(Circular) (Six-base) MAPSORT of: pF1KE0011.seq Check: 1893 from: 1 to: 5552 pF1KE0011 5552 bp With 182 enzymes: SgfI * April 24, 2009 13:09 .. AatII G_ACGT'C Cuts at: 1865 1865 Size: 5552 AccI GT'mk_AC Cuts at: 493 2590 493 Size: 2097 3455 Acc65I G'GTAC_C Cuts at: 2568 2568 Size: 5552 AclI AA'CG_TT Cuts at: 5392 5392 Size: 5552 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 755 1147 1786 2023 2080 2440 3377 3809 4556 Size: 392 639 237 57 360 937 432 747 Cuts at: 4556 4824 755 Size: 268 1483 Fragments arranged by size: 1483 937 747 639 432 392 360 268 237 57 AfeI AGC'GCT Cuts at: 1391 1391 Size: 5552 AflIII A'CryG_T Cuts at: 2904 4008 4985 2904 Size: 1104 977 3471 Fragments arranged by size: 3471 1104 977 AgeI A'CCGG_T Cuts at: 3940 3940 Size: 5552 AlwNI CAG_nnn'CTG Cuts at: 1764 1890 2857 4424 1764 Size: 126 967 1567 2892 Fragments arranged by size: 2892 1567 967 126 ApaLI G'TGCA_C Cuts at: 2044 4322 2044 Size: 2278 3274 ApoI r'AATT_y Cuts at: 2556 4905 5161 2556 Size: 2349 256 2947 Fragments arranged by size: 2947 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5552 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5552 AvaI C'yCGr_G Cuts at: 1871 2572 1871 Size: 701 4851 BamHI G'GATC_C Cuts at: 2577 2577 Size: 5552 BanI G'GyrC_C Cuts at: 361 2568 2982 3230 3265 361 Size: 2207 414 248 35 2648 Fragments arranged by size: 2648 2207 414 248 35 BanII G_rGCy'C Cuts at: 314 335 1105 1566 1925 2566 3596 314 Size: 21 770 461 359 641 1030 2270 Fragments arranged by size: 2270 1030 770 641 461 359 21 BbeI G_GCGC'C Cuts at: 2986 3234 2986 Size: 248 5304 BbsI GAAGACnn'nnnn_ Cuts at: 1585 1757 1585 Size: 172 5380 BbvCI CC'TCA_GC Cuts at: 772 1393 2104 772 Size: 621 711 4220 Fragments arranged by size: 4220 711 621 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1363 1397 1824 1858 1363 Size: 34 427 34 5057 Fragments arranged by size: 5057 427 34 34 BciVI GTATCCnnnnn_n' Cuts at: 731 1137 3450 4211 5552 731 Size: 406 2313 761 1341 731 Fragments arranged by size: 2313 1341 761 731 406 BclI T'GATC_A Cuts at: 3072 3072 Size: 5552 BglII A'GATC_T Cuts at: 3067 5104 3067 Size: 2037 3515 BlpI GC'TnA_GC Cuts at: 994 2008 2664 994 Size: 1014 656 3882 Fragments arranged by size: 3882 1014 656 Bme1580I G_kGCm'C Cuts at: 2048 3177 3270 4326 2048 Size: 1129 93 1056 3274 Fragments arranged by size: 3274 1129 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 995 2764 2845 3180 5324 995 Size: 1769 81 335 2144 1223 Fragments arranged by size: 2144 1769 1223 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2532 3919 2532 Size: 1387 4165 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 160 586 921 1538 1843 1996 2017 2502 160 Size: 426 335 617 305 153 21 485 3210 Fragments arranged by size: 3210 617 485 426 335 305 153 21 Bpu10I CC'TnA_GC Cuts at: 772 1393 2104 772 Size: 621 711 4220 Fragments arranged by size: 4220 711 621 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1540 1771 2467 2728 3607 4099 4397 1540 Size: 231 696 261 879 492 298 2695 Fragments arranged by size: 2695 879 696 492 298 261 231 BsaI GGTCTCn'nnnn_ Cuts at: 749 1514 2458 5256 749 Size: 765 944 2798 1045 Fragments arranged by size: 2798 1045 944 765 BsaAI yAC'GTr Cuts at: 2905 2905 Size: 5552 BsaBI GATnn'nnATC Cuts at: 6 3066 5103 6 Size: 3060 2037 455 Fragments arranged by size: 3060 2037 455 BsaHI Gr'CG_yC Cuts at: 1862 2983 3231 3933 5429 1862 Size: 1121 248 702 1496 1985 Fragments arranged by size: 1985 1496 1121 702 248 BsaWI w'CCGG_w Cuts at: 830 878 2741 3262 3940 4214 4361 4772 830 Size: 48 1863 521 678 274 147 411 1610 Fragments arranged by size: 1863 1610 678 521 411 274 147 48 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 140 170 140 Size: 30 5522 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 703 897 1432 1954 2089 2521 2524 703 Size: 194 535 522 135 432 3 3731 Fragments arranged by size: 3731 535 522 432 194 135 3 BseYI C'CCAG_C Cuts at: 766 979 1961 2166 2977 3735 4312 4808 766 Size: 213 982 205 811 758 577 496 1510 Fragments arranged by size: 1510 982 811 758 577 496 213 205 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 828 940 1684 2220 828 Size: 112 744 536 4160 Fragments arranged by size: 4160 744 536 112 BsiEI CG_ry'CG Cuts at: 104 3094 3140 4348 104 Size: 2990 46 1208 1308 Fragments arranged by size: 2990 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 314 335 662 1000 1286 1566 1925 2014 2048 Size: 21 327 338 286 280 359 89 34 Cuts at: 2048 2057 2566 3344 3534 4326 314 Size: 9 509 778 190 792 1540 Fragments arranged by size: 1540 792 778 509 359 338 327 286 280 190 89 34 21 9 BsmI GAATG_Cn' Cuts at: 855 1596 855 Size: 741 4811 BsmBI CGTCTCn'nnnn_ Cuts at: 178 178 Size: 5552 Bsp1286I G_dGCh'C Cuts at: 314 335 662 1000 1105 1286 1566 1925 2014 Size: 21 327 338 105 181 280 359 89 Cuts at: 2014 2048 2057 2566 3177 3270 3344 3534 3596 Size: 34 9 509 611 93 74 190 62 Cuts at: 3596 4326 314 Size: 730 1540 Fragments arranged by size: 1540 730 611 509 359 338 327 280 190 181 105 93 89 74 62 34 21 9 BspEI T'CCGG_A Cuts at: 878 4772 878 Size: 3894 1658 BspHI T'CATG_A Cuts at: 248 1566 1610 4728 248 Size: 1318 44 3118 1072 Fragments arranged by size: 3118 1318 1072 44 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2602 2816 3118 3499 2602 Size: 214 302 381 4655 Fragments arranged by size: 4655 381 302 214 BsrBI CCG'CTC Cuts at: 50 867 1495 3844 3898 5382 5547 50 Size: 817 628 2349 54 1484 165 55 Fragments arranged by size: 2349 1484 817 628 165 55 54 BsrDI GCAATG_nn' Cuts at: 323 1020 1344 3464 323 Size: 697 324 2120 2411 Fragments arranged by size: 2411 2120 697 324 BsrFI r'CCGG_y Cuts at: 872 1651 3550 3731 3940 872 Size: 779 1899 181 209 2484 Fragments arranged by size: 2484 1899 779 209 181 BssSI C'ACGA_G Cuts at: 424 3823 4181 424 Size: 3399 358 1795 Fragments arranged by size: 3399 1795 358 BstAPI GCAn_nnn'nTGC Cuts at: 552 2094 2929 552 Size: 1542 835 3175 Fragments arranged by size: 3175 1542 835 BstBI TT'CG_AA Cuts at: 3913 3913 Size: 5552 BstXI CCAn_nnnn'nTGG Cuts at: 1137 1320 1137 Size: 183 5369 BstYI r'GATC_y Cuts at: 1 1213 1893 2577 3044 3067 3402 3648 4660 Size: 1212 680 684 467 23 335 246 1012 Cuts at: 4660 4746 4758 5104 1 Size: 86 12 346 449 Fragments arranged by size: 1212 1012 684 680 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 497 611 1646 2447 107 Size: 390 114 1035 801 3212 Fragments arranged by size: 3212 1035 801 390 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1011 