################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI StuI SwaI Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 142 GACGTC GACGT^C AcyI 142 GACGTC G[GA]^CG[CT]C AflIII 1225 ACACGT A^C[GA][CT]GT Alw44I 862 GTGCAC G^TGCAC ApaI 11 GGGCCC GGGCC^C ApaLI 862 GTGCAC G^TGCAC AspI 1012 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1251 GCTGAGG CCTCAGC(-5/-2) BglI 991 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1077 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1077 GCTGAGC GC^T[ACGT]AGC BsaAI 652 TACGTG [CT]AC^GT[GA] BsaHI 142 GACGTC G[GA]^CG[CT]C BsiEI 104 CGACCG CG[GA][CT]^CG BsiWI 154 CGTACG C^GTACG BspHI 1382 TCATGA T^CATGA BsrFI 49 GCCGGT [GA]^CCGG[CT] BssHII 62 GCGCGC G^CGCGC BstXI 1607 CCAGCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1077 GCTGAGC GC^T[ACGT]AGC Cfr10I 49 GCCGGT [GA]^CCGG[CT] DrdI 1191 GACCCCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 234 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1322 ATGCAT ATGCA^T HaeII 1598 GGCGCT [GA]GCGC^[CT] HgaI 165 GACGC GACGC(5/10) Hin1I 142 GACGTC G[GA]^CG[CT]C MseI 841 TTAA T^TAA NsiI 1322 ATGCAT ATGCA^T PflFI 1012 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 1382 TCATGA T^CATGA SacI 228 GAGCTC GAGCT^C SapI 625 GAAGAGC GCTCTTC(1/4) SbfI 1412 CCTGCAGG CCTGCA^GG SexAI 792 ACCTGGT A^CC[AT]GGT SmlI 1446 CTCAAG C^T[CT][GA]AG SphI 1270 GCATGC GCATG^C Sse8387I 1412 CCTGCAGG CCTGCA^GG SspI 755 AATATT AAT^ATT TaqI 1589 TCGA T^CGA Tru9I 841 TTAA T^TAA Tth111I 1012 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1589 TCGA T^CGA XcmI 1055 CCATGGTTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 687 GATCC GGATC(4/5) 210 GGATC AvaII 796 GGTCC G^G[AT]CC 1190 GGACC BalI 585 TGGCCA TGG^CCA 1568 TGGCCA BseRI 1424 CTCCTC GAGGAG(10/8) 1175 GAGGAG BssSI 859 CTCGTG CACGAG(-5/-1) 1702 CTCGTG BstYI 210 GGATCT [GA]^GATC[CT] 686 AGATCC DpnII 211 GATC ^GATC 687 GATC DraII 10 GGGGCCC [GA]G^G[ACGT]CC[CT] 782 GGGGCCT DsaI 1055 CCATGG C^C[GA][CT]GG 1186 CCATGG EcoO109I 10 GGGGCCC [GA]G^G[ACGT]CC[CT] 782 GGGGCCT MboI 211 GATC ^GATC 687 GATC MflI 210 GGATCT [GA]^GATC[CT] 686 AGATCC MluNI 585 TGGCCA TGG^CCA 1568 TGGCCA MscI 585 TGGCCA TGG^CCA 1568 TGGCCA NcoI 1055 CCATGG C^CATGG 1186 CCATGG NdeII 211 GATC ^GATC 687 GATC Sau3AI 211 GATC ^GATC 687 GATC SmaI 14 CCCGGG CCC^GGG 123 CCCGGG XhoII 210 GGATCT [GA]^GATC[CT] 686 AGATCC XmaCI 14 CCCGGG C^CCGGG 123 CCCGGG XmaI 14 CCCGGG C^CCGGG 123 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboII MnlI MslI MspI MvaI MvnI MwoI NciI NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I TspRI Van91I