(Circular) (Six-base) MAPSORT of: pF1KE0139.seq Check: 5843 from: 1 to: 3653 pF1KE0139 3653 bp With 182 enzymes: SgfI * April 24, 2009 13:11 .. AccI GT'mk_AC Cuts at: 542 691 542 Size: 149 3504 Acc65I G'GTAC_C Cuts at: 669 669 Size: 3653 AclI AA'CG_TT Cuts at: 3493 3493 Size: 3653 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 230 1478 1910 2657 2925 230 Size: 1248 432 747 268 958 Fragments arranged by size: 1248 958 747 432 268 AflIII A'CryG_T Cuts at: 1005 2109 3086 1005 Size: 1104 977 1572 Fragments arranged by size: 1572 1104 977 AgeI A'CCGG_T Cuts at: 2041 2041 Size: 3653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 528 528 Size: 3653 AlwNI CAG_nnn'CTG Cuts at: 958 2525 958 Size: 1567 2086 ApaLI G'TGCA_C Cuts at: 2423 2423 Size: 3653 ApoI r'AATT_y Cuts at: 247 657 3006 3262 247 Size: 410 2349 256 638 Fragments arranged by size: 2349 638 410 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3653 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3653 AvaI C'yCGr_G Cuts at: 673 673 Size: 3653 BamHI G'GATC_C Cuts at: 678 678 Size: 3653 BanI G'GyrC_C Cuts at: 669 1083 1331 1366 669 Size: 414 248 35 2956 Fragments arranged by size: 2956 414 248 35 BanII G_rGCy'C Cuts at: 667 1697 667 Size: 1030 2623 BbeI G_GCGC'C Cuts at: 1087 1335 1087 Size: 248 3405 BciVI GTATCCnnnnn_n' Cuts at: 1551 2312 3653 1551 Size: 761 1341 1551 Fragments arranged by size: 1551 1341 761 BclI T'GATC_A Cuts at: 1173 1173 Size: 3653 BglII A'GATC_T Cuts at: 1168 3205 1168 Size: 2037 1616 BlpI GC'TnA_GC Cuts at: 765 765 Size: 3653 Bme1580I G_kGCm'C Cuts at: 1278 1371 2427 1278 Size: 93 1056 2504 Fragments arranged by size: 2504 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 865 946 1281 3425 865 Size: 81 335 2144 1093 Fragments arranged by size: 2144 1093 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 585 2020 585 Size: 1435 2218 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 295 295 Size: 3653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 829 1708 2200 2498 829 Size: 879 492 298 1984 Fragments arranged by size: 1984 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3357 3357 Size: 3653 BsaAI yAC'GTr Cuts at: 1006 1006 Size: 3653 BsaBI GATnn'nnATC Cuts at: 6 1167 3204 6 Size: 1161 2037 455 Fragments arranged by size: 2037 1161 455 BsaHI Gr'CG_yC Cuts at: 1084 1332 2034 3530 1084 Size: 248 702 1496 1207 Fragments arranged by size: 1496 1207 702 248 BsaWI w'CCGG_w Cuts at: 257 842 1363 2041 2315 2462 2873 257 Size: 585 521 678 274 147 411 1037 Fragments arranged by size: 1037 678 585 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 268 298 268 Size: 30 3623 BseYI C'CCAG_C Cuts at: 1078 1836 2413 2909 1078 Size: 758 577 496 1822 Fragments arranged by size: 1822 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 183 183 Size: 3653 BsiEI CG_ry'CG Cuts at: 104 121 1195 1241 2449 104 Size: 17 1074 46 1208 1308 Fragments arranged by size: 1308 1208 1074 46 17 BsiHKAI G_wGCw'C Cuts at: 667 1445 1635 2427 667 Size: 778 190 792 1893 Fragments arranged by size: 1893 792 778 190 Bsp1286I G_dGCh'C Cuts at: 667 1278 1371 1445 1635 1697 2427 667 Size: 611 93 74 190 62 730 1893 Fragments arranged by size: 1893 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2873 2873 Size: 3653 BspHI T'CATG_A Cuts at: 2829 2829 Size: 3653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 155 703 917 1219 1600 155 Size: 548 214 302 381 2208 Fragments arranged by size: 2208 548 381 302 214 BsrBI CCG'CTC Cuts at: 50 303 1945 1999 3483 3648 50 Size: 253 1642 54 1484 165 55 Fragments arranged by size: 1642 1484 253 165 55 54 BsrDI GCAATG_nn' Cuts at: 1565 1565 Size: 3653 BsrFI r'CCGG_y Cuts at: 195 1651 1832 2041 195 Size: 1456 181 209 1807 Fragments arranged by size: 1807 1456 209 181 BssSI C'ACGA_G Cuts at: 428 1924 2282 428 Size: 1496 358 1799 Fragments arranged by size: 1799 1496 358 BstAPI GCAn_nnn'nTGC Cuts at: 348 1030 348 Size: 682 2971 BstBI TT'CG_AA Cuts at: 2014 2014 Size: 3653 BstXI CCAn_nnnn'nTGG Cuts at: 471 557 471 Size: 86 3567 BstYI r'GATC_y Cuts at: 1 678 1145 1168 1503 1749 2761 2847 2859 Size: 677 467 23 335 246 1012 86 12 Cuts at: 2859 3205 1 Size: 346 449 Fragments arranged by size: 1012 677 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 556 107 Size: 449 3204 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1603 1784 1947 1603 Size: 181 163 3309 Fragments arranged by size: 3309 181 163 BtsI GCAGTG_nn' Cuts at: 896 896 Size: 3653 DraI TTT'AAA Cuts at: 652 652 Size: 3653 DrdI GACnn_nn'nnGTC Cuts at: 1359 2217 1359 Size: 858 2795 EaeI y'GGCC_r Cuts at: 1238 1412 1803 1830 3175 1238 Size: 174 391 27 1345 1716 Fragments arranged by size: 1716 1345 391 174 27 EagI C'GGCC_G Cuts at: 1238 1238 Size: 3653 EarI CTCTTCn'nnn_ Cuts at: 1676 1886 1676 Size: 210 3443 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 327 1861 2171 2317 3463 327 Size: 1534 310 146 1146 517 Fragments arranged by size: 1534 1146 517 310 146 EcoICRI GAG'CTC Cuts at: 665 665 Size: 3653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 230 295 1478 1910 2657 2925 230 Size: 65 1183 432 747 268 958 Fragments arranged by size: 1183 958 747 432 268 65 EcoO109I rG'GnC_Cy Cuts at: 792 792 Size: 3653 EcoRI G'AATT_C Cuts at: 247 657 247 Size: 410 3243 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1672 1672 Size: 3653 FspI TGC'GCA Cuts at: 1434 1434 Size: 3653 HaeII r_GCGC'y Cuts at: 115 1087 1335 2357 3336 115 Size: 972 248 1022 979 432 Fragments arranged by size: 1022 979 972 432 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 268 300 354 386 1495 1525 1527 1557 268 Size: 32 54 32 1109 30 2 30 2364 Fragments arranged by size: 2364 1109 54 32 32 30 30 2 HincII GTy'rAC Cuts at: 182 692 182 Size: 510 3143 KasI G'GCGC_C Cuts at: 1083 1331 1083 Size: 248 3405 KpnI G_GTAC'C Cuts at: 673 673 Size: 3653 MluI A'CGCG_T Cuts at: 3086 3086 Size: 3653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2324 2508 2324 Size: 184 3469 MscI TGG'CCA Cuts at: 1414 1414 Size: 3653 MslI CAynn'nnrTG Cuts at: 398 510 1642 1769 398 Size: 112 1132 127 2282 Fragments arranged by size: 2282 1132 127 112 MspA1I CmG'CkG Cuts at: 764 1078 1438 2451 2696 764 Size: 314 360 1013 245 1721 Fragments arranged by size: 1721 1013 360 314 245 NaeI GCC'GGC Cuts at: 197 1834 197 Size: 1637 2016 NarI GG'CG_CC Cuts at: 1084 1332 1084 Size: 248 3405 NcoI C'CATG_G Cuts at: 107 107 Size: 3653 NgoMIV G'CCGG_C Cuts at: 195 1832 195 Size: 1637 2016 NspI r_CATG'y Cuts at: 706 1206 2113 706 Size: 500 907 2246 Fragments arranged by size: 2246 907 500 PasI CC'CwG_GG Cuts at: 157 157 Size: 3653 PciI A'CATG_T Cuts at: 2109 2109 Size: 3653 PmeI GTTT'AAAC Cuts at: 652 652 Size: 3653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2792 2824 2792 Size: 32 3621 PsiI TTA'TAA Cuts at: 358 358 Size: 3653 PstI C_TGCA'G Cuts at: 700 1385 700 Size: 685 2968 PvuI CG_AT'CG Cuts at: 104 104 Size: 3653 PvuII CAG'CTG Cuts at: 1078 1438 1078 Size: 360 3293 RsrII CG'GwC_CG Cuts at: 1848 1848 Size: 3653 SacI G_AGCT'C Cuts at: 667 667 Size: 3653 SalI G'TCGA_C Cuts at: 690 690 Size: 3653 SapI GCTCTTCn'nnn_ Cuts at: 1676 1886 1676 Size: 210 3443 SbfI CC_TGCA'GG Cuts at: 700 700 Size: 3653 ScaI AGT'ACT Cuts at: 3170 3170 Size: 3653 SfcI C'TryA_G Cuts at: 33 287 696 1381 2374 2565 33 Size: 254 409 685 993 191 1121 Fragments arranged by size: 1121 993 685 409 254 191 SfoI GGC'GCC Cuts at: 1085 1333 1085 Size: 248 3405 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3653 SmaI CCC'GGG Cuts at: 675 675 Size: 3653 SmlI C'TyrA_G Cuts at: 808 1723 2215 2477 808 Size: 915 492 262 1984 Fragments arranged by size: 1984 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3653 SphI G_CATG'C Cuts at: 706 1206 706 Size: 500 3153 StyI C'CwwG_G Cuts at: 107 787 1141 107 Size: 680 354 2619 Fragments arranged by size: 2619 680 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 107 149 1236 1369 1828 2037 107 Size: 42 1087 133 459 209 1723 Fragments arranged by size: 1723 1087 459 209 133 42 TatI w'GTAC_w Cuts at: 3168 3168 Size: 3653 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3170 3202 3170 Size: 32 3621 Tth111I GACn'n_nGTC Cuts at: 1450 1450 Size: 3653 XbaI T'CTAG_A Cuts at: 684 684 Size: 3653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 471 1148 471 Size: 677 2976 XmaI C'CCGG_G Cuts at: 673 673 Size: 3653 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI