Restriction map for pF1KB7952
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspI        AviII       AvrII       BalI        BbeI        
BbrPI       BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstPI       
BstUI       BstZ17I     Cac8I       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DsaI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT22I     
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        HpaII       KasI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        926  GGTACC               G^GTACC
AciI          967  GCGG                 CCGC(-3/-1)
AcsI          821  GAATTC               [GA]^AATT[CT]
AfaI          927  GTAC                 GT^AC
AhdI          440  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1166  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          508  CCTAAGG              CC^T[ACGT]AGG
ApoI          821  GAATTC               [GA]^AATT[CT]
Asp718I       926  GGTACC               G^GTACC
AspEI         440  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI           46  CCCGAG               C^[CT]CG[GA]G
AvaII         193  GGACC                G^G[AT]CC
BamHI         681  GGATCC               G^GATCC
BbsI          952  GTCTTC               GAAGAC(2/6)
BbvI         1019  GCAGC                GCAGC(8/12)
BcgI          166  CGACAGCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          472  CTAG                 C^TAG
BlpI         1171  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1171  GCTGAGC              GC^T[ACGT]AGC
BpuAI         952  GTCTTC               GAAGAC(2/6)
BsmFI         501  GTCCC                GGGAC(10/14)
BsoBI          46  CCCGAG               C^[CT]CG[GA]G
BstAPI       1019  GCAGCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         549  CCATTCTATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        508  CCTAAGG              CC^T[ACGT]AGG
CelII        1171  GCTGAGC              GC^T[ACGT]AGC
DraIII       1162  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          492  GACCCTTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      440  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        508  CCTAAGG              CC^T[ACGT]AGG
EcoRI         821  GAATTC               G^AATTC
FauI          967  GCGGG                CCCGC(4/6)
Fnu4HI       1019  GCAGC                GC^[ACGT]GC
HindIII      1119  AAGCTT               A^AGCTT
ItaI         1019  GCAGC                GC^[ACGT]GC
KpnI          926  GGTACC               GGTAC^C
MaeI          472  CTAG                 C^TAG
MslI          760  CACAAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        169  CAGCTG               C[AC]G^C[GT]G
PvuII         169  CAGCTG               CAG^CTG
RsaI          927  GTAC                 GT^AC
SapI          780  GAAGAGC              GCTCTTC(1/4)
SfaNI         981  GCATC                GCATC(5/9)
SfcI         1038  CTACAG               C^T[GA][CT]AG
TseI         1019  GCAGC                G^C[AT]GC
Tsp509I       822  AATT                 ^AATT
XcmI          180  CCAAGAGACCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflIII        249  ACATGT               A^C[GA][CT]GT
              526  ACATGT              
BanI          465  GGTGCC               G^G[CT][GA]CC
              926  GGTACC              
BsaI          184  GAGACC               GGTCTC(1/5)
              802  GGTCTC              
BsgI         1017  GTGCAG               GTGCAG(16/14)
             1026  GTGCAG              
BspLU11I      249  ACATGT               A^CATGT
              526  ACATGT              
Cfr13I        193  GGACC                G^G[ACGT]CC
              426  GGCCC               
EarI          361  GAAGAG               CTCTTC(1/4)
              780  GAAGAG              
EcoT14I       196  CCAAGG               C^C[AT][AT]GG
              314  CCAAGG              
HaeIII        245  GGCC                 GG^CC
              426  GGCC                
Ksp632I       361  GAAGAG               CTCTTC(1/4)
              780  GAAGAG              
NspI          249  ACATGT               [GA]CATG^[CT]
              526  ACATGT              
PflMI         349  CCAGAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1161  CCACACAGTGG         
PleI          215  GACTC                GAGTC(4/5)
              532  GACTC               
Sau96I        193  GGACC                G^G[ACGT]CC
              426  GGCCC               
StyI          196  CCAAGG               C^C[AT][AT]GG
              314  CCAAGG              
Tsp45I         92  GTCAC                ^GT[GC]AC
              530  GTGAC               
Van91I        349  CCAGAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1161  CCACACAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AluI        AlwI        Asp700I     AspHI       BanII       
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstNI       BstYI       DdeI        DpnII       EcoRII      
HinfI       HphI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       SacI        Sau3AI      
ScrFI       TaqI        TfiI        Tru9I       TspRI       
TthHB8I     XhoII       XmnI        
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