(Circular) (Six-base) MAPSORT of: pF1KB7615.seq Check: 5063 from: 1 to: 4025 pF1KB7615 4025 bp With 182 enzymes: SgfI * June 26, 2008 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 582 582 Size: 4025 AccI GT'mk_AC Cuts at: 1063 1063 Size: 4025 Acc65I G'GTAC_C Cuts at: 1041 1041 Size: 4025 AclI AA'CG_TT Cuts at: 3865 3865 Size: 4025 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1850 2282 3029 3297 1850 Size: 432 747 268 2578 Fragments arranged by size: 2578 747 432 268 AflIII A'CryG_T Cuts at: 1377 2481 3458 1377 Size: 1104 977 1944 Fragments arranged by size: 1944 1104 977 AgeI A'CCGG_T Cuts at: 2413 2413 Size: 4025 AhdI GACnn_n'nnGTC Cuts at: 213 213 Size: 4025 AleI CACnn'nnGTG Cuts at: 989 989 Size: 4025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 878 878 Size: 4025 AlwNI CAG_nnn'CTG Cuts at: 369 431 567 1330 2897 369 Size: 62 136 763 1567 1497 Fragments arranged by size: 1567 1497 763 136 62 ApaI G_GGCC'C Cuts at: 458 458 Size: 4025 ApaLI G'TGCA_C Cuts at: 2795 2795 Size: 4025 ApoI r'AATT_y Cuts at: 721 1029 3378 3634 721 Size: 308 2349 256 1112 Fragments arranged by size: 2349 1112 308 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4025 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4025 AvaI C'yCGr_G Cuts at: 223 869 1045 223 Size: 646 176 3203 Fragments arranged by size: 3203 646 176 BamHI G'GATC_C Cuts at: 1050 1050 Size: 4025 BanI G'GyrC_C Cuts at: 308 383 1041 1455 1703 1738 308 Size: 75 658 414 248 35 2595 Fragments arranged by size: 2595 658 414 248 75 35 BanII G_rGCy'C Cuts at: 230 458 514 1039 2069 230 Size: 228 56 525 1030 2186 Fragments arranged by size: 2186 1030 525 228 56 BbeI G_GCGC'C Cuts at: 1459 1707 1459 Size: 248 3777 BbsI GAAGACnn'nnnn_ Cuts at: 392 983 392 Size: 591 3434 BciVI GTATCCnnnnn_n' Cuts at: 256 1923 2684 4025 256 Size: 1667 761 1341 256 Fragments arranged by size: 1667 1341 761 256 BclI T'GATC_A Cuts at: 1545 1545 Size: 4025 BglII A'GATC_T Cuts at: 202 1540 3577 202 Size: 1338 2037 650 Fragments arranged by size: 2037 1338 650 BlpI GC'TnA_GC Cuts at: 1137 1137 Size: 4025 Bme1580I G_kGCm'C Cuts at: 311 458 1650 1743 2799 311 Size: 147 1192 93 1056 1537 Fragments arranged by size: 1537 1192 1056 147 93 BmrI ACTGGGnnnn_n' Cuts at: 536 1237 1318 1653 3797 536 Size: 701 81 335 2144 764 Fragments arranged by size: 2144 764 701 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 434 2392 434 Size: 1958 2067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 421 470 498 931 421 Size: 49 28 433 3515 Fragments arranged by size: 3515 433 49 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1201 2080 2572 2870 1201 Size: 879 492 298 2356 Fragments arranged by size: 2356 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3729 3729 Size: 4025 BsaAI yAC'GTr Cuts at: 1378 1378 Size: 4025 BsaBI GATnn'nnATC Cuts at: 6 1539 3576 6 Size: 1533 2037 455 Fragments arranged by size: 2037 1533 455 BsaHI Gr'CG_yC Cuts at: 1456 1704 2406 3902 1456 Size: 248 702 1496 1579 Fragments arranged by size: 1579 1496 702 248 BsaWI w'CCGG_w Cuts at: 1214 1735 2413 2687 2834 3245 1214 Size: 521 678 274 147 411 1994 Fragments arranged by size: 1994 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 157 187 157 Size: 30 3995 BseYI C'CCAG_C Cuts at: 1450 2208 2785 3281 1450 Size: 758 577 496 2194 Fragments arranged by size: 2194 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 956 956 Size: 4025 BsiEI CG_ry'CG Cuts at: 104 558 1567 1613 2821 104 Size: 454 1009 46 1208 1308 Fragments arranged by size: 1308 1208 1009 454 46 BsiHKAI G_wGCw'C Cuts at: 230 493 1039 1817 2007 2799 230 Size: 263 546 778 190 792 1456 Fragments arranged by size: 1456 792 778 546 263 190 Bsp1286I G_dGCh'C Cuts at: 230 311 458 493 514 1039 1650 1743 1817 Size: 81 147 35 21 525 611 93 74 Cuts at: 1817 2007 2069 2799 230 Size: 190 62 730 1456 Fragments arranged by size: 1456 730 611 525 190 147 93 81 74 62 35 21 BspEI T'CCGG_A Cuts at: 3245 3245 Size: 4025 BspHI T'CATG_A Cuts at: 3201 3201 Size: 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 582 1075 1289 1591 1972 582 Size: 493 214 302 381 2635 Fragments arranged by size: 2635 493 381 302 214 BsrBI CCG'CTC Cuts at: 50 120 2317 2371 3855 4020 50 Size: 70 2197 54 1484 165 55 Fragments arranged by size: 2197 1484 165 70 55 54 BsrDI GCAATG_nn' Cuts at: 1937 1937 Size: 4025 BsrFI r'CCGG_y Cuts at: 2023 2204 2413 2023 Size: 181 209 3635 Fragments arranged by size: 3635 209 181 BssHII G'CGCG_C Cuts at: 539 539 Size: 4025 BssSI C'ACGA_G Cuts at: 2296 2654 2296 Size: 358 3667 BstAPI GCAn_nnn'nTGC Cuts at: 162 724 1402 162 Size: 562 678 2785 Fragments arranged by size: 2785 678 562 BstBI TT'CG_AA Cuts at: 2386 2386 Size: 4025 BstYI r'GATC_y Cuts at: 1 202 780 1050 1517 1540 1875 2121 3133 Size: 201 578 270 467 23 335 246 1012 Cuts at: 3133 3219 3231 3577 1 Size: 86 12 346 449 Fragments arranged by size: 1012 578 467 449 346 335 270 246 201 86 23 12 BtgI C'CryG_G Cuts at: 107 149 195 107 Size: 42 46 3937 Fragments arranged by size: 3937 46 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1975 2156 2319 1975 Size: 181 163 3681 Fragments arranged by size: 3681 181 163 BtsI GCAGTG_nn' Cuts at: 776 1268 776 Size: 492 3533 DraI TTT'AAA Cuts at: 1024 1024 Size: 4025 DraIII CAC_nnn'GTG Cuts at: 412 412 Size: 4025 DrdI GACnn_nn'nnGTC Cuts at: 1731 2589 1731 Size: 858 3167 EaeI y'GGCC_r Cuts at: 555 1610 1784 2175 2202 3547 555 Size: 1055 174 391 27 1345 1033 Fragments arranged by size: 1345 1055 1033 391 174 27 EagI C'GGCC_G Cuts at: 555 1610 555 Size: 1055 2970 EarI CTCTTCn'nnn_ Cuts at: 269 504 829 2048 2258 269 Size: 235 325 1219 210 2036 Fragments arranged by size: 2036 1219 325 235 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 598 837 2233 2543 2689 3835 598 Size: 239 1396 310 146 1146 788 Fragments arranged by size: 1396 1146 788 310 239 146 EcoICRI GAG'CTC Cuts at: 228 1037 228 Size: 809 3216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 421 470 498 931 1850 2282 3029 3297 421 Size: 49 28 433 919 432 747 268 1149 Fragments arranged by size: 1149 919 747 433 432 268 49 28 EcoO109I rG'GnC_Cy Cuts at: 454 455 1164 454 Size: 1 709 3315 Fragments arranged by size: 3315 709 1 EcoRI G'AATT_C Cuts at: 1029 1029 Size: 4025 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2044 2044 Size: 4025 FspI TGC'GCA Cuts at: 136 1013 1806 136 Size: 877 793 2355 Fragments arranged by size: 2355 877 793 FspAI rTGC'GCAy Cuts at: 1013 1013 Size: 4025 HaeII r_GCGC'y Cuts at: 1459 1707 2729 3708 1459 Size: 248 1022 979 1776 Fragments arranged by size: 1776 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 199 231 985 1017 1867 1897 1899 1929 199 Size: 32 754 32 850 30 2 30 2295 Fragments arranged by size: 2295 850 754 32 32 30 30 2 HincII GTy'rAC Cuts at: 657 1064 657 Size: 407 3618 KasI G'GCGC_C Cuts at: 1455 1703 1455 Size: 248 3777 KpnI G_GTAC'C Cuts at: 1045 1045 Size: 4025 MluI A'CGCG_T Cuts at: 3458 3458 Size: 4025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 518 2696 2880 518 Size: 2178 184 1663 Fragments arranged by size: 2178 1663 184 MscI TGG'CCA Cuts at: 1786 1786 Size: 4025 MslI CAynn'nnrTG Cuts at: 989 2014 2141 989 Size: 1025 127 2873 Fragments arranged by size: 2873 1025 127 MspA1I CmG'CkG Cuts at: 1136 1450 1810 2823 3068 1136 Size: 314 360 1013 245 2093 Fragments arranged by size: 2093 1013 360 314 245 NaeI GCC'GGC Cuts at: 2206 2206 Size: 4025 NarI GG'CG_CC Cuts at: 1456 1704 1456 Size: 248 3777 NcoI C'CATG_G Cuts at: 107 195 107 Size: 88 3937 NdeI CA'TA_TG Cuts at: 212 212 Size: 4025 NgoMIV G'CCGG_C Cuts at: 2204 2204 Size: 4025 NspI r_CATG'y Cuts at: 285 1012 1078 1578 2485 285 Size: 727 66 500 907 1825 Fragments arranged by size: 1825 907 727 500 66 PciI A'CATG_T Cuts at: 2481 2481 Size: 4025 PmeI GTTT'AAAC Cuts at: 1024 1024 Size: 4025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 104 136 3164 3196 104 Size: 32 3028 32 933 Fragments arranged by size: 3028 933 32 32 PspOMI G'GGCC_C Cuts at: 454 454 Size: 4025 PstI C_TGCA'G Cuts at: 1072 1757 1072 Size: 685 3340 PvuI CG_AT'CG Cuts at: 104 104 Size: 4025 PvuII CAG'CTG Cuts at: 1450 1810 1450 Size: 360 3665 RsrII CG'GwC_CG Cuts at: 2220 2220 Size: 4025 SacI G_AGCT'C Cuts at: 230 1039 230 Size: 809 3216 SalI G'TCGA_C Cuts at: 1062 1062 Size: 4025 SapI GCTCTTCn'nnn_ Cuts at: 2048 2258 2048 Size: 210 3815 SbfI CC_TGCA'GG Cuts at: 1072 1072 Size: 4025 ScaI AGT'ACT Cuts at: 3542 3542 Size: 4025 SfcI C'TryA_G Cuts at: 33 965 1068 1753 2746 2937 33 Size: 932 103 685 993 191 1121 Fragments arranged by size: 1121 993 932 685 191 103 SfoI GGC'GCC Cuts at: 1457 1705 1457 Size: 248 3777 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4025 SmaI CCC'GGG Cuts at: 1047 1047 Size: 4025 SmlI C'TyrA_G Cuts at: 1180 2095 2587 2849 1180 Size: 915 492 262 2356 Fragments arranged by size: 2356 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4025 SphI G_CATG'C Cuts at: 1012 1078 1578 1012 Size: 66 500 3459 Fragments arranged by size: 3459 500 66 StyI C'CwwG_G Cuts at: 107 195 1159 1513 107 Size: 88 964 354 2619 Fragments arranged by size: 2619 964 354 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1608 1741 2200 2409 1608 Size: 133 459 209 3224 Fragments arranged by size: 3224 459 209 133 TatI w'GTAC_w Cuts at: 3540 3540 Size: 4025 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 157 189 3542 3574 157 Size: 32 3353 32 608 Fragments arranged by size: 3353 608 32 32 Tth111I GACn'n_nGTC Cuts at: 1822 1822 Size: 4025 XbaI T'CTAG_A Cuts at: 1056 1056 Size: 4025 XcmI CCAnnnn_n'nnnnTGG Cuts at: 445 1520 445 Size: 1075 2950 XmaI C'CCGG_G Cuts at: 1045 1045 Size: 4025 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AloI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI HindIII HpaI MfeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI