(Circular) (Six-base) MAPSORT of: pF1KB6878.seq Check: 9652 from: 1 to: 3713 pF1KB6878 3713 bp With 182 enzymes: SgfI * June 26, 2008 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 237 237 Size: 3713 AccI GT'mk_AC Cuts at: 751 751 Size: 3713 Acc65I G'GTAC_C Cuts at: 729 729 Size: 3713 AclI AA'CG_TT Cuts at: 3553 3553 Size: 3713 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 193 1538 1970 2717 2985 193 Size: 1345 432 747 268 921 Fragments arranged by size: 1345 921 747 432 268 AflIII A'CryG_T Cuts at: 1065 2169 3146 1065 Size: 1104 977 1632 Fragments arranged by size: 1632 1104 977 AgeI A'CCGG_T Cuts at: 2101 2101 Size: 3713 AhdI GACnn_n'nnGTC Cuts at: 186 186 Size: 3713 AlwNI CAG_nnn'CTG Cuts at: 363 420 592 664 1018 2585 363 Size: 57 172 72 354 1567 1491 Fragments arranged by size: 1567 1491 354 172 72 57 ApaLI G'TGCA_C Cuts at: 2483 2483 Size: 3713 ApoI r'AATT_y Cuts at: 717 3066 3322 717 Size: 2349 256 1108 Fragments arranged by size: 2349 1108 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3713 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3713 AvaI C'yCGr_G Cuts at: 733 733 Size: 3713 BamHI G'GATC_C Cuts at: 738 738 Size: 3713 BanI G'GyrC_C Cuts at: 729 1143 1391 1426 729 Size: 414 248 35 3016 Fragments arranged by size: 3016 414 248 35 BanII G_rGCy'C Cuts at: 126 635 727 1757 126 Size: 509 92 1030 2082 Fragments arranged by size: 2082 1030 509 92 BbeI G_GCGC'C Cuts at: 1147 1395 1147 Size: 248 3465 BciVI GTATCCnnnnn_n' Cuts at: 1611 2372 3713 1611 Size: 761 1341 1611 Fragments arranged by size: 1611 1341 761 BclI T'GATC_A Cuts at: 1233 1233 Size: 3713 BfrBI ATG'CAT Cuts at: 291 291 Size: 3713 BglII A'GATC_T Cuts at: 473 1228 3265 473 Size: 755 2037 921 Fragments arranged by size: 2037 921 755 BlpI GC'TnA_GC Cuts at: 825 825 Size: 3713 Bme1580I G_kGCm'C Cuts at: 1338 1431 2487 1338 Size: 93 1056 2564 Fragments arranged by size: 2564 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 629 925 1006 1341 3485 629 Size: 296 81 335 2144 857 Fragments arranged by size: 2144 857 335 296 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 528 2080 528 Size: 1552 2161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 517 517 Size: 3713 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 337 507 889 1768 2260 2558 337 Size: 170 382 879 492 298 1492 Fragments arranged by size: 1492 879 492 382 298 170 BsaI GGTCTCn'nnnn_ Cuts at: 3417 3417 Size: 3713 BsaAI yAC'GTr Cuts at: 1066 1066 Size: 3713 BsaBI GATnn'nnATC Cuts at: 6 1227 3264 6 Size: 1221 2037 455 Fragments arranged by size: 2037 1221 455 BsaHI Gr'CG_yC Cuts at: 622 1144 1392 2094 3590 622 Size: 522 248 702 1496 745 Fragments arranged by size: 1496 745 702 522 248 BsaWI w'CCGG_w Cuts at: 446 902 1423 2101 2375 2522 2933 446 Size: 456 521 678 274 147 411 1226 Fragments arranged by size: 1226 678 521 456 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 367 397 460 490 520 367 Size: 30 63 30 30 3560 Fragments arranged by size: 3560 63 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 451 514 517 451 Size: 63 3 3647 Fragments arranged by size: 3647 63 3 BseYI C'CCAG_C Cuts at: 113 1138 1896 2473 2969 113 Size: 1025 758 577 496 857 Fragments arranged by size: 1025 857 758 577 496 BsiEI CG_ry'CG Cuts at: 104 447 1255 1301 2509 104 Size: 343 808 46 1208 1308 Fragments arranged by size: 1308 1208 808 343 46 BsiHKAI G_wGCw'C Cuts at: 704 727 1505 1695 2487 704 Size: 23 778 190 792 1930 Fragments arranged by size: 1930 792 778 190 23 BsmBI CGTCTCn'nnnn_ Cuts at: 136 235 461 136 Size: 99 226 3388 Fragments arranged by size: 3388 226 99 Bsp1286I G_dGCh'C Cuts at: 126 635 704 727 1338 1431 1505 1695 1757 Size: 509 69 23 611 93 74 190 62 Cuts at: 1757 2487 126 Size: 730 1352 Fragments arranged by size: 1352 730 611 509 190 93 74 69 62 23 BspEI T'CCGG_A Cuts at: 2933 2933 Size: 3713 BspHI T'CATG_A Cuts at: 2889 2889 Size: 3713 BspMI ACCTGCnnnn'nnnn_ Cuts at: 237 554 763 977 1279 1660 237 Size: 317 209 214 302 381 2290 Fragments arranged by size: 2290 381 317 302 214 209 BsrBI CCG'CTC Cuts at: 50 2005 2059 3543 3708 50 Size: 1955 54 1484 165 55 Fragments arranged by size: 1955 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1625 1625 Size: 3713 BsrFI r'CCGG_y Cuts at: 319 1711 1892 2101 319 Size: 1392 181 209 1931 Fragments arranged by size: 1931 1392 209 181 BssSI C'ACGA_G Cuts at: 1984 2342 1984 Size: 358 3355 BstAPI GCAn_nnn'nTGC Cuts at: 664 1090 664 Size: 426 3287 BstBI TT'CG_AA Cuts at: 2074 2074 Size: 3713 BstXI CCAn_nnnn'nTGG Cuts at: 682 682 Size: 3713 BstYI r'GATC_y Cuts at: 1 473 738 1205 1228 1563 1809 2821 2907 Size: 472 265 467 23 335 246 1012 86 Cuts at: 2907 2919 3265 1 Size: 12 346 449 Fragments arranged by size: 1012 472 467 449 346 335 265 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3713 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1663 1844 2007 1663 Size: 181 163 3369 Fragments arranged by size: 3369 181 163 BtsI GCAGTG_nn' Cuts at: 427 956 427 Size: 529 3184 DraI TTT'AAA Cuts at: 712 712 Size: 3713 DrdI GACnn_nn'nnGTC Cuts at: 249 1419 2277 249 Size: 1170 858 1685 Fragments arranged by size: 1685 1170 858 EaeI y'GGCC_r Cuts at: 1298 1472 1863 1890 3235 1298 Size: 174 391 27 1345 1776 Fragments arranged by size: 1776 1345 391 174 27 EagI C'GGCC_G Cuts at: 1298 1298 Size: 3713 EarI CTCTTCn'nnn_ Cuts at: 1736 1946 1736 Size: 210 3503 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1921 2231 2377 3523 1921 Size: 310 146 1146 2111 Fragments arranged by size: 2111 1146 310 146 EcoICRI GAG'CTC Cuts at: 725 725 Size: 3713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 193 517 1538 1970 2717 2985 193 Size: 324 1021 432 747 268 921 Fragments arranged by size: 1021 921 747 432 324 268 EcoO109I rG'GnC_Cy Cuts at: 852 852 Size: 3713 EcoRI G'AATT_C Cuts at: 717 717 Size: 3713 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1732 1732 Size: 3713 FspI TGC'GCA Cuts at: 424 1494 424 Size: 1070 2643 HaeII r_GCGC'y Cuts at: 1147 1395 2417 3396 1147 Size: 248 1022 979 1464 Fragments arranged by size: 1464 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 172 204 367 399 458 460 490 492 574 Size: 32 163 32 59 2 30 2 82 Cuts at: 574 589 606 621 1555 1585 1587 1617 172 Size: 15 17 15 934 30 2 30 2268 Fragments arranged by size: 2268 934 163 82 59 32 32 30 30 30 17 15 15 2 2 2 HincII GTy'rAC Cuts at: 752 752 Size: 3713 HindIII A'AGCT_T Cuts at: 560 560 Size: 3713 KasI G'GCGC_C Cuts at: 1143 1391 1143 Size: 248 3465 KpnI G_GTAC'C Cuts at: 733 733 Size: 3713 MluI A'CGCG_T Cuts at: 3146 3146 Size: 3713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 371 2384 2568 371 Size: 2013 184 1516 Fragments arranged by size: 2013 1516 184 MscI TGG'CCA Cuts at: 1474 1474 Size: 3713 MslI CAynn'nnrTG Cuts at: 674 680 1702 1829 674 Size: 6 1022 127 2558 Fragments arranged by size: 2558 1022 127 6 MspA1I CmG'CkG Cuts at: 420 824 1138 1498 2511 2756 420 Size: 404 314 360 1013 245 1377 Fragments arranged by size: 1377 1013 404 360 314 245 NaeI GCC'GGC Cuts at: 321 1894 321 Size: 1573 2140 NarI GG'CG_CC Cuts at: 1144 1392 1144 Size: 248 3465 NcoI C'CATG_G Cuts at: 107 107 Size: 3713 NgoMIV G'CCGG_C Cuts at: 319 1892 319 Size: 1573 2140 NsiI A_TGCA'T Cuts at: 293 293 Size: 3713 NspI r_CATG'y Cuts at: 295 766 1266 2173 295 Size: 471 500 907 1835 Fragments arranged by size: 1835 907 500 471 PciI A'CATG_T Cuts at: 2169 2169 Size: 3713 PmeI GTTT'AAAC Cuts at: 712 712 Size: 3713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2852 2884 2852 Size: 32 3681 PstI C_TGCA'G Cuts at: 155 434 760 1445 155 Size: 279 326 685 2423 Fragments arranged by size: 2423 685 326 279 PvuI CG_AT'CG Cuts at: 104 104 Size: 3713 PvuII CAG'CTG Cuts at: 420 1138 1498 420 Size: 718 360 2635 Fragments arranged by size: 2635 718 360 RsrII CG'GwC_CG Cuts at: 1908 1908 Size: 3713 SacI G_AGCT'C Cuts at: 727 727 Size: 3713 SalI G'TCGA_C Cuts at: 750 750 Size: 3713 SapI GCTCTTCn'nnn_ Cuts at: 1736 1946 1736 Size: 210 3503 SbfI CC_TGCA'GG Cuts at: 760 760 Size: 3713 ScaI AGT'ACT Cuts at: 3230 3230 Size: 3713 SfcI C'TryA_G Cuts at: 33 151 430 649 756 1441 2434 2625 33 Size: 118 279 219 107 685 993 191 1121 Fragments arranged by size: 1121 993 685 279 219 191 118 107 SfoI GGC'GCC Cuts at: 1145 1393 1145 Size: 248 3465 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3713 SmaI CCC'GGG Cuts at: 735 735 Size: 3713 SmlI C'TyrA_G Cuts at: 352 522 868 1783 2275 2537 352 Size: 170 346 915 492 262 1528 Fragments arranged by size: 1528 915 492 346 262 170 SpeI A'CTAG_T Cuts at: 67 67 Size: 3713 SphI G_CATG'C Cuts at: 766 1266 766 Size: 500 3213 StyI C'CwwG_G Cuts at: 107 847 1201 107 Size: 740 354 2619 Fragments arranged by size: 2619 740 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1296 1429 1888 2097 1296 Size: 133 459 209 2912 Fragments arranged by size: 2912 459 209 133 TatI w'GTAC_w Cuts at: 3228 3228 Size: 3713 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3230 3262 3230 Size: 32 3681 Tth111I GACn'n_nGTC Cuts at: 1510 1510 Size: 3713 XbaI T'CTAG_A Cuts at: 744 744 Size: 3713 XcmI CCAnnnn_n'nnnnTGG Cuts at: 676 1208 676 Size: 532 3181 XmaI C'CCGG_G Cuts at: 733 733 Size: 3713 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI