 Cuts at: 1789 2802 3047 199 Size: 1013 245 1156 Fragments arranged by size: 1156 1013 360 314 245 235 229 227 189 24 12 NaeI GCC'GGC Cuts at: 161 554 2185 161 Size: 393 1631 1980 Fragments arranged by size: 1980 1631 393 NarI GG'CG_CC Cuts at: 331 946 1435 1683 331 Size: 615 489 248 2652 Fragments arranged by size: 2652 615 489 248 NcoI C'CATG_G Cuts at: 215 215 Size: 4004 NgoMIV G'CCGG_C Cuts at: 159 552 2183 159 Size: 393 1631 1980 Fragments arranged by size: 1980 1631 393 NotI GC'GGCC_GC Cuts at: 427 881 427 Size: 454 3550 NspI r_CATG'y Cuts at: 987 1057 1557 2464 987 Size: 70 500 907 2527 Fragments arranged by size: 2527 907 500 70 PciI A'CATG_T Cuts at: 2460 2460 Size: 4004 PmeI GTTT'AAAC Cuts at: 1003 1003 Size: 4004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 643 675 3143 3175 643 Size: 32 2468 32 1472 Fragments arranged by size: 2468 1472 32 32 PpuMI rG'GwC_Cy Cuts at: 515 515 Size: 4004 PstI C_TGCA'G Cuts at: 1051 1736 1051 Size: 685 3319 PvuI CG_AT'CG Cuts at: 104 104 Size: 4004 PvuII CAG'CTG Cuts at: 639 874 1429 1789 639 Size: 235 555 360 2854 Fragments arranged by size: 2854 555 360 235 RsrII CG'GwC_CG Cuts at: 2199 2199 Size: 4004 SacI G_AGCT'C Cuts at: 1018 1018 Size: 4004 SacII CC_GC'GG Cuts at: 200 427 616 887 200 Size: 227 189 271 3317 Fragments arranged by size: 3317 271 227 189 SalI G'TCGA_C Cuts at: 1041 1041 Size: 4004 SapI GCTCTTCn'nnn_ Cuts at: 2027 2237 2027 Size: 210 3794 SbfI CC_TGCA'GG Cuts at: 1051 1051 Size: 4004 ScaI AGT'ACT Cuts at: 3521 3521 Size: 4004 SfcI C'TryA_G Cuts at: 33 875 978 1047 1732 2725 2916 33 Size: 842 103 69 685 993 191 1121 Fragments arranged by size: 1121 993 842 685 191 103 69 SfoI GGC'GCC Cuts at: 332 947 1436 1684 332 Size: 615 489 248 2652 Fragments arranged by size: 2652 615 489 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4004 SmaI CCC'GGG Cuts at: 1026 1026 Size: 4004 SmlI C'TyrA_G Cuts at: 460 1159 2074 2566 2828 460 Size: 699 915 492 262 1636 Fragments arranged by size: 1636 915 699 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4004 SphI G_CATG'C Cuts at: 1057 1557 1057 Size: 500 3504 StyI C'CwwG_G Cuts at: 215 314 764 1138 1492 215 Size: 99 450 374 354 2727 Fragments arranged by size: 2727 450 374 354 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1587 1720 2179 2388 1587 Size: 133 459 209 3203 Fragments arranged by size: 3203 459 209 133 TatI w'GTAC_w Cuts at: 3519 3519 Size: 4004 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 365 397 3521 3553 365 Size: 32 3124 32 816 Fragments arranged by size: 3124 816 32 32 Tth111I GACn'n_nGTC Cuts at: 1801 1801 Size: 4004 XbaI T'CTAG_A Cuts at: 1035 1035 Size: 4004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1499 1499 Size: 4004 XmaI C'CCGG_G Cuts at: 1024 1024 Size: 4004 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI