Restriction map for pF1KB7917
 (Linear) (Six-base) MAPSORT of: KIBB7917.seq  Check: 2560  from: 1  to: 1110



KIBB7917.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     497    1110 
   Size:        144     353     613

  Fragments arranged by size: 

                 613     353     144
 
AflIII A'CryG_T

Cuts at:      0     569    1110 
   Size:        569     541
 
AhdI GACnn_n'nnGTC

Cuts at:      0      54     267    1110 
   Size:         54     213     843

  Fragments arranged by size: 

                 843     213      54
 
AleI CACnn'nnGTG

Cuts at:      0     287    1110 
   Size:        287     823
 
AlwNI CAG_nnn'CTG

Cuts at:      0      33    1110 
   Size:         33    1077
 
ApaI G_GGCC'C

Cuts at:      0      93    1110 
   Size:         93    1017
 
ApoI r'AATT_y

Cuts at:      0    1005    1110 
   Size:       1005     105
 
AseI AT'TA_AT

Cuts at:      0     932    1110 
   Size:        932     178
 
BanI G'GyrC_C

Cuts at:      0       7     535    1043    1110 
   Size:          7     528     508      67

  Fragments arranged by size: 

                 528     508      67       7
 
BanII G_rGCy'C

Cuts at:      0      93     252     307    1110 
   Size:         93     159      55     803

  Fragments arranged by size: 

                 803     159      93      55
 
BbeI G_GCGC'C

Cuts at:      0      11    1047    1110 
   Size:         11    1036      63

  Fragments arranged by size: 

                1036      63      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     507     797    1110 
   Size:        507     290     313

  Fragments arranged by size: 

                 507     313     290
 
BbvCI CC'TCA_GC

Cuts at:      0    1079    1110 
   Size:       1079      31
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      85     119    1021    1055    1110 
   Size:         85      34     902      34      55

  Fragments arranged by size: 

                 902      85      55      34      34
 
BclI T'GATC_A

Cuts at:      0     524    1110 
   Size:        524     586
 
BglI GCCn_nnn'nGGC

Cuts at:      0      71    1110 
   Size:         71    1039
 
Bme1580I G_kGCm'C

Cuts at:      0      93    1110 
   Size:         93    1017
 
BmgBI CAC'GTC

Cuts at:      0     654    1110 
   Size:        654     456
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     294    1110 
   Size:        294     816
 
BmtI G_CTAG'C

Cuts at:      0     994    1110 
   Size:        994     116
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     159    1110 
   Size:        159     951
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     115     505    1110 
   Size:         55      60     390     605

  Fragments arranged by size: 

                 605     390      60      55
 
Bpu10I CC'TnA_GC

Cuts at:      0    1079    1110 
   Size:       1079      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     394     661    1110 
   Size:        394     267     449

  Fragments arranged by size: 

                 449     394     267
 
BsaAI yAC'GTr

Cuts at:      0     572     631    1110 
   Size:        572      59     479

  Fragments arranged by size: 

                 572     479      59
 
BsaHI Gr'CG_yC

Cuts at:      0       8      55    1044    1110 
   Size:          8      47     989      66

  Fragments arranged by size: 

                 989      66      47       8
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     683     713    1110 
   Size:        683      30     397

  Fragments arranged by size: 

                 683     397      30
 
BseYI C'CCAG_C

Cuts at:      0      68     206     590     770    1110 
   Size:         68     138     384     180     340

  Fragments arranged by size: 

                 384     340     180     138      68
 
BsiHKAI G_wGCw'C

Cuts at:      0     145     359    1110 
   Size:        145     214     751

  Fragments arranged by size: 

                 751     214     145
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     660    1110 
   Size:        660     450
 
Bsp1286I G_dGCh'C

Cuts at:      0      93     145     252     307     359    1110 
   Size:         93      52     107      55      52     751

  Fragments arranged by size: 

                 751     107      93      55      52      52
 
BsrDI GCAATG_nn'

