Restriction map for
pF1KB8940
(Linear) (Six-base) MAPSORT of: KIBB8940.seq Check: 6026 from: 1 to: 891
KIBB8940.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 615 891
Size: 615 276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 715 891
Size: 147 568 176
Fragments arranged by size:
568 176 147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 535 567 891
Size: 535 32 324
Fragments arranged by size:
535 324 32
AlwNI CAG_nnn'CTG
Cuts at: 0 267 534 570 891
Size: 267 267 36 321
Fragments arranged by size:
321 267 267 36
AvaI C'yCGr_G
Cuts at: 0 391 400 891
Size: 391 9 491
Fragments arranged by size:
491 391 9
BanI G'GyrC_C
Cuts at: 0 222 784 799 837 891
Size: 222 562 15 38 54
Fragments arranged by size:
562 222 54 38 15
BanII G_rGCy'C
Cuts at: 0 297 391 427 463 891
Size: 297 94 36 36 428
Fragments arranged by size:
428 297 94 36 36
BbeI G_GCGC'C
Cuts at: 0 226 841 891
Size: 226 615 50
Fragments arranged by size:
615 226 50
BglI GCCn_nnn'nGGC
Cuts at: 0 91 450 891
Size: 91 359 441
Fragments arranged by size:
441 359 91
BlpI GC'TnA_GC
Cuts at: 0 457 607 891
Size: 457 150 284
Fragments arranged by size:
457 284 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 891
Size: 277 614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 582 706 891
Size: 562 20 124 185
Fragments arranged by size:
562 185 124 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 891
Size: 337 554
BsaHI Gr'CG_yC
Cuts at: 0 223 272 511 838 891
Size: 223 49 239 327 53
Fragments arranged by size:
327 239 223 53 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 535 565 891
Size: 535 30 326
Fragments arranged by size:
535 326 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 340 891
Size: 136 204 551
Fragments arranged by size:
551 204 136
BseYI C'CCAG_C
Cuts at: 0 79 288 465 891
Size: 79 209 177 426
Fragments arranged by size:
426 209 177 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 456 891
Size: 259 197 435
Fragments arranged by size:
435 259 197
BsiEI CG_ry'CG
Cuts at: 0 322 776 891
Size: 322 454 115
Fragments arranged by size:
454 322 115
BsmBI CGTCTCn'nnnn_
Cuts at: 0 613 891
Size: 613 278
Bsp1286I G_dGCh'C
Cuts at: 0 297 391 427 463 891
Size: 297 94 36 36 428
Fragments arranged by size:
428 297 94 36 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 615 891
Size: 615 276
BsrBI CCG'CTC
Cuts at: 0 324 891
Size: 324 567
BsrFI r'CCGG_y
Cuts at: 0 51 444 723 891
Size: 51 393 279 168
Fragments arranged by size:
393 279 168 51
BssHII G'CGCG_C
Cuts at: 0 585 819 891
Size: 585 234 72
Fragments arranged by size:
585 234 72
BtgI C'CryG_G
Cuts at: 0 89 95 107 316 412 505 776 842
Size: 89 6 12 209 96 93 271 66
Cuts at: 842 891
Size: 49
Fragments arranged by size:
271 209 96 93 89 66 49 12
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 561 891
Size: 561 330
DraIII CAC_nnn'GTG
Cuts at: 0 19 891
Size: 19 872
EaeI y'GGCC_r
Cuts at: 0 92 182 319 327 446 701 773 891
Size: 92 90 137 8 119 255 72 118
Fragments arranged by size:
255 137 119 118 92 90 72 8
EagI C'GGCC_G
Cuts at: 0 319 773 891
Size: 319 454 118
Fragments arranged by size:
454 319 118
EarI CTCTTCn'nnn_
Cuts at: 0 315 891
Size: 315 576
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 27 544 891
Size: 27 517 347
Fragments arranged by size:
517 347 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 562 582 706 715 891
Size: 147 415 20 124 9 176
Fragments arranged by size:
415 176 147 124 20 9
EcoO109I rG'GnC_Cy
Cuts at: 0 75 407 891
Size: 75 332 484
Fragments arranged by size:
484 332 75
HaeII r_GCGC'y
Cuts at: 0 86 178 218 226 541 841 891
Size: 86 92 40 8 315 300 50
Fragments arranged by size:
315 300 92 86 50 40 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 891
Size: 292 32 567
Fragments arranged by size:
567 292 32
KasI G'GCGC_C
Cuts at: 0 222 837 891
Size: 222 615 54
Fragments arranged by size:
615 222 54
MspA1I CmG'CkG
Cuts at: 0 91 318 507 531 766 778 891
Size: 91 227 189 24 235 12 113
Fragments arranged by size:
235 227 189 113 91 24 12
NaeI GCC'GGC
Cuts at: 0 53 446 891
Size: 53 393 445
Fragments arranged by size:
445 393 53
NarI GG'CG_CC
Cuts at: 0 223 838 891
Size: 223 615 53
Fragments arranged by size:
615 223 53
NcoI C'CATG_G
Cuts at: 0 107 891
Size: 107 784
NgoMIV G'CCGG_C
Cuts at: 0 51 444 891
Size: 51 393 447
Fragments arranged by size:
447 393 51
NotI GC'GGCC_GC
Cuts at: 0 319 773 891
Size: 319 454 118
Fragments arranged by size:
454 319 118
NspI r_CATG'y
Cuts at: 0 879 891
Size: 879 12
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 891
Size: 535 32 324
Fragments arranged by size:
535 324 32
PpuMI rG'GwC_Cy
Cuts at: 0 407 891
Size: 407 484
PvuII CAG'CTG
Cuts at: 0 531 766 891
Size: 531 235 125
Fragments arranged by size:
531 235 125
SacII CC_GC'GG
Cuts at: 0 92 319 508 779 891
Size: 92 227 189 271 112
Fragments arranged by size:
271 227 189 112 92
SfcI C'TryA_G
Cuts at: 0 767 870 891
Size: 767 103 21
Fragments arranged by size:
767 103 21
SfoI GGC'GCC
Cuts at: 0 224 839 891
Size: 224 615 52
Fragments arranged by size:
615 224 52
SmlI C'TyrA_G
Cuts at: 0 352 891
Size: 352 539
StyI C'CwwG_G
Cuts at: 0 107 206 656 891
Size: 107 99 450 235
Fragments arranged by size:
450 235 107 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 257 289 891
Size: 257 32 602
Fragments arranged by size:
602 257 32
Enzymes that do cut:
AarI AcuI AloI AlwNI AvaI BanI BanII BbeI
BglI BlpI BmrI BpmI BpuEI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssHII BtgI BtgZI DraIII EaeI EagI EarI EciI
Eco57MI EcoO109I HaeII Hin4I KasI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PpiI PpuMI PvuII SacII
SfcI SfoI SmlI StyI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII Bme1580I BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI