(Circular) (Six-base) MAPSORT of: pF1KE0552.seq Check: 2586 from: 1 to: 4019 pF1KE0552 4019 bp With 182 enzymes: SgfI * April 24, 2009 13:15 .. AccI GT'mk_AC Cuts at: 752 1057 752 Size: 305 3714 Acc65I G'GTAC_C Cuts at: 1035 1035 Size: 4019 AclI AA'CG_TT Cuts at: 3859 3859 Size: 4019 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 583 601 1844 2276 3023 3291 583 Size: 18 1243 432 747 268 1311 Fragments arranged by size: 1311 1243 747 432 268 18 AflIII A'CryG_T Cuts at: 873 1371 2475 3452 873 Size: 498 1104 977 1440 Fragments arranged by size: 1440 1104 977 498 AgeI A'CCGG_T Cuts at: 2407 2407 Size: 4019 AleI CACnn'nnGTG Cuts at: 931 931 Size: 4019 AlwNI CAG_nnn'CTG Cuts at: 594 1324 2891 594 Size: 730 1567 1722 Fragments arranged by size: 1722 1567 730 ApaLI G'TGCA_C Cuts at: 2789 2789 Size: 4019 ApoI r'AATT_y Cuts at: 1023 3372 3628 1023 Size: 2349 256 1414 Fragments arranged by size: 2349 1414 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4019 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4019 AvaI C'yCGr_G Cuts at: 333 408 1039 333 Size: 75 631 3313 Fragments arranged by size: 3313 631 75 BamHI G'GATC_C Cuts at: 1044 1044 Size: 4019 BanI G'GyrC_C Cuts at: 146 270 1035 1449 1697 1732 146 Size: 124 765 414 248 35 2433 Fragments arranged by size: 2433 765 414 248 124 35 BanII G_rGCy'C Cuts at: 407 1033 2063 407 Size: 626 1030 2363 Fragments arranged by size: 2363 1030 626 BbeI G_GCGC'C Cuts at: 1453 1701 1453 Size: 248 3771 BciVI GTATCCnnnnn_n' Cuts at: 1917 2678 4019 1917 Size: 761 1341 1917 Fragments arranged by size: 1917 1341 761 BclI T'GATC_A Cuts at: 1539 1539 Size: 4019 BglII A'GATC_T Cuts at: 1534 3571 1534 Size: 2037 1982 BlpI GC'TnA_GC Cuts at: 1131 1131 Size: 4019 Bme1580I G_kGCm'C Cuts at: 1644 1737 2793 1644 Size: 93 1056 2870 Fragments arranged by size: 2870 1056 93 BmgBI CAC'GTC Cuts at: 369 369 Size: 4019 BmrI ACTGGGnnnn_n' Cuts at: 114 790 804 1231 1312 1647 3791 114 Size: 676 14 427 81 335 2144 342 Fragments arranged by size: 2144 676 427 342 335 81 14 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 204 2386 204 Size: 2182 1837 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 278 742 278 Size: 464 3555 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1195 2074 2566 2864 1195 Size: 879 492 298 2350 Fragments arranged by size: 2350 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3723 3723 Size: 4019 BsaAI yAC'GTr Cuts at: 129 1372 129 Size: 1243 2776 BsaBI GATnn'nnATC Cuts at: 6 469 1533 3570 6 Size: 463 1064 2037 455 Fragments arranged by size: 2037 1064 463 455 BsaHI Gr'CG_yC Cuts at: 1450 1698 2400 3896 1450 Size: 248 702 1496 1573 Fragments arranged by size: 1573 1496 702 248 BsaWI w'CCGG_w Cuts at: 647 1208 1729 2407 2681 2828 3239 647 Size: 561 521 678 274 147 411 1427 Fragments arranged by size: 1427 678 561 521 411 274 147 BseYI C'CCAG_C Cuts at: 382 399 438 686 1444 2202 2779 3275 382 Size: 17 39 248 758 758 577 496 1126 Fragments arranged by size: 1126 758 758 577 496 248 39 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 274 607 868 274 Size: 333 261 3425 Fragments arranged by size: 3425 333 261 BsiEI CG_ry'CG Cuts at: 104 1561 1607 2815 104 Size: 1457 46 1208 1308 Fragments arranged by size: 1457 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1033 1811 2001 2793 1033 Size: 778 190 792 2259 Fragments arranged by size: 2259 792 778 190 BsiWI C'GTAC_G Cuts at: 359 359 Size: 4019 BsmI GAATG_Cn' Cuts at: 112 942 112 Size: 830 3189 BsmBI CGTCTCn'nnnn_ Cuts at: 324 324 Size: 4019 Bsp1286I G_dGCh'C Cuts at: 407 1033 1644 1737 1811 2001 2063 2793 407 Size: 626 611 93 74 190 62 730 1633 Fragments arranged by size: 1633 730 626 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 647 3239 647 Size: 2592 1427 BspHI T'CATG_A Cuts at: 3195 3195 Size: 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1069 1283 1585 1966 1069 Size: 214 302 381 3122 Fragments arranged by size: 3122 381 302 214 BsrBI CCG'CTC Cuts at: 50 139 2311 2365 3849 4014 50 Size: 89 2172 54 1484 165 55 Fragments arranged by size: 2172 1484 165 89 55 54 BsrDI GCAATG_nn' Cuts at: 1931 1931 Size: 4019 BsrFI r'CCGG_y Cuts at: 158 2017 2198 2407 158 Size: 1859 181 209 1770 Fragments arranged by size: 1859 1770 209 181 BssSI C'ACGA_G Cuts at: 484 2290 2648 484 Size: 1806 358 1855 Fragments arranged by size: 1855 1806 358 BstAPI GCAn_nnn'nTGC Cuts at: 594 1396 594 Size: 802 3217 BstBI TT'CG_AA Cuts at: 2380 2380 Size: 4019 BstEII G'GTnAC_C Cuts at: 630 630 Size: 4019 BstYI r'GATC_y Cuts at: 1 650 1044 1511 1534 1869 2115 3127 3213 Size: 649 394 467 23 335 246 1012 86 Cuts at: 3213 3225 3571 1 Size: 12 346 449 Fragments arranged by size: 1012 649 467 449 394 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 664 768 865 107 Size: 557 104 97 3261 Fragments arranged by size: 3261 557 104 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1969 2150 2313 1969 Size: 181 163 3675 Fragments arranged by size: 3675 181 163 BtsI GCAGTG_nn' Cuts at: 1262 1262 Size: 4019 DraI TTT'AAA Cuts at: 1018 1018 Size: 4019 DrdI GACnn_nn'nnGTC Cuts at: 628 1725 2583 628 Size: 1097 858 2064 Fragments arranged by size: 2064 1097 858 EaeI y'GGCC_r Cuts at: 134 512 882 1604 1778 2169 2196 3541 134 Size: 378 370 722 174 391 27 1345 612 Fragments arranged by size: 1345 722 612 391 378 370 174 27 EagI C'GGCC_G Cuts at: 1604 1604 Size: 4019 EarI CTCTTCn'nnn_ Cuts at: 2042 2252 2042 Size: 210 3809 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2227 2537 2683 3829 2227 Size: 310 146 1146 2417 Fragments arranged by size: 2417 1146 310 146 EcoICRI GAG'CTC Cuts at: 1031 1031 Size: 4019 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 278 583 601 742 1844 2276 3023 3291 278 Size: 305 18 141 1102 432 747 268 1006 Fragments arranged by size: 1102 1006 747 432 305 268 141 18 EcoO109I rG'GnC_Cy Cuts at: 798 1158 798 Size: 360 3659 EcoRI G'AATT_C Cuts at: 1023 1023 Size: 4019 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 549 2038 549 Size: 1489 2530 FspI TGC'GCA Cuts at: 1800 1800 Size: 4019 HaeII r_GCGC'y Cuts at: 1453 1701 2723 3702 1453 Size: 248 1022 979 1770 Fragments arranged by size: 1770 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 123 155 316 348 1861 1891 1893 1923 123 Size: 32 161 32 1513 30 2 30 2219 Fragments arranged by size: 2219 1513 161 32 32 30 30 2 HincII GTy'rAC Cuts at: 1058 1058 Size: 4019 KasI G'GCGC_C Cuts at: 1449 1697 1449 Size: 248 3771 KpnI G_GTAC'C Cuts at: 1039 1039 Size: 4019 MluI A'CGCG_T Cuts at: 3452 3452 Size: 4019 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 215 2690 2874 215 Size: 2475 184 1360 Fragments arranged by size: 2475 1360 184 MscI TGG'CCA Cuts at: 884 1780 884 Size: 896 3123 MslI CAynn'nnrTG Cuts at: 172 352 931 2008 2135 172 Size: 180 579 1077 127 2056 Fragments arranged by size: 2056 1077 579 180 127 MspA1I CmG'CkG Cuts at: 816 867 1130 1444 1804 2817 3062 816 Size: 51 263 314 360 1013 245 1773 Fragments arranged by size: 1773 1013 360 314 263 245 51 NaeI GCC'GGC Cuts at: 160 2200 160 Size: 2040 1979 NarI GG'CG_CC Cuts at: 1450 1698 1450 Size: 248 3771 NcoI C'CATG_G Cuts at: 107 664 768 107 Size: 557 104 3358 Fragments arranged by size: 3358 557 104 NgoMIV G'CCGG_C Cuts at: 158 2198 158 Size: 2040 1979 NspI r_CATG'y Cuts at: 253 877 1072 1572 2479 253 Size: 624 195 500 907 1793 Fragments arranged by size: 1793 907 624 500 195 PciI A'CATG_T Cuts at: 873 2475 873 Size: 1602 2417 PflMI CCAn_nnn'nTGG Cuts at: 132 132 Size: 4019 PfoI T'CCnGG_A Cuts at: 788 788 Size: 4019 PmeI GTTT'AAAC Cuts at: 1018 1018 Size: 4019 PmlI CAC'GTG Cuts at: 129 129 Size: 4019 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3158 3190 3158 Size: 32 3987 PpuMI rG'GwC_Cy Cuts at: 798 798 Size: 4019 PspXI vC'TCGA_Gb Cuts at: 333 333 Size: 4019 PstI C_TGCA'G Cuts at: 1066 1751 1066 Size: 685 3334 PvuI CG_AT'CG Cuts at: 104 104 Size: 4019 PvuII CAG'CTG Cuts at: 1444 1804 1444 Size: 360 3659 RsrII CG'GwC_CG Cuts at: 2214 2214 Size: 4019 SacI G_AGCT'C Cuts at: 1033 1033 Size: 4019 SacII CC_GC'GG Cuts at: 868 868 Size: 4019 SalI G'TCGA_C Cuts at: 1056 1056 Size: 4019 SanDI GG'GwC_CC Cuts at: 798 798 Size: 4019 SapI GCTCTTCn'nnn_ Cuts at: 2042 2252 2042 Size: 210 3809 SbfI CC_TGCA'GG Cuts at: 1066 1066 Size: 4019 ScaI AGT'ACT Cuts at: 3536 3536 Size: 4019 SfcI C'TryA_G Cuts at: 33 1062 1747 2740 2931 33 Size: 1029 685 993 191 1121 Fragments arranged by size: 1121 1029 993 685 191 SfoI GGC'GCC Cuts at: 1451 1699 1451 Size: 248 3771 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4019 SmaI CCC'GGG Cuts at: 1041 1041 Size: 4019 SmlI C'TyrA_G Cuts at: 333 1174 2089 2581 2843 333 Size: 841 915 492 262 1509 Fragments arranged by size: 1509 915 841 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4019 SphI G_CATG'C Cuts at: 1072 1572 1072 Size: 500 3519 StuI AGG'CCT Cuts at: 205 205 Size: 4019 StyI C'CwwG_G Cuts at: 107 664 768 1153 1507 107 Size: 557 104 385 354 2619 Fragments arranged by size: 2619 557 385 354 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 214 1602 1735 2194 2403 214 Size: 1388 133 459 209 1830 Fragments arranged by size: 1830 1388 459 209 133 TatI w'GTAC_w Cuts at: 182 458 3534 182 Size: 276 3076 667 Fragments arranged by size: 3076 667 276 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 770 802 3536 3568 770 Size: 32 2734 32 1221 Fragments arranged by size: 2734 1221 32 32 Tth111I GACn'n_nGTC Cuts at: 796 1816 796 Size: 1020 2999 XbaI T'CTAG_A Cuts at: 1050 1050 Size: 4019 XcmI CCAnnnn_n'nnnnTGG Cuts at: 671 898 1514 671 Size: 227 616 3176 Fragments arranged by size: 3176 616 227 XhoI C'TCGA_G Cuts at: 333 333 Size: 4019 XmaI C'CCGG_G Cuts at: 1039 1039 Size: 4019 XmnI GAAnn'nnTTC Cuts at: 424 424 Size: 4019 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI BseRI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PshAI PsiI PspOMI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI