Restriction map for
pF1KB6878
(Linear) (Six-base) MAPSORT of: KIBB6878.seq Check: 9623 from: 1 to: 600
KIBB6878.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 129 600
Size: 129 471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 600
Size: 85 515
AhdI GACnn_n'nnGTC
Cuts at: 0 78 600
Size: 78 522
AlwNI CAG_nnn'CTG
Cuts at: 0 255 312 484 556 600
Size: 255 57 172 72 44
Fragments arranged by size:
255 172 72 57 44
BanII G_rGCy'C
Cuts at: 0 18 527 600
Size: 18 509 73
Fragments arranged by size:
509 73 18
BfrBI ATG'CAT
Cuts at: 0 183 600
Size: 183 417
BglII A'GATC_T
Cuts at: 0 365 600
Size: 365 235
BmrI ACTGGGnnnn_n'
Cuts at: 0 521 600
Size: 521 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 600
Size: 420 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 600
Size: 409 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 399 600
Size: 229 170 201
Fragments arranged by size:
229 201 170
BsaHI Gr'CG_yC
Cuts at: 0 514 600
Size: 514 86
BsaWI w'CCGG_w
Cuts at: 0 338 600
Size: 338 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 352 382 412 600
Size: 259 30 63 30 30 188
Fragments arranged by size:
259 188 63 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 406 409 600
Size: 343 63 3 191
Fragments arranged by size:
343 191 63 3
BseYI C'CCAG_C
Cuts at: 0 5 600
Size: 5 595
BsiEI CG_ry'CG
Cuts at: 0 339 600
Size: 339 261
BsiHKAI G_wGCw'C
Cuts at: 0 596 600
Size: 596 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 28 127 353 600
Size: 28 99 226 247
Fragments arranged by size:
247 226 99 28
Bsp1286I G_dGCh'C
Cuts at: 0 18 527 596 600
Size: 18 509 69 4
Fragments arranged by size:
509 69 18 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 446 600
Size: 129 317 154
Fragments arranged by size:
317 154 129
BsrFI r'CCGG_y
Cuts at: 0 211 600
Size: 211 389
BstAPI GCAn_nnn'nTGC
Cuts at: 0 556 600
Size: 556 44
BstXI CCAn_nnnn'nTGG
Cuts at: 0 574 600
Size: 574 26
BstYI r'GATC_y
Cuts at: 0 365 600
Size: 365 235
BtsI GCAGTG_nn'
Cuts at: 0 319 600
Size: 319 281
DrdI GACnn_nn'nnGTC
Cuts at: 0 141 600
Size: 141 459
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 409 600
Size: 85 324 191
Fragments arranged by size:
324 191 85
FspI TGC'GCA
Cuts at: 0 316 600
Size: 316 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 259 291 350 352 382 384
Size: 64 32 163 32 59 2 30 2
Cuts at: 384 466 481 498 513 600
Size: 82 15 17 15 87
Fragments arranged by size:
163 87 82 64 59 32 32 30
17 15 15 2 2
HindIII A'AGCT_T
Cuts at: 0 452 600
Size: 452 148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 600
Size: 263 337
MslI CAynn'nnrTG
Cuts at: 0 566 572 600
Size: 566 6 28
Fragments arranged by size:
566 28 6
MspA1I CmG'CkG
Cuts at: 0 312 600
Size: 312 288
NaeI GCC'GGC
Cuts at: 0 213 600
Size: 213 387
NgoMIV G'CCGG_C
Cuts at: 0 211 600
Size: 211 389
NsiI A_TGCA'T
Cuts at: 0 185 600
Size: 185 415
NspI r_CATG'y
Cuts at: 0 187 600
Size: 187 413
PstI C_TGCA'G
Cuts at: 0 47 326 600
Size: 47 279 274
Fragments arranged by size:
279 274 47
PvuII CAG'CTG
Cuts at: 0 312 600
Size: 312 288
SfcI C'TryA_G
Cuts at: 0 43 322 541 600
Size: 43 279 219 59
Fragments arranged by size:
279 219 59 43
SmlI C'TyrA_G
Cuts at: 0 244 414 600
Size: 244 170 186
Fragments arranged by size:
244 186 170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 568 600
Size: 568 32
Enzymes that do cut:
AarI AcuI AhdI AlwNI BanII BfrBI BglII BmrI
BplI BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstAPI BstXI
BstYI BtsI DrdI Eco57MI FspI Hin4I HindIII MmeI
MslI MspA1I NaeI NgoMIV NsiI NspI PstI PvuII
SfcI SmlI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI
BmtI Bpu10I BsaI BsaAI BsaBI BsgI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NdeI
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI