Restriction map for pF1KB7012
 (Linear) (Six-base) MAPSORT of: KIBB7012.seq  Check: 9313  from: 1  to: 3120



KIBB7012.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      38     156     480    1468    1953    3120 
   Size:         38     118     324     988     485    1167

  Fragments arranged by size: 

                1167     988     485     324     118      38
 
AccI GT'mk_AC

Cuts at:      0     323    2315    3120 
   Size:        323    1992     805

  Fragments arranged by size: 

                1992     805     323
 
Acc65I G'GTAC_C

Cuts at:      0     229    1204    3120 
   Size:        229     975    1916

  Fragments arranged by size: 

                1916     975     229
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     645     713    1239    2942    3120 
   Size:         50     595      68     526    1703     178

  Fragments arranged by size: 

                1703     595     526     178      68      50
 
AflIII A'CryG_T

Cuts at:      0    1754    3120 
   Size:       1754    1366
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1785    3120 
   Size:       1785    1335
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     982    3120 
   Size:        982    2138
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1126    1158    2029    2061    3120 
   Size:       1126      32     871      32    1059

  Fragments arranged by size: 

                1126    1059     871      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     333    1012    2016    2920    3120 
   Size:        333     679    1004     904     200

  Fragments arranged by size: 

                1004     904     679     333     200
 
ApaI G_GGCC'C

Cuts at:      0    2096    2393    2966    3120 
   Size:       2096     297     573     154

  Fragments arranged by size: 

                2096     573     297     154
 
ApoI r'AATT_y

Cuts at:      0     400    2948    3120 
   Size:        400    2548     172

  Fragments arranged by size: 

                2548     400     172
 
AvaI C'yCGr_G

Cuts at:      0     188    1167    1325    2282    2392    2618    3120 
   Size:        188     979     158     957     110     226     502

  Fragments arranged by size: 

                 979     957     502     226     188     158     110
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2194    2227    3120 
   Size:       2194      33     893

  Fragments arranged by size: 

                2194     893      33
 
BamHI G'GATC_C

Cuts at:      0      88    2978    3120 
   Size:         88    2890     142

  Fragments arranged by size: 

                2890     142      88
 
BanI G'GyrC_C

Cuts at:      0     229     352     904    1204    1411    2136    2286    2293 
   Size:        229     123     552     300     207     725     150       7

Cuts at:   2293    3064    3120 
   Size:        771      56

  Fragments arranged by size: 

                 771     725     552     300     229     207     150     123
                  56       7
 
BanII G_rGCy'C

Cuts at:      0     866    2096    2169    2180    2393    2435    2966    3119 
   Size:        866    1230      73      11     213      42     531     153

Cuts at:   3119    3120 
   Size:          1

  Fragments arranged by size: 

                1230     866     531     213     153      73      42      11
                   1
 
BbeI G_GCGC'C

Cuts at:      0    2297    3120 
   Size:       2297     823
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     125     651    1169    2930    3120 
   Size:        125     526     518    1761     190

  Fragments arranged by size: 

                1761     526     518     190     125
 
BbvCI CC'TCA_GC

Cuts at:      0    2423    3120 
   Size:       2423     697
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1978    2012    2111    2145    3120 
   Size:       1978      34      99      34     975

  Fragments arranged by size: 

                1978     975      99      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2536    3120 
   Size:       2536     584
 
BclI T'GATC_A

Cuts at:      0    1418    3120 
   Size:       1418    1702
 
BglI GCCn_nnn'nGGC

Cuts at:      0     984    1671    2091    3120 
   Size:        984     687     420    1029

  Fragments arranged by size: 

                1029     984     687     420
 
BlpI GC'TnA_GC

Cuts at:      0     674     860     974    1153    1876    3120 
   Size:        674     186     114     179     723    1244

  Fragments arranged by size: 

                1244     723     674     186     179     114
 
Bme1580I G_kGCm'C

Cuts at:      0     355     907    1676    2077    2096    2393    2619    2966 
   Size:        355     552     769     401      19     297     226     347

Cuts at:   2966    3120 
   Size:        154

  Fragments arranged by size: 

                 769     552     401     355     347     297     226     154
                  19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     340     418    2087    2298    2696    2908    3120 
   Size:        340      78    1669     211     398     212     212

  Fragments arranged by size: 

                1669     398     340     212     212     211      78
 
BmtI G_CTAG'C

Cuts at:      0    1231    3120 
   Size:       1231    1889
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     265     796    1374    1388    1688    1902    2082 
   Size:        185      80     531     578      14     300     214     180

Cuts at:   2082    3120 
   Size:       1038

  Fragments arranged by size: 

                1038     578     531     300     214     185     180      80
                  14
 