3502 3683 3846 1011 Size: 2491 181 163 2717 Fragments arranged by size: 2717 2491 181 163 BtsI GCAGTG_nn' Cuts at: 531 1140 1426 1913 2316 2795 531 Size: 609 286 487 403 479 3288 Fragments arranged by size: 3288 609 487 479 403 286 ClaI AT'CG_AT Cuts at: 1844 1844 Size: 5552 DraI TTT'AAA Cuts at: 2551 2551 Size: 5552 DrdI GACnn_nn'nnGTC Cuts at: 3258 4116 3258 Size: 858 4694 EaeI y'GGCC_r Cuts at: 218 614 708 945 1090 1649 1928 1946 2444 Size: 396 94 237 145 559 279 18 498 Cuts at: 2444 3137 3311 3702 3729 5074 218 Size: 693 174 391 27 1345 696 Fragments arranged by size: 1345 696 693 559 498 396 391 279 237 174 145 94 27 18 EagI C'GGCC_G Cuts at: 3137 3137 Size: 5552 EarI CTCTTCn'nnn_ Cuts at: 586 731 773 857 3575 3785 586 Size: 145 42 84 2718 210 2353 Fragments arranged by size: 2718 2353 210 145 84 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3760 4070 4216 5362 3760 Size: 310 146 1146 3950 Fragments arranged by size: 3950 1146 310 146 EcoICRI GAG'CTC Cuts at: 312 333 1564 1923 2564 312 Size: 21 1231 359 641 3300 Fragments arranged by size: 3300 1231 641 359 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 160 586 755 921 1147 1538 1786 1843 1996 Size: 426 169 166 226 391 248 57 153 Cuts at: 1996 2017 2023 2080 2440 2502 3377 3809 4556 Size: 21 6 57 360 62 875 432 747 Cuts at: 4556 4824 160 Size: 268 888 Fragments arranged by size: 888 875 747 432 426 391 360 268 248 226 169 166 153 62 57 57 21 6 EcoO109I rG'GnC_Cy Cuts at: 198 714 931 1508 1992 2691 198 Size: 516 217 577 484 699 3059 Fragments arranged by size: 3059 699 577 516 484 217 EcoRI G'AATT_C Cuts at: 2556 2556 Size: 5552 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3571 3571 Size: 5552 FspI TGC'GCA Cuts at: 3333 3333 Size: 5552 HaeII r_GCGC'y Cuts at: 1393 1453 2986 3234 4256 5235 1393 Size: 60 1533 248 1022 979 1710 Fragments arranged by size: 1710 1533 1022 979 248 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 336 368 373 392 405 424 451 483 520 Size: 32 5 19 13 19 27 32 37 Cuts at: 520 552 1205 1237 1878 1910 2146 2178 3394 Size: 32 653 32 641 32 236 32 1216 Cuts at: 3394 3424 3426 3456 336 Size: 30 2 30 2432 Fragments arranged by size: 2432 1216 653 641 236 37 32 32 32 32 32 32 30 30 27 19 19 13 5 2 HincII GTy'rAC Cuts at: 432 2591 432 Size: 2159 3393 KasI G'GCGC_C Cuts at: 2982 3230 2982 Size: 248 5304 KpnI G_GTAC'C Cuts at: 2572 2572 Size: 5552 MluI A'CGCG_T Cuts at: 4985 4985 Size: 5552 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1712 2343 4223 4407 1712 Size: 631 1880 184 2857 Fragments arranged by size: 2857 1880 631 184 MscI TGG'CCA Cuts at: 220 1930 2446 3313 220 Size: 1710 516 867 2459 Fragments arranged by size: 2459 1710 867 516 MslI CAynn'nnrTG Cuts at: 263 1360 1549 1930 2531 3541 3668 263 Size: 1097 189 381 601 1010 127 2147 Fragments arranged by size: 2147 1097 1010 601 381 189 127 MspA1I CmG'CkG Cuts at: 599 1585 1628 2091 2120 2663 2977 3337 4350 Size: 986 43 463 29 543 314 360 1013 Cuts at: 4350 4595 599 Size: 245 1556 Fragments arranged by size: 1556 1013 986 543 463 360 314 245 43 29 NaeI GCC'GGC Cuts at: 1653 3733 1653 Size: 2080 3472 NarI GG'CG_CC Cuts at: 2983 3231 2983 Size: 248 5304 NcoI C'CATG_G Cuts at: 107 497 2447 107 Size: 390 1950 3212 