Restriction map for pF1KB8217
 (Linear) (Six-base) MAPSORT of: KIBB8217.seq  Check: 6323  from: 1  to: 879



KIBB8217.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     114     879 
   Size:        114     765
 
AccI GT'mk_AC

Cuts at:      0     848     879 
   Size:        848      31
 
AfeI AGC'GCT

Cuts at:      0     163     879 
   Size:        163     716
 
AflIII A'CryG_T

Cuts at:      0     455     879 
   Size:        455     424
 
AhdI GACnn_n'nnGTC

Cuts at:      0     563     879 
   Size:        563     316
 
AlwNI CAG_nnn'CTG

Cuts at:      0     657     833     879 
   Size:        657     176      46

  Fragments arranged by size: 

                 657     176      46
 
ApaI G_GGCC'C

Cuts at:      0      21     193     798     879 
   Size:         21     172     605      81

  Fragments arranged by size: 

                 605     172      81      21
 
AvaI C'yCGr_G

Cuts at:      0     281     764     879 
   Size:        281     483     115

  Fragments arranged by size: 

                 483     281     115
 
BanI G'GyrC_C

Cuts at:      0     102     144     592     879 
   Size:        102      42     448     287

  Fragments arranged by size: 

                 448     287     102      42
 
BanII G_rGCy'C

Cuts at:      0      21     193     542     626     737     798     879 
   Size:         21     172     349      84     111      61      81

  Fragments arranged by size: 

                 349     172     111      84      81      61      21
 
BglI GCCn_nnn'nGGC

Cuts at:      0     224     879 
   Size:        224     655
 
BlpI GC'TnA_GC

Cuts at:      0     860     879 
   Size:        860      19
 
Bme1580I G_kGCm'C

Cuts at:      0      21     193     798     879 
   Size:         21     172     605      81

  Fragments arranged by size: 

                 605     172      81      21
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      15     250     731     879 
   Size:         15     235     481     148

  Fragments arranged by size: 

                 481     235     148      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480     556     574     736     751     879 
   Size:        480      76      18     162      15     128

  Fragments arranged by size: 

                 480     162     128      76      18      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     378     595     879 
   Size:        198     180     217     284

  Fragments arranged by size: 

                 284     217     198     180
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     346     550     879 
   Size:        346     204     329

  Fragments arranged by size: 

                 346     329     204
 
BsaAI yAC'GTr

Cuts at:      0     456     879 
   Size:        456     423
 
BsaHI Gr'CG_yC

Cuts at:      0     773     879 
   Size:        773     106
 
BsaWI w'CCGG_w

Cuts at:      0     584     879 
   Size:        584     295
 
BsiHKAI G_wGCw'C

Cuts at:      0     534     626     879 
   Size:        534      92     253

  Fragments arranged by size: 

                 534     253      92
 
Bsp1286I G_dGCh'C

Cuts at:      0      21     193     534     542     626     737     798     879 
   Size:         21     172     341       8      84     111      61      81

  Fragments arranged by size: 

                 341     172     111      84      81      61      21       8
            
 
BspEI T'CCGG_A

Cuts at:      0     584     879 
   Size:        584     295
 
BspHI T'CATG_A

Cuts at:      0     434     879 
   Size:        434     445
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     114     879 
   Size:        114     765
 
BsrFI r'CCGG_y

Cuts at:      0     264     404     744     879 
   Size:        264     140     340     135

  Fragments arranged by size: 

                 340     264     140     135
 
BsrGI T'GTAC_A

Cuts at:      0     458     879 
   Size:        458     421
 
BssHII G'CGCG_C

Cuts at:      0       7     879 
   Size:          7     872
 
BtsI GCAGTG_nn'

Cuts at:      0     636     879 
   Size:        636     243
 
EaeI y'GGCC_r

Cuts at:      0     402     659     777     879 
   Size:        402     257     118     102

  Fragments arranged by size: 

                 402     257     118     102
 
EcoICRI GAG'CTC

Cuts at:      0     624     879 
   Size:        624     255
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     480     556     574     736     751     879 
   Size:        480      76      18     162      15     128

  Fragments arranged by size: 

                 480     162     128      76      18      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     694     879 
   Size:        694     185
 
EcoO109I rG'GnC_Cy

Cuts at:      0      17     300     446     654     687     879 
   Size:         17     283     146     208      33     192

  Fragments arranged by size: 

                 283     208     192     146      33      17
 
HaeII r_GCGC'y

Cuts at:      0     165     879 
   Size:        165     714
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     329     361     549     581     879 
   Size:        329      32     188      32     298

  Fragments arranged by size: 

                 329     298     188      32      32
 
HincII GTy'rAC

Cuts at:      0     849     879 
   Size:        849      30
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     227     879 
   Size:        113     114     652

  Fragments arranged by size: 

                 652     114     113
 
MscI TGG'CCA

Cuts at:      0     661     879 
   Size:        661     218
 
MslI CAynn'nnrTG

Cuts at:      0     433     879 
   Size:        433     446
 
MspA1I CmG'CkG

Cuts at:      0      14     879 
   Size:         14     865
 
NaeI GCC'GGC

Cuts at:      0     266     406     746     879 
   Size:        266     140     340     133

  Fragments arranged by size: 

                 340     266     140     133
 
NgoMIV G'CCGG_C

Cuts at:      0     264     404     744     879 
   Size:        264     140     340     135

  Fragments arranged by size: 

                 340     264     140     135
 
NspI r_CATG'y

Cuts at:      0     432     477     879 
   Size:        432      45     402

  Fragments arranged by size: 

                 432     402      45
 
PasI CC'CwG_GG

Cuts at:      0     155     833     879 
   Size:        155     678      46

  Fragments arranged by size: 

                 678     155      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     657     879 
   Size:        657     222
 
PpuMI rG'GwC_Cy

Cuts at:      0     300     446     687     879 
   Size:        300     146     241     192

  Fragments arranged by size: 

                 300     241     192     146
 
PspOMI G'GGCC_C

Cuts at:      0      17     189     794     879 
   Size:         17     172     605      85

  Fragments arranged by size: 

                 605     172      85      17
 
PstI C_TGCA'G

Cuts at:      0     111     631     879 
   Size:        111     520     248

  Fragments arranged by size: 

                 520     248     111
 
SacI G_AGCT'C

Cuts at:      0     626     879 
   Size:        626     253
 
SalI G'TCGA_C

Cuts at:      0     847     879 
   Size:        847      32
 
SanDI GG'GwC_CC

Cuts at:      0     446     879 
   Size:        446     433
 
SfcI C'TryA_G

Cuts at:      0     107     627     749     879 
   Size:        107     520     122     130

  Fragments arranged by size: 

                 520     130     122     107
 
SmaI CCC'GGG

Cuts at:      0     283     766     879 
   Size:        283     483     113

  Fragments arranged by size: 

                 483     283     113
 
SmlI C'TyrA_G

Cuts at:      0     213     393     610     879 
   Size:        213     180     217     269

  Fragments arranged by size: 

                 269     217     213     180
 
SrfI GCCC'GGGC

Cuts at:      0     283     879 
   Size:        283     596
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     370     879 
   Size:        370     509
 
TatI w'GTAC_w

Cuts at:      0     458     476     879 
   Size:        458      18     403

  Fragments arranged by size: 

                 458     403      18
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      70     102     112     144     879 
   Size:         70      32      10      32     735

  Fragments arranged by size: 

                 735      70      32      32      10
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     397     879 
   Size:        397     482
 
XmaI C'CCGG_G

Cuts at:      0     281     764     879 
   Size:        281     483     115

  Fragments arranged by size: 

                 483     281     115
 

 Enzymes that do cut:

     AarI     AccI     AfeI   AflIII     AhdI    AlwNI     ApaI     AvaI
     BanI    BanII     BglI     BlpI Bme1580I     BmrI     BpmI    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrFI    BsrGI   BssHII     BtsI     EaeI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I   HincII     MmeI     MscI     MslI
   MspA1I     NaeI   NgoMIV     NspI     PasI    PflMI    PpuMI   PspOMI
     PstI     SacI     SalI    SanDI     SfcI     SmaI     SmlI     SrfI
    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AleI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI     BplI   Bpu10I    BsaBI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BsrBI    BsrDI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     StuI
     StyI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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