Bpu10I CC'TnA_GC

Cuts at:      0    2423    3120 
   Size:       2423     697
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1530    2754    3120 
   Size:       1530    1224     366

  Fragments arranged by size: 

                1530    1224     366
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     413     736    1693    2475    3120 
   Size:        413     323     957     782     645

  Fragments arranged by size: 

                 957     782     645     413     323
 
BsaAI yAC'GTr

Cuts at:      0    1757    3120 
   Size:       1757    1363
 
BsaBI GATnn'nnATC

Cuts at:      0    1309    2908    3094    3120 
   Size:       1309    1599     186      26

  Fragments arranged by size: 

                1599    1309     186      26
 
BsaHI Gr'CG_yC

Cuts at:      0     543    1275    2194    2294    3120 
   Size:        543     732     919     100     826

  Fragments arranged by size: 

                 919     826     732     543     100
 
BsaWI w'CCGG_w

Cuts at:      0    1679    2108    3120 
   Size:       1679     429    1012

  Fragments arranged by size: 

                1679    1012     429
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     154     184    1006    1036    1386    1416    1826    1856 
   Size:        154      30     822      30     350      30     410      30

Cuts at:   1856    3120 
   Size:       1264

  Fragments arranged by size: 

                1264     822     410     350     154      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      51     163     175     427     496    2171    3120 
   Size:         51     112      12     252      69    1675     949

  Fragments arranged by size: 

                1675     949     252     112      69      51      12
 
BseYI C'CCAG_C

Cuts at:      0     443     715     735     880    1129    1730    2016    2560 
   Size:        443     272      20     145     249     601     286     544

Cuts at:   2560    3048    3120 
   Size:        488      72

  Fragments arranged by size: 

                 601     544     488     443     286     272     249     145
                  72      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     970     988    1064    1991    2390    2527    3120 
   Size:        970      18      76     927     399     137     593

  Fragments arranged by size: 

                 970     927     593     399     137      76      18
 
BsiHKAI G_wGCw'C

Cuts at:      0    1352    2180    3119    3120 
   Size:       1352     828     939       1

  Fragments arranged by size: 

                1352     939     828       1
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2216    3120 
   Size:       2216     904
 
Bsp1286I G_dGCh'C

Cuts at:      0     355     866     907    1352    1676    2077    2096    2169 
   Size:        355     511      41     445     324     401      19      73

Cuts at:   2169    2180    2393    2435    2619    2966    3119    3120 
   Size:         11     213      42     184     347     153       1

  Fragments arranged by size: 

                 511     445     401     355     347     324     213     184
                 153      73      42      41      19      11       1
 
BspEI T'CCGG_A

Cuts at:      0    2108    3120 
   Size:       2108    1012
 
BspHI T'CATG_A

Cuts at:      0     290     435    3120 
   Size:        290     145    2685

  Fragments arranged by size: 

                2685     290     145
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      38     156     480     756    1037    1145    1468    1953 
   Size:         38     118     324     276     281     108     323     485

Cuts at:   1953    2246    3120 
   Size:        293     874

  Fragments arranged by size: 

                 874     485     324     323     293     281     276     118
                 108      38
 
BsrDI GCAATG_nn'

Cuts at:      0     578    2001    3120 
   Size:        578    1423    1119

  Fragments arranged by size: 

                1423    1119     578
 
BsrFI r'CCGG_y

Cuts at:      0    1051    1436    2833    2890    3120 
   Size:       1051     385    1397      57     230

  Fragments arranged by size: 

                1397    1051     385     230      57
 
BsrGI T'GTAC_A

Cuts at:      0     575    3120 
   Size:        575    2545
 
BssSI C'ACGA_G

Cuts at:      0    2410    3120 
   Size:       2410     710
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     171     313    3120 
   Size:        171     142    2807

  Fragments arranged by size: 

                2807     171     142
 
BstEII G'GTnAC_C

Cuts at:      0     768    3120 
   Size:        768    2352
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     348     636    1806    3120 
   Size:        348     288    1170    1314

  Fragments arranged by size: 

                1314    1170     348     288
 
BstYI r'GATC_y

Cuts at:      0      88     875    1304    1710    2360    2543    2978    3120 
   Size:         88     787     429     406     650     183     435     142

  Fragments arranged by size: 

                 787     650     435     429     406     183     142      88
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    2142    3120 
   Size:       2142     978
 
BtgI C'CryG_G

Cuts at:      0     155    1286    1293    1343    1610    1740    1887    2379 
   Size:        155    1131       7      50     267     130     147     492

Cuts at:   2379    2734    3083    3120 
   Size:        355     349      37

  Fragments arranged by size: 

                1131     492     355     349     267     155     147     130
                  50      37       7
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1896    3103    3120 
   Size:       1896    1207      17

  Fragments arranged by size: 

                1896    1207      17
 
BtsI GCAGTG_nn'

Cuts at:      0     853    2402    3120 
   Size:        853    1549     718

  Fragments arranged by size: 

                1549     853     718
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1325    1360    3120 
   Size:       1325      35    1760

  Fragments arranged by size: 

                1760    1325      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     552    3000    3084    3120 
   Size:        552    2448      84      36

  Fragments arranged by size: 

                2448     552      84      36
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2199    2803    3120 
   Size:       2199     604     317

  Fragments arranged by size: 

                2199     604     317
 
EaeI y'GGCC_r

Cuts at:      0     623    2931    3120 
   Size:        623    2308     189

  Fragments arranged by size: 

                2308     623     189
 
EarI CTCTTCn'nnn_

Cuts at:      0      69    2423    3120 
   Size:         69    2354     697

  Fragments arranged by size: 

                2354     697      69
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     937    1808    3034    3120 
   Size:        937     871    1226      86

  Fragments arranged by size: 

                1226     937     871      86
 
EcoICRI GAG'CTC

Cuts at:      0    2178    3117    3120 
   Size:       2178     939       3

  Fragments arranged by size: 

                2178     939       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     185     265     645     713     796    1239    1374 
   Size:         50     135      80     380      68      83     443     135

Cuts at:   1374    1388    1688    1902    2082    2942    3120 
   Size:         14     300     214     180     860     178

  Fragments arranged by size: 

                 860     443     380     300     214     180     178     135
                 135      83      80      68      50      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2250    3120 
   Size:       2250     870
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1134    1825    2093    2666    2796    2962    3120 
   Size:       1134     691     268     573     130     166     158

  Fragments arranged by size: 

                1134     691     573     268     166     158     130
 
EcoRI G'AATT_C

Cuts at:      0     400    3120 
   Size:        400    2720
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     675    3120 
   Size:        675    2445
 
HaeII r_GCGC'y

Cuts at:      0    1223    2297    3120 
   Size:       1223    1074     823

  Fragments arranged by size: 

                1223    1074     823
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1006    1038    1386    1418    1771    1803    2165    2197 
   Size:       1006      32     348      32     353      32     362      32

Cuts at:   2197    3120 
   Size:        923

  Fragments arranged by size: 

                1006     923     362     353     348      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1380    2187    3120 
   Size:       1380     807     933

  Fragments arranged by size: 

                1380     933     807
 
KasI G'GCGC_C

Cuts at:      0    2293    3120 
   Size:       2293     827
 
KpnI G_GTAC'C

Cuts at:      0     233    1208    3120 
   Size:        233     975    1912

  Fragments arranged by size: 

                1912     975     233
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365    1049    3120 
   Size:        365     684    2071

  Fragments arranged by size: 

                2071     684     365
 
MscI TGG'CCA

Cuts at:      0     625    2933    3120 
   Size:        625    2308     187

  Fragments arranged by size: 

                2308     625     187
 
MslI CAynn'nnrTG

Cuts at:      0     964    1759    2719    3019    3025    3120 
   Size:        964     795     960     300       6      95

  Fragments arranged by size: 

                 964     960     795     300      95       6
 
MspA1I CmG'CkG

Cuts at:      0     267     500     859    1288    1295    1734    1880    2153 
   Size:        267     233     359     429       7     439     146     273

Cuts at:   2153    2381    2422    2578    2627    2736    3120 
   Size:        228      41     156      49     109     384

  Fragments arranged by size: 

                 439     429     384     359     273     267     233     228
                 156     146     109      49      41       7
 
NaeI GCC'GGC

Cuts at:      0    1053    3120 
   Size:       1053    2067
 
NarI GG'CG_CC

Cuts at:      0    2294    3120 
   Size:       2294     826
 
NcoI C'CATG_G

Cuts at:      0    1610    1740    3120 
   Size:       1610     130    1380

  Fragments arranged by size: 

                1610    1380     130
 
NgoMIV G'CCGG_C

Cuts at:      0    1051    3120 
   Size:       1051    2069
 
NheI G'CTAG_C

Cuts at:      0    1227    3120 
   Size:       1227    1893
 
NspI r_CATG'y

Cuts at:      0     182    1176    3120 
   Size:        182     994    1944

  Fragments arranged by size: 

                1944     994     182
 
PasI CC'CwG_GG

Cuts at:      0      84    2435    3120 
   Size:         84    2351     685

  Fragments arranged by size: 

                2351     685      84
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     270     333    1185    1773    2456    2940    3061    3084 
   Size:        270      63     852     588     683     484     121      23

Cuts at:   3084    3120 
   Size:         36

  Fragments arranged by size: 

