Restriction map for pF1KB7917
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        Asp718I     AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbrPI       
BciVI       BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DsaI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRV       EcoT14I     
EcoT22I     FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tth111I     
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI         1005  GAATTC               [GA]^AATT[CT]
AflIII        569  ACACGT               A^C[GA][CT]GT
AlwNI          28  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           89  GGGCCC               GGGCC^C
ApoI         1005  GAATTC               [GA]^AATT[CT]
AseI          931  ATTAAT               AT^TAAT
AsnI          931  ATTAAT               AT^TAAT
Asp700I       330  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI        1078  CCTCAGC              CCTCAGC(-5/-2)
BclI          524  TGATCA               T^GATCA
BglI           65  GCCGCTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI         654  CGTCTC               CGTCTC(1/5)
BsrDI         255  CATTGC               GCAATG(2/0)
BssHII         74  GCGCGC               G^CGCGC
BssSI         664  CACGAG               CACGAG(-5/-1)
BstEII        594  GGTCACC              G^GT[ACGT]ACC
BstPI         594  GGTCACC              G^GT[ACGT]ACC
BstYI         551  AGATCC               [GA]^GATC[CT]
DdeI         1079  CTCAG                C^T[ACGT]AG
DraII          88  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI          610  GACTGGCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           19  TGGCCG               [CT]^GGCC[GA]
EcoNI         600  CCTCCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       88  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       594  GGTCACC              G^GT[ACGT]ACC
EcoRI        1005  GAATTC               G^AATTC
FbaI          524  TGATCA               T^GATCA
HgaI           55  GCGTC                GACGC(5/10)
MflI          551  AGATCC               [GA]^GATC[CT]
NheI          990  GCTAGC               G^CTAGC
NspI          615  GCATGT               [GA]CATG^[CT]
PshBI         931  ATTAAT               AT^TAAT
PstI          604  CTGCAG               CTGCA^G
PvuII          31  CAGCTG               CAG^CTG
SbfI          603  CCTGCAGG             CCTGCA^GG
SexAI         179  ACCTGGT              A^CC[AT]GGT
SfcI          604  CTGCAG               C^T[GA][CT]AG
Sse8387I      603  CCTGCAGG             CCTGCA^GG
XbaI          777  TCTAGA               T^CTAGA
XhoII         551  AGATCC               [GA]^GATC[CT]
XmnI          330  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII          75  CGCG                 CG^CG
               94  CGCG                
AfaI          280  GTAC                 GT^AC
              573  GTAC                
AhdI           49  GACGAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              262  GACTTCTTGTC         
AlwI          150  GATCC                GGATC(4/5)
              552  GATCC               
AspEI          49  GACGAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              262  GACTTCTTGTC         
AspHI         141  GTGCTC               G[AT]GC[AT]^C
              355  GAGCAC              
BbeI            7  GGCGCC               GGCGC^C
             1043  GGCGCC              
BbsI          790  GAAGAC               GAAGAC(2/6)
              514  GTCTTC              
BcgI           96  CGAGCTGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1032  GCAAGAGCTTCG        
BfaI          778  CTAG                 C^TAG
              991  CTAG                
BpuAI         790  GAAGAC               GAAGAC(2/6)
              514  GTCTTC              
BsaAI         570  CACGTA               [CT]AC^GT[GA]
              629  CACGTA              
BsaI          400  GAGACC               GGTCTC(1/5)
              667  GAGACC              
BsiHKAI       141  GTGCTC               G[AT]GC[AT]^C
              355  GAGCAC              
BsmFI         410  GGGAC                GGGAC(10/14)
              700  GTCCC               
BstUI          75  CGCG                 CG^CG
               94  CGCG                
Eam1105I       49  GACGAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              262  GACTTCTTGTC         
KasI            7  GGCGCC               G^GCGCC
             1043  GGCGCC              
MaeI          778  CTAG                 C^TAG
              991  CTAG                
MseI          869  TTAA                 T^TAA
              932  TTAA                
MslI          283  CACTGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              981  CATATCAGTG          
MvnI           75  CGCG                 CG^CG
               94  CGCG                
NarI            7  GGCGCC               GG^CGCC
             1043  GGCGCC              
PflMI          27  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              174  CCACAACCTGG         
RsaI          280  GTAC                 GT^AC
              573  GTAC                
SfoI            7  GGCGCC               GGC^GCC
             1043  GGCGCC              
TaqI          398  TCGA                 T^CGA
              731  TCGA                
Tru9I         869  TTAA                 T^TAA
              932  TTAA                
Tsp45I        424  GTGAC                ^GT[GC]AC
              595  GTCAC               
TthHB8I       398  TCGA                 T^CGA
              731  TCGA                
Van91I         27  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              174  CCACAACCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AcyI        AluI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DpnII       EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp509I     TspRI       
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