Restriction map for pF1KE0512
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BanI        BbeI        BbrPI       BbvCI       BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BseRI       BsiEI       BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MfeI        MluI        MslI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TfiI        Tsp509I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI           34  GTCTAC               GT^[AC][GT]AC
AccII         685  CGCG                 CG^CG
AocI          631  CCTGAGG              CC^T[ACGT]AGG
ApaI           15  GGGCCC               GGGCC^C
AspHI         523  GAGCTC               G[AT]GC[AT]^C
AvaI          526  CTCGGG               C^[CT]CG[GA]G
BamHI         504  GGATCC               G^GATCC
BbsI          118  GTCTTC               GAAGAC(2/6)
BcgI          518  GCACAGAGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI          499  CTCCAG               CTGGAG(16/14)
BpuAI         118  GTCTTC               GAAGAC(2/6)
BsiHKAI       523  GAGCTC               G[AT]GC[AT]^C
BsmFI         101  GTCCC                GGGAC(10/14)
BsoBI         526  CTCGGG               C^[CT]CG[GA]G
BspLU11I      590  ACATGT               A^CATGT
BstUI         685  CGCG                 CG^CG
BstYI         504  GGATCC               [GA]^GATC[CT]
Bsu36I        631  CCTGAGG              CC^T[ACGT]AGG
DraIII        765  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        631  CCTGAGG              CC^T[ACGT]AGG
EcoNI         372  CCTGGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          738  GCGGG                CCCGC(4/6)
MaeIII          7  GTGAC                ^GT[ACGT]AC
MboII         119  TCTTC                GAAGA(8/7)
MflI          504  GGATCC               [GA]^GATC[CT]
MseI          611  TTAA                 T^TAA
MvnI          685  CGCG                 CG^CG
PflMI         150  CCAGACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         697  CAGCTG               CAG^CTG
SacI          523  GAGCTC               GAGCT^C
ScaI          830  AGTACT               AGT^ACT
SexAI         596  ACCAGGT              A^CC[AT]GGT
SphI          177  GCATGC               GCATG^C
StuI          452  AGGCCT               AGG^CCT
Tru9I         611  TTAA                 T^TAA
Tsp45I          7  GTGAC                ^GT[GC]AC
Van91I        150  CCAGACAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          331  CCAGACAAGGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         504  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccIII         83  TCCGGA               T^CCGGA
              317  TCCGGA              
AflIII         92  ACACGT               A^C[GA][CT]GT
              590  ACATGT              
AlwNI         694  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              712  CAGGACCTG           
BalI           62  TGGCCA               TGG^CCA
              719  TGGCCA              
BcnI          264  CCCGG                CC^[GC]GG
              774  CCCGG               
BseAI          83  TCCGGA               T^CCGGA
              317  TCCGGA              
BsgI          364  CTGCAC               GTGCAG(16/14)
              702  GTGCAG              
BspEI          83  TCCGGA               T^CCGGA
              317  TCCGGA              
BspMI         233  ACCTGC               ACCTGC(4/8)
              779  ACCTGC              
DdeI          632  CTGAG                C^T[ACGT]AG
              847  CTGAG               
DpnII         505  GATC                 ^GATC
              648  GATC                
DsaI          227  CCGTGG               C^C[GA][CT]GG
              458  CCGTGG              
EaeI           62  TGGCCA               [CT]^GGCC[GA]
              719  TGGCCA              
HinfI         256  GAGTC                G^A[ACGT]TC
              472  GACTC               
MboI          505  GATC                 ^GATC
              648  GATC                
MluNI          62  TGGCCA               TGG^CCA
              719  TGGCCA              
MroI           83  TCCGGA               T^CCGGA
              317  TCCGGA              
MscI           62  TGGCCA               TGG^CCA
              719  TGGCCA              
MspA1I        305  CAGCGG               C[AC]G^C[GT]G
              697  CAGCTG              
NciI          264  CCCGG                CC^[GC]GG
              774  CCCGG               
NdeII         505  GATC                 ^GATC
              648  GATC                
NspI          177  GCATGC               [GA]CATG^[CT]
              590  ACATGT              
PleI          472  GACTC                GAGTC(4/5)
              256  GAGTC               
Sau3AI        505  GATC                 ^GATC
              648  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspI        
AvaII       BanII       BbvI        BmyI        BsaJI       
BsaWI       BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HpaII       HphI        ItaI        
MaeII       MnlI        MspI        MvaI        MwoI        
NlaIII      NlaIV       PflFI       PpuMI       RsaI        
Sau96I      ScrFI       SfaNI       TaqI        TseI        
TspRI       Tth111I     TthHB8I     
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