                 852     683     588     484     270     121      63      36
                  23
 
PfoI T'CCnGG_A

Cuts at:      0    2758    3120 
   Size:       2758     362
 
PmlI CAC'GTG

Cuts at:      0    1757    3120 
   Size:       1757    1363
 
PpuMI rG'GwC_Cy

Cuts at:      0    1134    1825    2796    3120 
   Size:       1134     691     971     324

  Fragments arranged by size: 

                1134     971     691     324
 
PshAI GACnn'nnGTC

Cuts at:      0     542    3120 
   Size:        542    2578
 
PspOMI G'GGCC_C

Cuts at:      0    2092    2389    2962    3120 
   Size:       2092     297     573     158

  Fragments arranged by size: 

                2092     573     297     158
 
PstI C_TGCA'G

Cuts at:      0     398     875    1950    2829    2988    3120 
   Size:        398     477    1075     879     159     132

  Fragments arranged by size: 

                1075     879     477     398     159     132
 
PvuII CAG'CTG

Cuts at:      0     267     859    1734    1880    2422    2578    3120 
   Size:        267     592     875     146     542     156     542

  Fragments arranged by size: 

                 875     592     542     542     267     156     146
 
RsrII CG'GwC_CG

Cuts at:      0    2583    3120 
   Size:       2583     537
 
SacI G_AGCT'C

Cuts at:      0    2180    3119    3120 
   Size:       2180     939       1

  Fragments arranged by size: 

                2180     939       1
 
SacII CC_GC'GG

Cuts at:      0    1289    1296    2382    2737    3120 
   Size:       1289       7    1086     355     383

  Fragments arranged by size: 

                1289    1086     383     355       7
 
SanDI GG'GwC_CC

Cuts at:      0    2796    3120 
   Size:       2796     324
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2423    3120 
   Size:       2423     697
 
SbfI CC_TGCA'GG

Cuts at:      0    1950    3120 
   Size:       1950    1170
 
ScaI AGT'ACT

Cuts at:      0    2308    3120 
   Size:       2308     812
 
SexAI A'CCwGG_T

Cuts at:      0     281    3120 
   Size:        281    2839
 
SfcI C'TryA_G

Cuts at:      0     394     871    1067    1582    1946    2316    2825    2984 
   Size:        394     477     196     515     364     370     509     159

Cuts at:   2984    3120 
   Size:        136

  Fragments arranged by size: 

                 515     509     477     394     370     364     196     159
                 136
 
SfoI GGC'GCC

Cuts at:      0    2295    3120 
   Size:       2295     825
 
SmaI CCC'GGG

Cuts at:      0     190    1327    2284    3120 
   Size:        190    1137     957     836

  Fragments arranged by size: 

                1137     957     836     190
 
SmlI C'TyrA_G

Cuts at:      0    1545    2769    3120 
   Size:       1545    1224     351

  Fragments arranged by size: 

                1545    1224     351
 
SphI G_CATG'C

Cuts at:      0     182    3120 
   Size:        182    2938
 
SrfI GCCC'GGGC

Cuts at:      0     190    3120 
   Size:        190    2930
 
StyI C'CwwG_G

Cuts at:      0     302     407     932    1610    1740    1790    2450    3120 
   Size:        302     105     525     678     130      50     660     670

  Fragments arranged by size: 

                 678     670     660     525     302     130     105      50
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     729    1555    2600    3120 
   Size:        729     826    1045     520

  Fragments arranged by size: 

                1045     826     729     520
 
TatI w'GTAC_w

Cuts at:      0     575    1751    1919    2306    2414    3120 
   Size:        575    1176     168     387     108     706

  Fragments arranged by size: 

                1176     706     575     387     168     108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     538     570    1826    1858    3120 
   Size:        538      32    1256      32    1262

  Fragments arranged by size: 

                1262    1256     538      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     633    1155    1738    2067    3120 
   Size:        633     522     583     329    1053

  Fragments arranged by size: 

                1053     633     583     522     329
 
XmaI C'CCGG_G

Cuts at:      0     188    1325    2282    3120 
   Size:        188    1137     957     838

  Fragments arranged by size: 

                1137     957     838     188
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AhdI     AlfI     AloI
    AlwNI     ApaI     ApoI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI
 Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NspI     PasI    PflMI     PfoI     PmlI
    PpuMI    PshAI   PspOMI     PstI    PvuII    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SmaI
     SmlI     SphI     SrfI     StyI    TaqII     TatI     TstI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AleI    ApaLI     AscI
     AseI    AsiSI    AvrII    BfrBI    BglII    BmgBI     BplI    BsiEI
    BsiWI     BsmI    BsrBI   BssHII    BstBI  BstZ17I     ClaI     DraI
     EagI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     MfeI
     MluI     NdeI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PpiI     PsiI    PspXI     PsrI     PvuI     SalI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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