Cuts at:      0     254    1110 
   Size:        254     856
 
BssHII G'CGCG_C

Cuts at:      0      74    1110 
   Size:         74    1036
 
BssSI C'ACGA_G

Cuts at:      0     664    1110 
   Size:        664     446
 
BstEII G'GTnAC_C

Cuts at:      0     594    1110 
   Size:        594     516
 
BstYI r'GATC_y

Cuts at:      0     551    1110 
   Size:        551     559
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     153     625    1110 
   Size:        153     472     485

  Fragments arranged by size: 

                 485     472     153
 
BtsI GCAGTG_nn'

Cuts at:      0     427    1110 
   Size:        427     683
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     616    1110 
   Size:        616     494
 
EaeI y'GGCC_r

Cuts at:      0      19    1110 
   Size:         19    1091
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     115     144     497     505    1110 
   Size:         55      60      29     353       8     605

  Fragments arranged by size: 

                 605     353      60      55      29       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     604    1110 
   Size:        604     506
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89    1110 
   Size:         89    1021
 
EcoRI G'AATT_C

Cuts at:      0    1005    1110 
   Size:       1005     105
 
HaeII r_GCGC'y

Cuts at:      0      11     129    1047    1110 
   Size:         11     118     918      63

  Fragments arranged by size: 

                 918     118      63      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      40      72     253     285     683     715    1110 
   Size:         40      32     181      32     398      32     395

  Fragments arranged by size: 

                 398     395     181      40      32      32      32
 
KasI G'GCGC_C

Cuts at:      0       7    1043    1110 
   Size:          7    1036      67

  Fragments arranged by size: 

                1036      67       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     366     701     738    1110 
   Size:        366     335      37     372

  Fragments arranged by size: 

                 372     366     335      37
 
MslI CAynn'nnrTG

Cuts at:      0     287     985    1110 
   Size:        287     698     125

  Fragments arranged by size: 

                 698     287     125
 
MspA1I CmG'CkG

Cuts at:      0      33      68     206     438    1110 
   Size:         33      35     138     232     672

  Fragments arranged by size: 

                 672     232     138      35      33
 
NarI GG'CG_CC

Cuts at:      0       8    1044    1110 
   Size:          8    1036      66

  Fragments arranged by size: 

                1036      66       8
 
NheI G'CTAG_C

Cuts at:      0     990    1110 
   Size:        990     120
 
NspI r_CATG'y

Cuts at:      0     619    1110 
   Size:        619     491
 
PasI CC'CwG_GG

Cuts at:      0     500    1110 
   Size:        500     610
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      33     180    1110 
   Size:         33     147     930

  Fragments arranged by size: 

                 930     147      33
 
PfoI T'CCnGG_A

Cuts at:      0     131     350    1110 
   Size:        131     219     760

  Fragments arranged by size: 

                 760     219     131
 
PspOMI G'GGCC_C

Cuts at:      0      89    1110 
   Size:         89    1021
 
PstI C_TGCA'G

Cuts at:      0     608    1110 
   Size:        608     502
 
PvuII CAG'CTG

Cuts at:      0      33    1110 
   Size:         33    1077
 
SbfI CC_TGCA'GG

Cuts at:      0     608    1110 
   Size:        608     502
 
SexAI A'CCwGG_T

Cuts at:      0     179    1110 
   Size:        179     931
 
SfcI C'TryA_G

Cuts at:      0     604    1110 
   Size:        604     506
 
SfoI GGC'GCC

Cuts at:      0       9    1045    1110 
   Size:          9    1036      65

  Fragments arranged by size: 

                1036      65       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     481    1110 
   Size:        481     629
 
XbaI T'CTAG_A

Cuts at:      0     777    1110 
   Size:        777     333
 
XmnI GAAnn'nnTTC

Cuts at:      0     334    1110 
   Size:        334     776
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI     ApoI     AseI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaXI    BseYI  BsiHKAI    BsmBI Bsp1286I    BsrDI
   BssHII    BssSI   BstEII    BstYI    BtgZI     BtsI     DrdI     EaeI
  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I     KasI     MmeI
     MslI   MspA1I     NarI     NheI     NspI     PasI    PflMI     PfoI
   PspOMI     PstI    PvuII     SbfI    SexAI     SfcI     SfoI    TaqII
     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AlfI     AloI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BciVI    BfrBI    BglII     BlpI    BpuEI    BsaBI    BsaWI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     NaeI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI     ZraI
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