(Circular) (Six-base) MAPSORT of: pF1KE0027.seq Check: 2410 from: 1 to: 4823 pF1KE0027 4823 bp With 182 enzymes: SgfI * March 2, 2010 14:30 .. AatII G_ACGT'C Cuts at: 254 254 Size: 4823 AccI GT'mk_AC Cuts at: 1861 1861 Size: 4823 Acc65I G'GTAC_C Cuts at: 1839 1839 Size: 4823 AclI AA'CG_TT Cuts at: 4663 4663 Size: 4823 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 343 2648 3080 3827 4095 343 Size: 2305 432 747 268 1071 Fragments arranged by size: 2305 1071 747 432 268 AflIII A'CryG_T Cuts at: 1333 2175 3279 4256 1333 Size: 842 1104 977 1900 Fragments arranged by size: 1900 1104 977 842 AgeI A'CCGG_T Cuts at: 3211 3211 Size: 4823 AleI CACnn'nnGTG Cuts at: 1094 1094 Size: 4823 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 212 995 1088 212 Size: 783 93 3947 Fragments arranged by size: 3947 783 93 AlwNI CAG_nnn'CTG Cuts at: 596 798 1129 1665 2128 3695 596 Size: 202 331 536 463 1567 1724 Fragments arranged by size: 1724 1567 536 463 331 202 ApaI G_GGCC'C Cuts at: 123 123 Size: 4823 ApaLI G'TGCA_C Cuts at: 970 3593 970 Size: 2623 2200 ApoI r'AATT_y Cuts at: 1827 4176 4432 1827 Size: 2349 256 2218 Fragments arranged by size: 2349 2218 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4823 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4823 AvaI C'yCGr_G Cuts at: 122 231 1036 1843 122 Size: 109 805 807 3102 Fragments arranged by size: 3102 807 805 109 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 709 742 709 Size: 33 4790 BamHI G'GATC_C Cuts at: 1848 1848 Size: 4823 BanI G'GyrC_C Cuts at: 1839 2253 2501 2536 1839 Size: 414 248 35 4126 Fragments arranged by size: 4126 414 248 35 BanII G_rGCy'C Cuts at: 123 230 340 362 1837 2867 123 Size: 107 110 22 1475 1030 2079 Fragments arranged by size: 2079 1475 1030 110 107 22 BbeI G_GCGC'C Cuts at: 2257 2505 2257 Size: 248 4575 BbvCI CC'TCA_GC Cuts at: 1360 1360 Size: 4823 BciVI GTATCCnnnnn_n' Cuts at: 2721 3482 4823 2721 Size: 761 1341 2721 Fragments arranged by size: 2721 1341 761 BclI T'GATC_A Cuts at: 2343 2343 Size: 4823 BfrBI ATG'CAT Cuts at: 1432 1432 Size: 4823 BglI GCCn_nnn'nGGC Cuts at: 1105 1105 Size: 4823 BglII A'GATC_T Cuts at: 2338 4375 2338 Size: 2037 2786 BlpI GC'TnA_GC Cuts at: 1186 1935 1186 Size: 749 4074 Bme1580I G_kGCm'C Cuts at: 123 247 974 1635 2448 2541 3597 123 Size: 124 727 661 813 93 1056 1349 Fragments arranged by size: 1349 1056 813 727 661 124 93 BmrI ACTGGGnnnn_n' Cuts at: 1341 1451 1615 1779 2035 2116 2451 4595 1341 Size: 110 164 164 256 81 335 2144 1569 Fragments arranged by size: 2144 1569 335 256 164 164 110 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 637 3190 637 Size: 2553 2270 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 519 703 760 1139 1229 1505 1751 519 Size: 184 57 379 90 276 246 3591 Fragments arranged by size: 3591 379 276 246 184 90 57 Bpu10I CC'TnA_GC Cuts at: 1360 1360 Size: 4823 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1539 1999 2878 3370 