Restriction map for pF1KB8234
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HindIII     HpaI        KasI        
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NarI        NciI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        596  GGTACC               G^GTACC
AcyI            6  GGCGTC               G[GA]^CG[CT]C
AflIII        277  ACATGT               A^C[GA][CT]GT
AlwI          474  GGATC                GGATC(4/5)
Asp718I       596  GGTACC               G^GTACC
AspHI         767  GTGCTC               G[AT]GC[AT]^C
AvaII         519  GGACC                G^G[AT]CC
BanI          596  GGTACC               G^G[CT][GA]CC
BanII         160  GAGCCC               G[GA]GC[CT]^C
BbsI          273  GAAGAC               GAAGAC(2/6)
BbvCI          27  GCTGAGG              CCTCAGC(-5/-2)
BbvI          435  GCAGC                GCAGC(8/12)
BfaI          719  CTAG                 C^TAG
BpmI           85  CTCCAG               CTGGAG(16/14)
BpuAI         273  GAAGAC               GAAGAC(2/6)
BsaHI           6  GGCGTC               G[GA]^CG[CT]C
BsaWI         624  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       767  GTGCTC               G[AT]GC[AT]^C
BsmFI         518  GGGAC                GGGAC(10/14)
BspLU11I      277  ACATGT               A^CATGT
BsrFI         650  GCCGGC               [GA]^CCGG[CT]
BstEII        586  GGTGACC              G^GT[ACGT]ACC
BstPI         586  GGTGACC              G^GT[ACGT]ACC
BstXI         240  CCACAAGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          383  GCGC                 GCG^C
Cfr10I        650  GCCGGC               [GA]^CCGG[CT]
EcoO65I       586  GGTGACC              G^GT[ACGT]ACC
EcoT14I       601  CCATGG               C^C[AT][AT]GG
HgaI            7  GCGTC                GACGC(5/10)
HhaI          383  GCGC                 GCG^C
Hin1I           6  GGCGTC               G[GA]^CG[CT]C
HinP1I        383  GCGC                 G^CGC
HphI          586  GGTGA                GGTGA(8/7)
KpnI          596  GGTACC               GGTAC^C
MaeI          719  CTAG                 C^TAG
MaeII         356  ACGT                 A^CGT
MaeIII        587  GTGAC                ^GT[ACGT]AC
MseI           16  TTAA                 T^TAA
MslI          241  CACAAGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          650  GCCGGC               GCC^GGC
NcoI          601  CCATGG               C^CATGG
NgoMIV        650  GCCGGC               G^CCGGC
PpuMI         518  GGGACCT              [GA]G^G[AT]CC[CT]
SfaNI         711  GATGC                GCATC(5/9)
StyI          601  CCATGG               C^C[AT][AT]GG
TaqI          324  TCGA                 T^CGA
Tru9I          16  TTAA                 T^TAA
TseI          435  GCAGC                G^C[AT]GC
Tsp45I        587  GTGAC                ^GT[GC]AC
Tsp509I       374  AATT                 ^AATT
TspRI         744  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       324  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AluI          105  AGCT                 AG^CT
              362  AGCT                
BmyI          160  GAGCCC               G[AGT]GC[ACT]^C
              767  GTGCTC              
BsaI          481  GGTCTC               GGTCTC(1/5)
              579  GGTCTC              
BseRI         484  CTCCTC               GAGGAG(10/8)
              738  CTCCTC              
Bsp1286I      160  GAGCCC               G[AGT]GC[ACT]^C
              767  GTGCTC              
BsrI          611  ACTGG                ACTGG(1/-1)
              745  ACTGG               
Cac8I         564  GCCAGC               GC[ACGT]^[ACGT]GC
              650  GCCGGC              
DpnII         328  GATC                 ^GATC
              475  GATC                
DraII         518  GGGACCT              [GA]G^G[ACGT]CC[CT]
              756  AGGCCCT             
DsaI          514  CCACGG               C^C[GA][CT]GG
              601  CCATGG              
EarI           52  CTCTTC               CTCTTC(1/4)
              676  GAAGAG              
EcoO109I      518  GGGACCT              [GA]G^G[ACGT]CC[CT]
              756  AGGCCCT             
HapII         625  CCGG                 C^CGG
              651  CCGG                
HincII        343  GTTGAC               GT[CT]^[GA]AC
              447  GTTGAC              
HindII        343  GTTGAC               GT[CT]^[GA]AC
              447  GTTGAC              
HpaII         625  CCGG                 C^CGG
              651  CCGG                
Ksp632I        52  CTCTTC               CTCTTC(1/4)
              676  GAAGAG              
MboI          328  GATC                 ^GATC
              475  GATC                
MspA1I        262  CAGCGG               C[AC]G^C[GT]G
              728  CAGCGG              
MspI          625  CCGG                 C^CGG
              651  CCGG                
NdeII         328  GATC                 ^GATC
              475  GATC                
NspI          277  ACATGT               [GA]CATG^[CT]
              339  GCATGT              
Sau3AI        328  GATC                 ^GATC
              475  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        AfaI        BsaJI       BsiYI       BslI        
BsmAI       BssKI       BstF5I      BstNI       Cfr13I      
DdeI        EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MboII       MnlI        MvaI        
MwoI        NlaIII      NlaIV       PleI        RsaI        
Sau96I      ScrFI       TfiI        XcmI        
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