Restriction map for pF1KE0552
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AviII       AvrII       BamHI       BbeI        BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaHI       BsaI        BseRI       
BsiEI       BsiHKAI     Bsp1407I    BspDI       BspHI       
BspMI       BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MfeI        MluI        
MseI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccI          643  GTAGAC               GT^[AC][GT]AC
AccIII        539  TCCGGA               T^CCGGA
AflIII        765  ACATGT               A^C[GA][CT]GT
AlwNI         481  CAGTCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       312  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          685  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          774  TGGCCA               TGG^CCA
BanII         295  GGGCTC               G[GA]GC[CT]^C
BbrPI          19  CACGTG               CAC^GTG
BmyI          295  GGGCTC               G[AGT]GC[ACT]^C
BsaAI          19  CACGTG               [CT]AC^GT[GA]
BsaBI         357  GATCGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         539  TCCGGA               [AT]^CCGG[AT]
BseAI         539  TCCGGA               T^CCGGA
BsiWI         251  CGTACG               C^GTACG
BsmAI         211  GTCTC                GTCTC(1/5)
BsmBI         210  CGTCTC               CGTCTC(1/5)
Bsp1286I      295  GGGCTC               G[AGT]GC[ACT]^C
BspEI         539  TCCGGA               T^CCGGA
BspLU11I      765  ACATGT               A^CATGT
BsrBI          29  CCGCTC               CCGCTC(-3/-3)
BsrFI          50  GCCGGC               [GA]^CCGG[CT]
BssSI         376  CACGAG               CACGAG(-5/-1)
BstAPI        480  GCAGTCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        522  GGTCACC              G^GT[ACGT]ACC
BstPI         522  GGTCACC              G^GT[ACGT]ACC
BstYI         542  GGATCT               [GA]^GATC[CT]
CfoI          747  GCGC                 GCG^C
Cfr10I         50  GCCGGC               [GA]^CCGG[CT]
DdeI          287  CTGAG                C^T[ACGT]AG
DraII         689  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI          514  GACTCGGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      689  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       522  GGTCACC              G^GT[ACGT]ACC
HhaI          747  GCGC                 GCG^C
HinP1I        747  GCGC                 G^CGC
HinfI         514  GACTC                G^A[ACGT]TC
KspI          757  CCGCGG               CCGC^GG
MamI          357  GATCGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          542  GGATCT               [GA]^GATC[CT]
MluNI         774  TGGCCA               TGG^CCA
MroI          539  TCCGGA               T^CCGGA
MscI          774  TGGCCA               TGG^CCA
NaeI           50  GCCGGC               GCC^GGC
NgoMIV         50  GCCGGC               G^CCGGC
PaeR7I        225  CTCGAG               C^TCGAG
PflFI         685  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          18  CCACGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          514  GACTC                GAGTC(4/5)
PmaCI          19  CACGTG               CAC^GTG
PmlI           19  CACGTG               CAC^GTG
PpuMI         689  GGGTCCC              [GA]G^G[AT]CC[CT]
SacII         757  CCGCGG               CCGC^GG
SmlI          225  CTCGAG               C^T[CT][GA]AG
StuI           95  AGGCCT               AGG^CCT
Tsp45I        523  GTCAC                ^GT[GC]AC
Tth111I       685  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         18  CCACGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          225  CTCGAG               C^TCGAG
XhoII         542  GGATCT               [GA]^GATC[CT]
XmnI          312  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwI          267  GATCC                GGATC(4/5)
              542  GGATC               
AvaI          225  CTCGAG               C^[CT]CG[GA]G
              300  CCCGAG              
BanI           38  GGCACC               G^G[CT][GA]CC
              162  GGTGCC              
BcnI          159  CCCGG                CC^[GC]GG
              896  CCCGG               
BpmI          185  CTCCAG               CTGGAG(16/14)
              613  CTGGAG              
BsmI          828  GAATGC               GAATGC(1/-1)
                4  GCATTC              
BsoBI         225  CTCGAG               C^[CT]CG[GA]G
              300  CCCGAG              
EcoT14I       556  CCATGG               C^C[AT][AT]GG
              660  CCATGG              
MaeII          20  ACGT                 A^CGT
              260  ACGT                
MaeIII        523  GTCAC                ^GT[ACGT]AC
              762  GTTAC               
MboII         312  GAAGA                GAAGA(8/7)
              699  GAAGA               
MspA1I        706  CAGCGG               C[AC]G^C[GT]G
              757  CCGCGG              
NciI          159  CCCGG                CC^[GC]GG
              896  CCCGG               
NcoI          556  CCATGG               C^CATGG
              660  CCATGG              
NspI          141  GCATGT               [GA]CATG^[CT]
              765  ACATGT              
StyI          556  CCATGG               C^C[AT][AT]GG
              660  CCATGG              
XcmI          556  CCATGGCAGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              783  CCACGAAGGGTATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaII       
BbvI        BsaJI       BsgI        BsiYI       BslI        
BsmFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       Cfr13I      DpnII       DsaI        
EaeI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HpaII       HphI        
ItaI        MboI        MnlI        MslI        MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        TspRI       TthHB8I     
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