Fragments arranged by size: 3212 1950 390 NgoMIV G'CCGG_C Cuts at: 1651 3731 1651 Size: 2080 3472 NspI r_CATG'y Cuts at: 1359 2605 3105 4012 1359 Size: 1246 500 907 2899 Fragments arranged by size: 2899 1246 907 500 PasI CC'CwG_GG Cuts at: 1670 1670 Size: 5552 PciI A'CATG_T Cuts at: 4008 4008 Size: 5552 PflMI CCAn_nnn'nTGG Cuts at: 1890 1890 Size: 5552 PfoI T'CCnGG_A Cuts at: 269 857 269 Size: 588 4964 PmeI GTTT'AAAC Cuts at: 2551 2551 Size: 5552 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 4691 Size: 32 5520 PpuMI rG'GwC_Cy Cuts at: 198 714 931 1508 198 Size: 516 217 577 4242 Fragments arranged by size: 4242 577 516 217 PshAI GACnn'nnGTC Cuts at: 469 469 Size: 5552 PstI C_TGCA'G Cuts at: 526 548 604 1660 2337 2599 3284 526 Size: 22 56 1056 677 262 685 2794 Fragments arranged by size: 2794 1056 685 677 262 56 22 PvuI CG_AT'CG Cuts at: 104 104 Size: 5552 PvuII CAG'CTG Cuts at: 599 1585 1628 2977 3337 599 Size: 986 43 1349 360 2814 Fragments arranged by size: 2814 1349 986 360 43 RsrII CG'GwC_CG Cuts at: 3747 3747 Size: 5552 SacI G_AGCT'C Cuts at: 314 335 1566 1925 2566 314 Size: 21 1231 359 641 3300 Fragments arranged by size: 3300 1231 641 359 21 SalI G'TCGA_C Cuts at: 2589 2589 Size: 5552 SapI GCTCTTCn'nnn_ Cuts at: 586 857 3575 3785 586 Size: 271 2718 210 2353 Fragments arranged by size: 2718 2353 271 210 SbfI CC_TGCA'GG Cuts at: 2599 2599 Size: 5552 ScaI AGT'ACT Cuts at: 388 5069 388 Size: 4681 871 SexAI A'CCwGG_T Cuts at: 2039 2372 2039 Size: 333 5219 SfcI C'TryA_G Cuts at: 33 522 544 600 910 1656 2333 2595 3280 Size: 489 22 56 310 746 677 262 685 Cuts at: 3280 4273 4464 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 746 685 677 489 310 262 191 56 22 SfoI GGC'GCC Cuts at: 2984 3232 2984 Size: 248 5304 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5552 SmaI CCC'GGG Cuts at: 2574 2574 Size: 5552 SmlI C'TyrA_G Cuts at: 1555 1786 2482 2707 3622 4114 4376 1555 Size: 231 696 225 915 492 262 2731 Fragments arranged by size: 2731 915 696 492 262 231 225 SpeI A'CTAG_T Cuts at: 67 67 Size: 5552 SphI G_CATG'C Cuts at: 1359 2605 3105 1359 Size: 1246 500 3806 Fragments arranged by size: 3806 1246 500 StuI AGG'CCT Cuts at: 1603 2035 1603 Size: 432 5120 StyI C'CwwG_G Cuts at: 107 221 497 1319 1598 2288 2447 2686 3040 Size: 114 276 822 279 690 159 239 354 Cuts at: 3040 107 Size: 2619 Fragments arranged by size: 2619 822 690 354 279 276 239 159 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3135 3268 3727 3936 3135 Size: 133 459 209 4751 Fragments arranged by size: 4751 459 209 133 TatI w'GTAC_w Cuts at: 386 1409 5067 386 Size: 1023 3658 871 Fragments arranged by size: 3658 1023 871 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 140 172 1276 1308 2047 2079 5069 5101 140 Size: 32 1104 32 739 32 2990 32 591 Fragments arranged by size: 2990 1104 739 591 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1065 3349 1065 Size: 2284 3268 XbaI T'CTAG_A Cuts at: 2583 2583 Size: 5552 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1824 2370 3047 1824 Size: 546 677 4329 Fragments arranged by size: 4329 677 546 XmaI C'CCGG_G Cuts at: 2572 2572 Size: 5552 ZraI GAC'GTC Cuts at: 1863 1863 Size: 5552 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI