Restriction map for pF1KA0136
 (Linear) (Six-base) MAPSORT of: KIAA0136.seq  Check: 3213  from: 1  to: 2817



KIAA0136.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237    1376    1946    2042    2588    2719    2817 
   Size:        237    1139     570      96     546     131      98

  Fragments arranged by size: 

                1139     570     546     237     131      98      96
 
AflII C'TTAA_G

Cuts at:      0    1054    2817 
   Size:       1054    1763
 
AflIII A'CryG_T

Cuts at:      0    2373    2817 
   Size:       2373     444
 
AlwNI CAG_nnn'CTG

Cuts at:      0     100    2817 
   Size:        100    2717
 
ApoI r'AATT_y

Cuts at:      0      63     659     877    1185    1417    1551    1692    2817 
   Size:         63     596     218     308     232     134     141    1125

  Fragments arranged by size: 

                1125     596     308     232     218     141     134      63
            
 
AseI AT'TA_AT

Cuts at:      0     106     470    1454    2276    2817 
   Size:        106     364     984     822     541

  Fragments arranged by size: 

                 984     822     541     364     106
 
AvaI C'yCGr_G

Cuts at:      0     664    2817 
   Size:        664    2153
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1913    1946    2817 
   Size:       1913      33     871

  Fragments arranged by size: 

                1913     871      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     826    1536    1973    2817 
   Size:        826     710     437     844

  Fragments arranged by size: 

                 844     826     710     437
 
BbvCI CC'TCA_GC

Cuts at:      0      31    2817 
   Size:         31    2786
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2044    2078    2817 
   Size:       2044      34     739

  Fragments arranged by size: 

                2044     739      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      17    2183    2817 
   Size:         17    2166     634

  Fragments arranged by size: 

                2166     634      17
 
BclI T'GATC_A

Cuts at:      0    1218    2163    2817 
   Size:       1218     945     654

  Fragments arranged by size: 

                1218     945     654
 
BlpI GC'TnA_GC

Cuts at:      0     241    2817 
   Size:        241    2576
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     280    2817 
   Size:        280    2537
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2683    2817 
   Size:       2683     134
 
Bpu10I CC'TnA_GC

Cuts at:      0      31    2817 
   Size:         31    2786
 
BsaAI yAC'GTr

Cuts at:      0     805    2817 
   Size:        805    2012
 
BsaBI GATnn'nnATC

Cuts at:      0     866    2729    2817 
   Size:        866    1863      88

  Fragments arranged by size: 

                1863     866      88
 
BsaWI w'CCGG_w

Cuts at:      0    1434    2817 
   Size:       1434    1383
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1662    1692    2817 
   Size:       1662      30    1125

  Fragments arranged by size: 

                1662    1125      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2650    2817 
   Size:       2650     167
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113    2461    2817 
   Size:        113    2348     356

  Fragments arranged by size: 

                2348     356     113
 
BsmI GAATG_Cn'

Cuts at:      0     445     794    1662    2392    2817 
   Size:        445     349     868     730     425

  Fragments arranged by size: 

                 868     730     445     425     349
 
BspHI T'CATG_A

Cuts at:      0    1773    2817 
   Size:       1773    1044
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1770    2817 
   Size:       1770    1047
 
BssHII G'CGCG_C

Cuts at:      0      35    2817 
   Size:         35    2782
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2071    2817 
   Size:       2071     746
 
BstBI TT'CG_AA

Cuts at:      0     835    2817 
   Size:        835    1982
 
Bsu36I CC'TnA_GG

Cuts at:      0     751    1511    2817 
   Size:        751     760    1306

  Fragments arranged by size: 

                1306     760     751
 
BtgI C'CryG_G

Cuts at:      0      18     527    2817 
   Size:         18     509    2290

  Fragments arranged by size: 

                2290     509      18
 
BtsI GCAGTG_nn'

Cuts at:      0    2000    2817 
   Size:       2000     817
 
DraIII CAC_nnn'GTG

Cuts at:      0    2551    2817 
   Size:       2551     266
 
EaeI y'GGCC_r

Cuts at:      0      82    1474    1857    2817 
   Size:         82    1392     383     960

  Fragments arranged by size: 

                1392     960     383      82
 
EarI CTCTTCn'nnn_

Cuts at:      0     832    2687    2817 
   Size:        832    1855     130

  Fragments arranged by size: 

                1855     832     130
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1272    2817 
   Size:       1272    1545
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237    1376    1946    2042    2588    2719    2817 
   Size:        237    1139     570      96     546     131      98

  Fragments arranged by size: 