3668 1539 Size: 460 879 492 298 2694 Fragments arranged by size: 2694 879 492 460 298 BsaI GGTCTCn'nnnn_ Cuts at: 283 970 1676 4527 283 Size: 687 706 2851 579 Fragments arranged by size: 2851 706 687 579 BsaAI yAC'GTr Cuts at: 762 2176 762 Size: 1414 3409 BsaBI GATnn'nnATC Cuts at: 6 2337 4374 6 Size: 2331 2037 455 Fragments arranged by size: 2331 2037 455 BsaHI Gr'CG_yC Cuts at: 251 2254 2502 3204 4700 251 Size: 2003 248 702 1496 374 Fragments arranged by size: 2003 1496 702 374 248 BsaWI w'CCGG_w Cuts at: 2012 2533 3211 3485 3632 4043 2012 Size: 521 678 274 147 411 2792 Fragments arranged by size: 2792 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 624 654 1592 1622 624 Size: 30 938 30 3825 Fragments arranged by size: 3825 938 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1298 1523 1298 Size: 225 4598 BseYI C'CCAG_C Cuts at: 201 1709 2248 3006 3583 4079 201 Size: 1508 539 758 577 496 945 Fragments arranged by size: 1508 945 758 577 539 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 262 488 793 1639 262 Size: 226 305 846 3446 Fragments arranged by size: 3446 846 305 226 BsiEI CG_ry'CG Cuts at: 104 215 2365 2411 3619 104 Size: 111 2150 46 1208 1308 Fragments arranged by size: 2150 1308 1208 111 46 BsiHKAI G_wGCw'C Cuts at: 134 340 974 1742 1837 2615 2805 3597 134 Size: 206 634 768 95 778 190 792 1360 Fragments arranged by size: 1360 792 778 768 634 206 190 95 BsiWI C'GTAC_G Cuts at: 262 262 Size: 4823 Bsp1286I G_dGCh'C Cuts at: 123 134 230 247 340 362 974 1635 1742 Size: 11 96 17 93 22 612 661 107 Cuts at: 1742 1837 2448 2541 2615 2805 2867 3597 123 Size: 95 611 93 74 190 62 730 1349 Fragments arranged by size: 1349 730 661 612 611 190 107 96 95 93 93 74 62 22 17 11 BspEI T'CCGG_A Cuts at: 4043 4043 Size: 4823 BspHI T'CATG_A Cuts at: 1490 3999 1490 Size: 2509 2314 BspMI ACCTGCnnnn'nnnn_ Cuts at: 664 815 1070 1873 2087 2389 2770 664 Size: 151 255 803 214 302 381 2717 Fragments arranged by size: 2717 803 381 302 255 214 151 BsrBI CCG'CTC Cuts at: 50 3115 3169 4653 4818 50 Size: 3065 54 1484 165 55 Fragments arranged by size: 3065 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2735 2735 Size: 4823 BsrFI r'CCGG_y Cuts at: 157 2821 3002 3211 157 Size: 2664 181 209 1769 Fragments arranged by size: 2664 1769 209 181 BssHII G'CGCG_C Cuts at: 170 170 Size: 4823 BssSI C'ACGA_G Cuts at: 967 1810 3094 3452 967 Size: 843 1284 358 2338 Fragments arranged by size: 2338 1284 843 358 BstAPI GCAn_nnn'nTGC Cuts at: 2200 2200 Size: 4823 BstBI TT'CG_AA Cuts at: 3184 3184 Size: 4823 BstXI CCAn_nnnn'nTGG Cuts at: 1722 1722 Size: 4823 BstYI r'GATC_y Cuts at: 1 318 794 1848 2315 2338 2673 2919 3931 Size: 317 476 1054 467 23 335 246 1012 Cuts at: 3931 4017 4029 4375 1 Size: 86 12 346 449 Fragments arranged by size: 1054 1012 476 467 449 346 335 317 246 86 23 12 BtgI C'CryG_G Cuts at: 107 1163 1294 107 Size: 1056 131 3636 Fragments arranged by size: 3636 1056 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2773 2954 3117 2773 Size: 181 163 4479 Fragments arranged by size: 4479 181 163 BtsI GCAGTG_nn' Cuts at: 136 1089 1101 1613 2066 136 Size: 953 12 512 453 2893 Fragments arranged by size: 2893 953 512 453 12 DraI TTT'AAA Cuts at: 1822 1822 Size: 4823 DrdI GACnn_nn'nnGTC Cuts at: 1305 2529 3387 1305 Size: 1224 858 2741 Fragments arranged by size: 2741 1224 858 EaeI y'GGCC_r Cuts at: 282 693 1676 2408 2582 2973 3000 4345 282 Size: 411 983 732 174 391 27 1345 760 Fragments arranged by size: 1345 983 760 732 411 391 174 27 EagI C'GGCC_G Cuts at: 2408 2408 Size: 4823 EarI CTCTTCn'nnn_ Cuts at: 728 1555 1650 2846 3056 728 Size: 827 95 1196 210 2495 Fragments arranged by size: 2495 1196 827 210 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3031 3341 3487 4633 3031 Size: 310 146 1146 3221 Fragments arranged by size: 3221 1146 310 146 EcoICRI GAG'CTC Cuts at: 338 1835 338 Size: 1497 3326 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 343 519 703 760 1139 1229 1505 1751 2648 Size: 176 184 57 379 90 276 246 897 Cuts at: 2648 3080 3827 4095 343 Size: 432 747 268 1071 Fragments arranged by size: 1071 897 747 432 379 276 268 246 184 176 90 57 EcoNI CCTnn'n_nnAGG Cuts at: 346 346 Size: 4823 EcoO109I rG'GnC_Cy Cuts at: 119 891 1962 119 Size: 772 1071 2980 Fragments arranged by size: 2980 1071 772 EcoRI G'AATT_C Cuts at: 1827 1827 Size: 4823 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2842 2842 Size: 4823 FspI TGC'GCA Cuts at: 2604 2604 Size: 4823 HaeII r_GCGC'y Cuts at: 1710 2257 2505 3527 4506 1710 Size: 547 248 1022 979 2027 Fragments arranged by size: 2027 1022 979 547 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 296 328 805 837 1211 1243 2665 2695 2697 Size: 32 477 32 374 32 1422 30 2 Cuts at: 2697 2727 296 Size: 30 2392 Fragments arranged by size: 2392 1422 477 374 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1862 1862 Size: 4823 KasI G'GCGC_C Cuts at: 2253 2501 2253 Size: 248 4575 KpnI G_GTAC'C Cuts at: 1843 1843 Size: 4823 MluI A'CGCG_T Cuts at: 4256 4256 Size: 4823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 359 3494 3678 359 Size: 3135 184 1504 Fragments arranged by size: 3135 1504 184 MscI TGG'CCA Cuts at: 695 1678 2584 695 Size: 983 906 2934 Fragments arranged by size: 2934 983 906 MslI CAynn'nnrTG Cuts at: 313 640 1070 1094 1621 2812 2939 313 Size: 327 430 24 527 1191 127 2197 Fragments arranged by size: 2197 1191 527 430 327 127 24 MspA1I CmG'CkG Cuts at: 1934 2248 2608 3621 3866 1934 Size: 314 360 1013 245 2891 Fragments arranged by size: 2891 1013 360 314 245 NaeI GCC'GGC Cuts at: 3004 3004 Size: 4823 NarI GG'CG_CC Cuts at: 2254 2502 2254 Size: 248 4575 NcoI C'CATG_G Cuts at: 107 1163 1294 107 Size: 1056 131 3636 Fragments arranged by size: 3636 1056 131 NgoMIV G'CCGG_C Cuts at: 3002 3002 Size: 4823 NsiI A_TGCA'T Cuts at: 1434 1434 Size: 4823 NspI r_CATG'y Cuts at: 1185 1316 1382 1436 1876 2376 3283 1185 Size: 131 66 54 440 500 907 2725 Fragments arranged by size: 2725 907 500 440 131 66 54 PasI CC'CwG_GG Cuts at: 1402 1402 Size: 4823 PciI A'CATG_T Cuts at: 3279 3279 Size: 4823 PflMI CCAn_nnn'nTGG Cuts at: 1295 1353 1627 1665 1295 Size: 58 274 38 4453 Fragments arranged by size: 4453 274 58 38 PmeI GTTT'AAAC Cuts at: 1822 1822 Size: 4823 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 3962 Size: 32 4791 PspOMI G'GGCC_C Cuts at: 119 119 Size: 4823 PstI C_TGCA'G Cuts at: 143 1096 1525 1564 1766 1870 2555 143 Size: 953 429 39 202 104 685 2411 Fragments arranged by size: 2411 953 685 429 202 104 39 PvuI CG_AT'CG Cuts at: 104 104 Size: 4823 PvuII CAG'CTG Cuts at: 2248 2608 2248 Size: 360 4463 RsrII CG'GwC_CG Cuts at: 3018 3018 Size: 4823 SacI G_AGCT'C Cuts at: 340 1837 340 Size: 1497 3326 SalI G'TCGA_C Cuts at: 1860 1860 Size: 4823 SapI GCTCTTCn'nnn_ Cuts at: 728 2846 3056 728 Size: 2118 210 2495 Fragments arranged by size: 2495 2118 210 SbfI CC_TGCA'GG Cuts at: 1525 1870 1525 Size: 345 4478 ScaI AGT'ACT Cuts at: 4340 4340 Size: 4823 SexAI A'CCwGG_T Cuts at: 900 900 Size: 4823 SfcI C'TryA_G Cuts at: 33 139 363 575 1092 1109 1521 1560 1762 Size: 106 224 212 517 17 412 39 202 Cuts at: 1762 1866 2551 3544 3735 33 Size: 104 685 993 191 1121 Fragments arranged by size: 1121 993 685 517 412 224 212 202 191 106 104 39 17 SfoI GGC'GCC Cuts at: 2255 2503 2255 Size: 248 4575 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4823 SmaI CCC'GGG Cuts at: 124 233 1845 124 Size: 109 1612 3102 Fragments arranged by size: 3102 1612 109 SmlI C'TyrA_G Cuts at: 1554 1978 2893 3385 3647 1554 Size: 424 915 492 262 2730 Fragments arranged by size: 2730 915 492 424 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4823 SphI G_CATG'C Cuts at: 1382 1876 2376 1382 Size: 494 500 3829 Fragments arranged by size: 3829 500 494 SspI AAT'ATT Cuts at: 865 865 Size: 4823 StyI C'CwwG_G Cuts at: 107 221 696 1010 1163 1238 1294 1595 1673 Size: 114 475 314 153 75 56 301 78 Cuts at: 1673 1957 2311 107 Size: 284 354 2619 Fragments arranged by size: 2619 475 354 314 301 284 153 114 78 75 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 229 1003 2406 2539 2998 3207 229 Size: 774 1403 133 459 209 1845 Fragments arranged by size: 1845 1403 774 459 209 133 TatI w'GTAC_w Cuts at: 653 4338 653 Size: 3685 1138 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1592 1624 4340 4372 1592 Size: 32 2716 32 2043 Fragments arranged by size: 2716 2043 32 32 Tth111I GACn'n_nGTC Cuts at: 1123 2620 1123 Size: 1497 3326 XbaI T'CTAG_A Cuts at: 1854 1854 Size: 4823 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1170 2318 1170 Size: 1148 3675 XmaI C'CCGG_G Cuts at: 122 231 1843 122 Size: 109 1612 3102 Fragments arranged by size: 3102 1612 109 ZraI GAC'GTC Cuts at: 252 252 Size: 4823 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AhdI AloI AscI AvrII BbsI BcgI BmgBI BmtI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI