(Linear) (Six-base) MAPSORT of: KIEE0011.seq Check: 4454 from: 1 to: 2439 KIEE0011.orf With 182 enzymes: SgfI * April 24, 2009 13:09 .. AatII G_ACGT'C Cuts at: 0 1757 2439 Size: 1757 682 AccI GT'mk_AC Cuts at: 0 385 2439 Size: 385 2054 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 647 1039 1678 1915 1972 2332 2439 Size: 647 392 639 237 57 360 107 Fragments arranged by size: 647 639 392 360 237 107 57 AfeI AGC'GCT Cuts at: 0 1283 2439 Size: 1283 1156 AlwNI CAG_nnn'CTG Cuts at: 0 1656 1782 2439 Size: 1656 126 657 Fragments arranged by size: 1656 657 126 ApaLI G'TGCA_C Cuts at: 0 1936 2439 Size: 1936 503 AvaI C'yCGr_G Cuts at: 0 1763 2439 Size: 1763 676 BanI G'GyrC_C Cuts at: 0 253 2439 Size: 253 2186 BanII G_rGCy'C Cuts at: 0 206 227 997 1458 1817 2439 Size: 206 21 770 461 359 622 Fragments arranged by size: 770 622 461 359 206 21 BbsI GAAGACnn'nnnn_ Cuts at: 0 1477 1649 2439 Size: 1477 172 790 Fragments arranged by size: 1477 790 172 BbvCI CC'TCA_GC Cuts at: 0 664 1285 1996 2439 Size: 664 621 711 443 Fragments arranged by size: 711 664 621 443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1255 1289 1716 1750 2439 Size: 1255 34 427 34 689 Fragments arranged by size: 1255 689 427 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 623 1029 2439 Size: 623 406 1410 Fragments arranged by size: 1410 623 406 BlpI GC'TnA_GC Cuts at: 0 886 1900 2439 Size: 886 1014 539 Fragments arranged by size: 1014 886 539 Bme1580I G_kGCm'C Cuts at: 0 1940 2439 Size: 1940 499 BmrI ACTGGGnnnn_n' Cuts at: 0 887 2439 Size: 887 1552 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2424 2439 Size: 2424 15 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 478 813 1430 1735 1888 1909 2394 Size: 52 426 335 617 305 153 21 485 Cuts at: 2394 2439 Size: 45 Fragments arranged by size: 617 485 426 335 305 153 52 45 21 Bpu10I CC'TnA_GC Cuts at: 0 664 1285 1996 2439 Size: 664 621 711 443 Fragments arranged by size: 711 664 621 443 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1432 1663 2359 2439 Size: 1432 231 696 80 Fragments arranged by size: 1432 696 231 80 BsaI GGTCTCn'nnnn_ Cuts at: 0 641 1406 2350 2439 Size: 641 765 944 89 Fragments arranged by size: 944 765 641 89 BsaHI Gr'CG_yC Cuts at: 0 1754 2439 Size: 1754 685 BsaWI w'CCGG_w Cuts at: 0 722 770 2439 Size: 722 48 1669 Fragments arranged by size: 1669 722 48 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 32 62 2439 Size: 32 30 2377 Fragments arranged by size: 2377 32 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 595 789 1324 1846 1981 2413 2416 2439 Size: 595 194 535 522 135 432 3 23 Fragments arranged by size: 595 535 522 432 194 135 23 3 BseYI C'CCAG_C Cuts at: 0 658 871 1853 2058 2439 Size: 658 213 982 205 381 Fragments arranged by size: 982 658 381 213 205 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 720 832 1576 2112 2439 Size: 720 112 744 536 327 Fragments arranged by size: 744 720 536 327 112 BsiHKAI G_wGCw'C Cuts at: 0 206 227 554 892 1178 1458 1817 1906 Size: 206 21 327 338 286 280 359 89 Cuts at: 1906 1940 1949 2439 Size: 34 9 490 Fragments arranged by size: 490 359 338 327 286 280 206 89 34 21 9 BsmI GAATG_Cn' Cuts at: 0 747 1488 2439 Size: 747 741 951 Fragments arranged by size: 951 747 741 BsmBI CGTCTCn'nnnn_ Cuts at: 0 70 2439 Size: 70 2369 Bsp1286I G_dGCh'C Cuts at: 0 206 227 554 892 997 1178 1458 1817 Size: 206 21 327 338 105 181 280 359 Cuts at: 1817 1906 1940 1949 2439 Size: 89 34 9 490 Fragments arranged by size: 490 359 338 327 280 206 181 105 89 34 21 9 BspEI T'CCGG_A Cuts at: 0 770 2439 Size: 770 1669 BspHI T'CATG_A Cuts at: 0 140 1458 1502 2439 Size: 140 1318 44 937 Fragments arranged by size: 1318 937 140 44 BsrBI CCG'CTC Cuts at: 0 759 1387 2439 Size: 759 628 1052 Fragments arranged by size: 1052 759 628 BsrDI GCAATG_nn' Cuts at: 0 215 912 1236 2439 Size: 215 697 324 1203 Fragments arranged by size: 1203 697 324 215 BsrFI r'CCGG_y Cuts at: 0 764 1543 2439 Size: 764 779 896 Fragments arranged by size: 896 779 764 BssSI C'ACGA_G Cuts at: 0 316 2439 Size: 316 2123 BstAPI GCAn_nnn'nTGC Cuts at: 0 444 1986 2439 Size: 444 1542 453 Fragments arranged by size: 1542 453 444 BstXI CCAn_nnnn'nTGG Cuts at: 0 1029 1212 2439 Size: 1029 183 1227 Fragments arranged by size: 1227 1029 183 BstYI r'GATC_y Cuts at: 0 1105 1785 2439 Size: 1105 680 654 Fragments arranged by size: 1105 680 654 BtgI C'CryG_G Cuts at: 0 389 503 1538 2339 2439 Size: 389 114 1035 801 100 Fragments arranged by size: 1035 801 389 114 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 903 2439 Size: 903 1536 BtsI GCAGTG_nn' Cuts at: 0 423 1032 1318 1805 2208 2439 Size: 423 609 286 487 403 231 Fragments arranged by size: 609 487 423 403 286 231 ClaI AT'CG_AT Cuts at: 0 1736 2439 Size: 1736 703 EaeI y'GGCC_r Cuts at: 0 110 506 600 837 982 1541 1820 1838 Size: 110 396 94 237 145 559 279 18 Cuts at: 1838 2336 2439 Size: 498 103 Fragments arranged by size: 559 498 396 279 237 145 110 103 94 18 EarI CTCTTCn'nnn_ Cuts at: 0 478 623 665 749 2439 Size: 478 145 42 84 1690 Fragments arranged by size: 1690 478 145 84 42 EcoICRI GAG'CTC Cuts at: 0 204 225 1456 1815 2439 Size: 204 21 1231 359 624 Fragments arranged by size: 1231 624 359 204 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 52 478 647 813 1039 1430 1678 1735 Size: 52 426 169 166 226 391 248 57 Cuts at: 1735 1888 1909 1915 1972 2332 2394 2439 Size: 153 21 6 57 360 62 45 Fragments arranged by size: 426 391 360 248 226 169 166 153 62 57 57 52 45 21 6 EcoO109I rG'GnC_Cy Cuts at: 0 90 606 823 1400 1884 2439 Size: 90 516 217 577 484 555 Fragments arranged by size: 577 555 516 484 217 90 HaeII r_GCGC'y Cuts at: 0 1285 1345 2439 Size: 1285 60 1094 Fragments arranged by size: 1285 1094 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 228 260 265 284 297 316 343 375 Size: 228 32 5 19 13 19 27 32 Cuts at: 375 412 444 1097 1129 1770 1802 2038 2070 Size: 37 32 653 32 641 32 236 32 Cuts at: 2070 2439 Size: 369 Fragments arranged by size: 653 641 369 236 228 37 32 32 32 32 32 32 27 19 19 13 5 HincII GTy'rAC Cuts at: 0 324 2439 Size: 324 2115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1604 2235 2439 Size: 1604 631 204 Fragments arranged by size: 1604 631 204 MscI TGG'CCA Cuts at: 