Restriction map for pF1KE0413
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvaII       
AviII       BbeI        BbrPI       BbvCI       BciVI       
BclI        BglI        BglII       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmFI       BspDI       BspEI       BspMI       
BsrBI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CfoI        Cfr13I      ClaI        CpoI        
DraI        DraII       DsaI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HphI        KasI        KpnI        
KspI        MaeII       MamI        MluI        MroI        
MslI        MvnI        NarI        NcoI        NdeI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        Sau96I      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AciI            9  GCGG                 CCGC(-3/-1)
AflII         825  CTTAAG               C^TTAAG
AflIII        666  ACATGT               A^C[GA][CT]GT
AhdI          986  GACAAACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         986  GACAAACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI           40  CCCGGG               C^[CT]CG[GA]G
AvrII         852  CCTAGG               C^CTAGG
BamHI         720  GGATCC               G^GATCC
BanI          103  GGTGCC               G^G[CT][GA]CC
BanII          19  GAGCTC               G[GA]GC[CT]^C
BcgI          576  CGAAGAAAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          825  CTTAAG               C^TTAAG
BlnI          852  CCTAGG               C^CTAGG
BlpI          877  GCTCAGC              GC^T[ACGT]AGC
BpmI          511  CTGGAG               CTGGAG(16/14)
Bpu1102I      877  GCTCAGC              GC^T[ACGT]AGC
BsaI          901  GAGACC               GGTCTC(1/5)
BseRI          61  GAGGAG               GAGGAG(10/8)
BsmI          713  GAATGC               GAATGC(1/-1)
BsoBI          40  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      129  TGTACA               T^GTACA
BspHI         246  TCATGA               T^CATGA
BspLU11I      666  ACATGT               A^CATGT
BsrDI         755  GCAATG               GCAATG(2/0)
BsrFI         692  GCCGGC               [GA]^CCGG[CT]
BsrGI         129  TGTACA               T^GTACA
BstBI         932  TTCGAA               TT^CGAA
BstNI         510  CCTGG                CC^[AT]GG
BstYI         720  GGATCC               [GA]^GATC[CT]
CelII         877  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        692  GCCGGC               [GA]^CCGG[CT]
DraIII        684  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          841  GACAGCCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      986  GACAAACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         847  CCTGTCCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        510  CCTGG                ^CC[AT]GG
FauI            9  GCGGG                CCCGC(4/6)
HincII         70  GTTAAC               GT[CT]^[GA]AC
HindII         70  GTTAAC               GT[CT]^[GA]AC
HpaI           70  GTTAAC               GTT^AAC
MfeI          791  CAATTG               C^AATTG
MflI          720  GGATCC               [GA]^GATC[CT]
MspA1I        371  CAGCTG               C[AC]G^C[GT]G
MunI          791  CAATTG               C^AATTG
MvaI          510  CCTGG                CC^[AT]GG
NaeI          692  GCCGGC               GCC^GGC
NgoMIV        692  GCCGGC               G^CCGGC
NheI          699  GCTAGC               G^CTAGC
NspV          932  TTCGAA               TT^CGAA
PvuII         371  CAGCTG               CAG^CTG
RcaI          246  TCATGA               T^CATGA
SacI           19  GAGCTC               GAGCT^C
SapI          182  GAAGAGC              GCTCTTC(1/4)
SfuI          932  TTCGAA               TT^CGAA
SmaI           40  CCCGGG               CCC^GGG
SmlI          825  CTTAAG               C^T[CT][GA]AG
SphI          159  GCATGC               GCATG^C
SspBI         129  TGTACA               T^GTACA
XhoII         720  GGATCC               [GA]^GATC[CT]
XmaCI          40  CCCGGG               C^CCGGG
XmaI           40  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI          130  GTAC                 GT^AC
              271  GTAC                
AspHI          19  GAGCTC               G[AT]GC[AT]^C
              857  GAGCAC              
BalI          680  TGGCCA               TGG^CCA
              915  TGGCCA              
BbsI          290  GAAGAC               GAAGAC(2/6)
              448  GAAGAC              
BcnI           40  CCCGG                CC^[GC]GG
               41  CCGGG               
BmyI           19  GAGCTC               G[AGT]GC[ACT]^C
              857  GAGCAC              
BpuAI         290  GAAGAC               GAAGAC(2/6)
              448  GAAGAC              
BsiHKAI        19  GAGCTC               G[AT]GC[AT]^C
              857  GAGCAC              
BsiYI         740  CCTTTCACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              847  CCTGTCCTAGG         
BslI          740  CCTTTCACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              847  CCTGTCCTAGG         
BsmAI         901  GAGAC                GTCTC(1/5)
              984  GAGAC               
Bsp1286I       19  GAGCTC               G[AGT]GC[ACT]^C
              857  GAGCAC              
BstF5I         75  CATCC                GGATG(2/0)
              342  GGATG               
DpnII         640  GATC                 ^GATC
              721  GATC                
EaeI          680  TGGCCA               [CT]^GGCC[GA]
              915  TGGCCA              
EarI          182  GAAGAG               CTCTTC(1/4)
              708  GAAGAG              
EcoT14I       852  CCTAGG               C^C[AT][AT]GG
              905  CCTTGG              
FokI           75  CATCC                GGATG(9/13)
              342  GGATG               
HapII          41  CCGG                 C^CGG
              693  CCGG                
HpaII          41  CCGG                 C^CGG
              693  CCGG                
Ksp632I       182  GAAGAG               CTCTTC(1/4)
              708  GAAGAG              
MboI          640  GATC                 ^GATC
              721  GATC                
MluNI         680  TGGCCA               TGG^CCA
              915  TGGCCA              
MscI          680  TGGCCA               TGG^CCA
              915  TGGCCA              
MspI           41  CCGG                 C^CGG
              693  CCGG                
MwoI          361  GCAAAGGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              376  GCTGTTGGAGC         
NciI           40  CCCGG                CC^[GC]GG
               41  CCGGG               
NdeII         640  GATC                 ^GATC
              721  GATC                
RsaI          130  GTAC                 GT^AC
              271  GTAC                
Sau3AI        640  GATC                 ^GATC
              721  GATC                
StyI          852  CCTAGG               C^C[AT][AT]GG
              905  CCTTGG              
TaqI          575  TCGA                 T^CGA
              933  TCGA                
Tsp45I        589  GTGAC                ^GT[GC]AC
              821  GTCAC               
TthHB8I       575  TCGA                 T^CGA
              933  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AcsI        AluI        AlwI        AlwNI       ApoI        
BbvI        BfaI        BsaJI       BsgI        BsrI        
BssKI       Cac8I       DdeI        Fnu4HI      HaeIII      
HinfI       ItaI        MaeI        MaeIII      MboII       
MnlI        MseI        NlaIII      NlaIV       NspI        
ScrFI       SfaNI       TfiI        Tru9I       TseI        
Tsp509I     TspRI       
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