                1139     570     546     237     131      98      96
 
EcoRI G'AATT_C

Cuts at:      0     659    2817 
   Size:        659    2158
 
EcoRV GAT'ATC

Cuts at:      0     654    2817 
   Size:        654    2163
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     511    1520    2817 
   Size:        511    1009    1297

  Fragments arranged by size: 

                1297    1009     511
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1774    1806    2817 
   Size:       1774      32    1011

  Fragments arranged by size: 

                1774    1011      32
 
HincII GTy'rAC

Cuts at:      0    2691    2817 
   Size:       2691     126
 
HindIII A'AGCT_T

Cuts at:      0     243     974    1497    2324    2817 
   Size:        243     731     523     827     493

  Fragments arranged by size: 

                 827     731     523     493     243
 
HpaI GTT'AAC

Cuts at:      0    2691    2817 
   Size:       2691     126
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     270     969    1002    1011    2817 
   Size:        270     699      33       9    1806

  Fragments arranged by size: 

                1806     699     270      33       9
 
MscI TGG'CCA

Cuts at:      0      84    1859    2817 
   Size:         84    1775     958

  Fragments arranged by size: 

                1775     958      84
 
MslI CAynn'nnrTG

Cuts at:      0     376    1890    2654    2817 
   Size:        376    1514     764     163

  Fragments arranged by size: 

                1514     764     376     163
 
MspA1I CmG'CkG

Cuts at:      0      20     828    2817 
   Size:         20     808    1989

  Fragments arranged by size: 

                1989     808      20
 
NdeI CA'TA_TG

Cuts at:      0    2153    2303    2651    2817 
   Size:       2153     150     348     166

  Fragments arranged by size: 

                2153     348     166     150
 
NspI r_CATG'y

Cuts at:      0     427    2377    2817 
   Size:        427    1950     440

  Fragments arranged by size: 

                1950     440     427
 
PciI A'CATG_T

Cuts at:      0    2373    2817 
   Size:       2373     444
 
PsiI TTA'TAA

Cuts at:      0    1703    2138    2817 
   Size:       1703     435     679

  Fragments arranged by size: 

                1703     679     435
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     524     556    2817 
   Size:        524      32    2261

  Fragments arranged by size: 

                2261     524      32
 
PstI C_TGCA'G

Cuts at:      0     922    1767    1914    2067    2817 
   Size:        922     845     147     153     750

  Fragments arranged by size: 

                 922     845     750     153     147
 
PvuII CAG'CTG

Cuts at:      0     828    2817 
   Size:        828    1989
 
SacII CC_GC'GG

Cuts at:      0      21    2817 
   Size:         21    2796
 
ScaI AGT'ACT

Cuts at:      0    2814    2817 
   Size:       2814       3
 
SfcI C'TryA_G

Cuts at:      0     918    1763    1910    2063    2817 
   Size:        918     845     147     153     754

  Fragments arranged by size: 

                 918     845     754     153     147
 
SmlI C'TyrA_G

Cuts at:      0    1054    2817 
   Size:       1054    1763
 
SpeI A'CTAG_T

Cuts at:      0      70    2817 
   Size:         70    2747
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1006    1828    2817 
   Size:       1006     822     989

  Fragments arranged by size: 

                1006     989     822
 
TatI w'GTAC_w

Cuts at:      0    1097    2812    2817 
   Size:       1097    1715       5

  Fragments arranged by size: 

                1715    1097       5
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     800     832    2817 
   Size:        800      32    1985

  Fragments arranged by size: 

                1985     800      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     262    2147    2817 
   Size:        262    1885     670

  Fragments arranged by size: 

                1885     670     262
 
XmnI GAAnn'nnTTC

Cuts at:      0     307    1520    1551    2817 
   Size:        307    1213      31    1266

  Fragments arranged by size: 

                1266    1213     307      31
 

 Enzymes that do cut:

     AcuI    AflII   AflIII    AlwNI     ApoI     AseI     AvaI     BaeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BlpI     BmrI     BplI
   Bpu10I    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI     BsmI
    BspHI    BspMI   BssHII   BstAPI    BstBI   Bsu36I     BtgI     BtsI
   DraIII     EaeI     EarI     EciI  Eco57MI    EcoRI    EcoRV     FalI
    Hin4I   HincII  HindIII     HpaI     MmeI     MscI     MslI   MspA1I
     NdeI     NspI     PciI     PsiI     PsrI     PstI    PvuII    SacII
     ScaI     SfcI     SmlI     SpeI    TaqII     TatI     TstI  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
    BamHI     BanI    BanII     BbeI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BpmI    BpuEI     BsaI    BsaHI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI  EcoICRI    EcoNI EcoO109I     FseI
     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     StuI     StyI     SwaI     XbaI     XcmI
     XhoI     XmaI     ZraI
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