0 112 1822 2338 2439 Size: 112 1710 516 101 Fragments arranged by size: 1710 516 112 101 MslI CAynn'nnrTG Cuts at: 0 155 1252 1441 1822 2423 2439 Size: 155 1097 189 381 601 16 Fragments arranged by size: 1097 601 381 189 155 16 MspA1I CmG'CkG Cuts at: 0 491 1477 1520 1983 2012 2439 Size: 491 986 43 463 29 427 Fragments arranged by size: 986 491 463 427 43 29 NaeI GCC'GGC Cuts at: 0 1545 2439 Size: 1545 894 NcoI C'CATG_G Cuts at: 0 389 2339 2439 Size: 389 1950 100 Fragments arranged by size: 1950 389 100 NgoMIV G'CCGG_C Cuts at: 0 1543 2439 Size: 1543 896 NspI r_CATG'y Cuts at: 0 1251 2439 Size: 1251 1188 PasI CC'CwG_GG Cuts at: 0 1562 2439 Size: 1562 877 PflMI CCAn_nnn'nTGG Cuts at: 0 1782 2439 Size: 1782 657 PfoI T'CCnGG_A Cuts at: 0 161 749 2439 Size: 161 588 1690 Fragments arranged by size: 1690 588 161 PpuMI rG'GwC_Cy Cuts at: 0 90 606 823 1400 2439 Size: 90 516 217 577 1039 Fragments arranged by size: 1039 577 516 217 90 PshAI GACnn'nnGTC Cuts at: 0 361 2439 Size: 361 2078 PstI C_TGCA'G Cuts at: 0 418 440 496 1552 2229 2439 Size: 418 22 56 1056 677 210 Fragments arranged by size: 1056 677 418 210 56 22 PvuII CAG'CTG Cuts at: 0 491 1477 1520 2439 Size: 491 986 43 919 Fragments arranged by size: 986 919 491 43 SacI G_AGCT'C Cuts at: 0 206 227 1458 1817 2439 Size: 206 21 1231 359 622 Fragments arranged by size: 1231 622 359 206 21 SapI GCTCTTCn'nnn_ Cuts at: 0 478 749 2439 Size: 478 271 1690 Fragments arranged by size: 1690 478 271 ScaI AGT'ACT Cuts at: 0 280 2439 Size: 280 2159 SexAI A'CCwGG_T Cuts at: 0 1931 2264 2439 Size: 1931 333 175 Fragments arranged by size: 1931 333 175 SfcI C'TryA_G Cuts at: 0 414 436 492 802 1548 2225 2439 Size: 414 22 56 310 746 677 214 Fragments arranged by size: 746 677 414 310 214 56 22 SmlI C'TyrA_G Cuts at: 0 1447 1678 2374 2439 Size: 1447 231 696 65 Fragments arranged by size: 1447 696 231 65 SphI G_CATG'C Cuts at: 0 1251 2439 Size: 1251 1188 StuI AGG'CCT Cuts at: 0 1495 1927 2439 Size: 1495 432 512 Fragments arranged by size: 1495 512 432 StyI C'CwwG_G Cuts at: 0 113 389 1211 1490 2180 2339 2439 Size: 113 276 822 279 690 159 100 Fragments arranged by size: 822 690 279 276 159 113 100 TatI w'GTAC_w Cuts at: 0 278 1301 2439 Size: 278 1023 1138 Fragments arranged by size: 1138 1023 278 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 32 64 1168 1200 1939 1971 2439 Size: 32 32 1104 32 739 32 468 Fragments arranged by size: 1104 739 468 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 957 2439 Size: 957 1482 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1716 2262 2439 Size: 1716 546 177 Fragments arranged by size: 1716 546 177 ZraI GAC'GTC Cuts at: 0 1755 2439 Size: 1755 684 Enzymes that do cut: AatII AccI AcuI AfeI AlwNI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BciVI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PshAI PstI PvuII SacI SapI ScaI SexAI SfcI SmlI SphI StuI StyI TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BclI BfrBI BglI BglII BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BspMI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII XbaI XhoI XmaI XmnI