Restriction map for pF1KE0413
 (Linear) (Six-base) MAPSORT of: KIEE0413.seq  Check: 1154  from: 1  to: 1002



KIEE0413.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AflII C'TTAA_G

Cuts at:      0     825    1002 
   Size:        825     177
 
AflIII A'CryG_T

Cuts at:      0     666    1002 
   Size:        666     336
 
AhdI GACnn_n'nnGTC

Cuts at:      0     991    1002 
   Size:        991      11
 
AlwNI CAG_nnn'CTG

Cuts at:      0     322     376     592    1002 
   Size:        322      54     216     410

  Fragments arranged by size: 

                 410     322     216      54
 
ApoI r'AATT_y

Cuts at:      0     139     196     354     468    1002 
   Size:        139      57     158     114     534

  Fragments arranged by size: 

                 534     158     139     114      57
 
AvaI C'yCGr_G

Cuts at:      0      40    1002 
   Size:         40     962
 
AvrII C'CTAG_G

Cuts at:      0     852    1002 
   Size:        852     150
 
BamHI G'GATC_C

Cuts at:      0     720    1002 
   Size:        720     282
 
BanI G'GyrC_C

Cuts at:      0     103    1002 
   Size:        103     899
 
BanII G_rGCy'C

Cuts at:      0      23    1002 
   Size:         23     979
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     297     455    1002 
   Size:        297     158     547

  Fragments arranged by size: 

                 547     297     158
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     565     599    1002 
   Size:        565      34     403

  Fragments arranged by size: 

                 565     403      34
 
BlpI GC'TnA_GC

Cuts at:      0     878    1002 
   Size:        878     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     119    1002 
   Size:        119     883
 
BmtI G_CTAG'C

Cuts at:      0     703    1002 
   Size:        703     299
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     406     893    1002 
   Size:        406     487     109

  Fragments arranged by size: 

                 487     406     109
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532    1002 
   Size:        532     470
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     895    1002 
   Size:        895     107
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     518     548    1002 
   Size:        518      30     454

  Fragments arranged by size: 

                 518     454      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76    1002 
   Size:         76     926
 
BseYI C'CCAG_C

Cuts at:      0     814    1002 
   Size:        814     188
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     605     631    1002 
   Size:        299     306      26     371

  Fragments arranged by size: 

                 371     306     299      26
 
BsiHKAI G_wGCw'C

Cuts at:      0      23     861    1002 
   Size:         23     838     141

  Fragments arranged by size: 

                 838     141      23
 
BsmI GAATG_Cn'

Cuts at:      0     719    1002 
   Size:        719     283
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     861    1002 
   Size:         23     838     141

  Fragments arranged by size: 

                 838     141      23
 
BspHI T'CATG_A

Cuts at:      0     246    1002 
   Size:        246     756
 
BsrDI GCAATG_nn'

Cuts at:      0     762    1002 
   Size:        762     240
 
BsrFI r'CCGG_y

Cuts at:      0     692    1002 
   Size:        692     310
 
BsrGI T'GTAC_A

Cuts at:      0     129    1002 
   Size:        129     873
 
BstBI TT'CG_AA

Cuts at:      0     933    1002 
   Size:        933      69
 
BstYI r'GATC_y

Cuts at:      0     720    1002 
   Size:        720     282
 
BtsI GCAGTG_nn'

Cuts at:      0     593    1002 
   Size:        593     409
 
DraIII CAC_nnn'GTG

Cuts at:      0     689    1002 
   Size:        689     313
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     847    1002 
   Size:        847     155
 
EaeI y'GGCC_r

Cuts at:      0     680     915    1002 
   Size:        680     235      87

  Fragments arranged by size: 

                 680     235      87
 
EarI CTCTTCn'nnn_

Cuts at:      0     177     703    1002 
   Size:        177     526     299

  Fragments arranged by size: 

                 526     299     177
 
EcoICRI GAG'CTC

Cuts at:      0      21    1002 
   Size:         21     981
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532    1002 
   Size:        532     470
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     851    1002 
   Size:        851     151
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     756     970    1002 
   Size:        756     214      32

  Fragments arranged by size: 

                 756     214      32
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342     977    1002 
   Size:        310      32     635      25

  Fragments arranged by size: 

                 635     310      32      25
 
HincII GTy'rAC

Cuts at:      0      72    1002 
   Size:         72     930
 
HpaI GTT'AAC

Cuts at:      0      72    1002 
   Size:         72     930
 
MfeI C'AATT_G

Cuts at:      0     791    1002 
   Size:        791     211
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     197     360    1002 
   Size:        197     163     642

  Fragments arranged by size: 

                 642     197     163
 
MscI TGG'CCA

Cuts at:      0     682     917    1002 
   Size:        682     235      85

  Fragments arranged by size: 

                 682     235      85
 
MspA1I CmG'CkG

Cuts at:      0     373    1002 
   Size:        373     629
 
NaeI GCC'GGC

Cuts at:      0     694    1002 
   Size:        694     308
 
NgoMIV G'CCGG_C

Cuts at:      0     692    1002 
   Size:        692     310
 
NheI G'CTAG_C

Cuts at:      0     699    1002 
   Size:        699     303
 
NspI r_CATG'y

Cuts at:      0     163     670     788    1002 
   Size:        163     507     118     214

  Fragments arranged by size: 

                 507     214     163     118
 
PciI A'CATG_T

Cuts at:      0     666    1002 
   Size:        666     336
 
PfoI T'CCnGG_A

Cuts at:      0     509    1002 
   Size:        509     493
 
PvuII CAG'CTG

Cuts at:      0     373    1002 
   Size:        373     629
 
SacI G_AGCT'C

Cuts at:      0      23    1002 
   Size:         23     979
 
SapI GCTCTTCn'nnn_

Cuts at:      0     177    1002 
   Size:        177     825
 
SmaI CCC'GGG

Cuts at:      0      42    1002 
   Size:         42     960
 
SmlI C'TyrA_G

Cuts at:      0     825    1002 
   Size:        825     177
 
SphI G_CATG'C

Cuts at:      0     163    1002 
   Size:        163     839
 
StyI C'CwwG_G

Cuts at:      0     852     905    1002 
   Size:        852      53      97

  Fragments arranged by size: 

                 852      97      53
 
TatI w'GTAC_w

Cuts at:      0     129     270    1002 
   Size:        129     141     732

  Fragments arranged by size: 

                 732     141     129
 
XmaI C'CCGG_G

Cuts at:      0      40    1002 
   Size:         40     962
 

 Enzymes that do cut:

    AflII   AflIII     AhdI    AlwNI     ApoI     AvaI    AvrII    BamHI
     BanI    BanII     BbsI     BcgI     BlpI     BmrI     BmtI     BplI
     BpmI     BsaI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrDI    BsrFI    BsrGI    BstBI    BstYI     BtsI
   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI     FalI
    Hin4I   HincII     HpaI     MfeI     MmeI     MscI   MspA1I     NaeI
   NgoMIV     NheI     NspI     PciI     PfoI    PvuII     SacI     SapI
     SmaI     SmlI     SphI     StyI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     BaeI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI   BssHII    BssSI
   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI     EagI     EciI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MluI     MslI
     NarI     NcoI     NdeI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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