Restriction map for pF1KE0112
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbsI        BciVI       BclI        BfrI        
BglII       BlnI        BpmI        BpuAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MroI        MseI        
MslI        MspA1I      MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII          69  CGCG                 CG^CG
AcsI          271  AAATTC               [GA]^AATT[CT]
AluI           13  AGCT                 AG^CT
Aor51HI       568  AGCGCT               AGC^GCT
ApaI          508  GGGCCC               GGGCC^C
ApoI          271  AAATTC               [GA]^AATT[CT]
AspHI          12  GAGCTC               G[AT]GC[AT]^C
AvaII         237  GGTCC                G^G[AT]CC
BalI           93  TGGCCA               TGG^CCA
BanI          394  GGTGCC               G^G[CT][GA]CC
BbrPI          97  CACGTG               CAC^GTG
BbvCI         220  GCTGAGG              CCTCAGC(-5/-2)
BcgI          408  CGAGGGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          571  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      571  GCTGAGC              GC^T[ACGT]AGC
BsaAI          97  CACGTG               [CT]AC^GT[GA]
BsaI          250  GAGACC               GGTCTC(1/5)
BsgI          485  GTGCAG               GTGCAG(16/14)
BsiHKAI        12  GAGCTC               G[AT]GC[AT]^C
BsmFI         238  GTCCC                GGGAC(10/14)
BsrBI         300  GAGCGG               CCGCTC(-3/-3)
BsrDI         107  GCAATG               GCAATG(2/0)
BsrI          181  ACTGG                ACTGG(1/-1)
BstF5I        527  CATCC                GGATG(2/0)
BstUI          69  CGCG                 CG^CG
BstXI          84  CCAGGAGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         571  GCTGAGC              GC^T[ACGT]AGC
DpnII         390  GATC                 ^GATC
Eco47III      568  AGCGCT               AGC^GCT
FauI           67  CCCGC                CCCGC(4/6)
FokI          527  CATCC                GGATG(9/13)
MaeIII         37  GTGAC                ^GT[ACGT]AC
MboI          390  GATC                 ^GATC
MluNI          93  TGGCCA               TGG^CCA
MscI           93  TGGCCA               TGG^CCA
MvnI           69  CGCG                 CG^CG
NdeII         390  GATC                 ^GATC
PleI          139  GAGTC                GAGTC(4/5)
PmaCI          97  CACGTG               CAC^GTG
PmlI           97  CACGTG               CAC^GTG
PpuMI         236  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          399  CTGCAG               CTGCA^G
SacI           12  GAGCTC               GAGCT^C
SapI          370  GAAGAGC              GCTCTTC(1/4)
Sau3AI        390  GATC                 ^GATC
SfaNI         187  GATGC                GCATC(5/9)
SmaI           26  CCCGGG               CCC^GGG
TaqI          194  TCGA                 T^CGA
TfiI          343  GATTC                G^A[AT]TC
Tsp45I         37  GTGAC                ^GT[GC]AC
TthHB8I       194  TCGA                 T^CGA
XmaCI          26  CCCGGG               C^CCGGG
XmaI           26  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Asp700I       267  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              285  GAAGCCTTTC          
AvaI           26  CCCGGG               C^[CT]CG[GA]G
              537  CTCGGG              
BanII          12  GAGCTC               G[GA]GC[CT]^C
              508  GGGCCC              
BfaI          209  CTAG                 C^TAG
              482  CTAG                
BglI           43  GCCGAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              466  GCCAAGGGGGC         
BseRI         354  CTCCTC               GAGGAG(10/8)
               46  GAGGAG              
BsmAI         113  GAGAC                GTCTC(1/5)
              250  GAGAC               
BsoBI          26  CCCGGG               C^[CT]CG[GA]G
              537  CTCGGG              
CfoI           59  GCGC                 GCG^C
              569  GCGC                
EaeI           81  CGGCCA               [CT]^GGCC[GA]
               93  TGGCCA              
EarI          370  GAAGAG               CTCTTC(1/4)
              499  GAAGAG              
HaeII          58  GGCGCT               [GA]GCGC^[CT]
              568  AGCGCT              
HhaI           59  GCGC                 GCG^C
              569  GCGC                
HinP1I         59  GCGC                 G^CGC
              569  GCGC                
HinfI         139  GAGTC                G^A[ACGT]TC
              343  GATTC               
Ksp632I       370  GAAGAG               CTCTTC(1/4)
              499  GAAGAG              
MaeI          209  CTAG                 C^TAG
              482  CTAG                
MaeII          35  ACGT                 A^CGT
               98  ACGT                
SfcI          399  CTGCAG               C^T[GA][CT]AG
              518  CTACAG              
Tsp509I       272  AATT                 ^AATT
              296  AATT                
TspRI         180  CACTG                CA[GC]TG[ACGT][ACGT]^
              414  CACTG               
XmnI          267  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              285  GAAGCCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        BbvI        BcnI        BmyI        BsaJI       
BsiYI       BslI        Bsp1286I    BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DraII       EcoO109I    
EcoRII      EcoT14I     Fnu4HI      HaeIII      HapII       
HpaII       HphI        ItaI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NlaIV       
Sau96I      ScrFI       StyI        TseI        
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