Restriction map for pF1KB8217
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        Alw44I      AlwI        AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BpuAI       BsaBI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspLU11I    
BsrBI       BsrDI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        DsaI        EagI        
EarI        EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HindIII     HinfI       HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MseI        MunI        NarI        NcoI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacII       SapI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          847  GTCGAC               GT^[AC][GT]AC
AccIII        584  TCCGGA               T^CCGGA
AcyI          772  GGCGTC               G[GA]^CG[CT]C
AflIII        455  ACGTGT               A^C[GA][CT]GT
AhdI          558  GACCATGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       161  AGCGCT               AGC^GCT
AspEI         558  GACCATGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          659  TGGCCA               TGG^CCA
BglI          218  GCCCAAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          859  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      859  GCTGAGC              GC^T[ACGT]AGC
BsaAI         454  TACGTG               [CT]AC^GT[GA]
BsaHI         772  GGCGTC               G[GA]^CG[CT]C
BsaWI         584  TCCGGA               [AT]^CCGG[AT]
BseAI         584  TCCGGA               T^CCGGA
Bsp1407I      458  TGTACA               T^GTACA
BspEI         584  TCCGGA               T^CCGGA
BspHI         434  TCATGA               T^CATGA
BspMI         105  ACCTGC               ACCTGC(4/8)
BsrGI         458  TGTACA               T^GTACA
BssHII          7  GCGCGC               G^CGCGC
CelII         859  GCTGAGC              GC^T[ACGT]AGC
DdeI          860  CTGAG                C^T[ACGT]AG
Eam1105I      558  GACCATGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      161  AGCGCT               AGC^GCT
EcoNI         690  CCTGGCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         161  AGCGCT               [GA]GCGC^[CT]
Hin1I         772  GGCGTC               G[GA]^CG[CT]C
HincII        847  GTCGAC               GT[CT]^[GA]AC
HindII        847  GTCGAC               GT[CT]^[GA]AC
MaeII         455  ACGT                 A^CGT
MboII         254  TCTTC                GAAGA(8/7)
MluNI         659  TGGCCA               TGG^CCA
MroI          584  TCCGGA               T^CCGGA
MscI          659  TGGCCA               TGG^CCA
MslI          429  CATGTTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         12  CAGCGG               C[AC]G^C[GT]G
PflMI         651  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          434  TCATGA               T^CATGA
SacI          622  GAGCTC               GAGCT^C
SalI          847  GTCGAC               G^TCGAC
SspBI         458  TGTACA               T^GTACA
Van91I        651  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          390  CCACTCAAGGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AlwNI         652  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              828  CAGCCCCTG           
AspHI         530  GAGCAC               G[AT]GC[AT]^C
              622  GAGCTC              
AvaI          281  CCCGGG               C^[CT]CG[GA]G
              764  CCCGGG              
BfaI           62  CTAG                 C^TAG
              304  CTAG                
BsaI          352  GAGACC               GGTCTC(1/5)
              556  GAGACC              
BsiHKAI       530  GAGCAC               G[AT]GC[AT]^C
              622  GAGCTC              
BsmAI         352  GAGAC                GTCTC(1/5)
              556  GAGAC               
BsoBI         281  CCCGGG               C^[CT]CG[GA]G
              764  CCCGGG              
MaeI           62  CTAG                 C^TAG
              304  CTAG                
NspI          428  GCATGT               [GA]CATG^[CT]
              473  GCATGT              
PstI          107  CTGCAG               CTGCA^G
              627  CTGCAG              
SmaI          281  CCCGGG               CCC^GGG
              764  CCCGGG              
TaqI          257  TCGA                 T^CGA
              848  TCGA                
TthHB8I       257  TCGA                 T^CGA
              848  TCGA                
XmaCI         281  CCCGGG               C^CCGGG
              764  CCCGGG              
XmaI          281  CCCGGG               C^CCGGG
              764  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        ApaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DraII       EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HpaII       
ItaI        MnlI        MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NgoMIV      NlaIII      
NlaIV       PpuMI       RsaI        Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TseI        TspRI       
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